COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae)

Authors

  • Renato Ribeiro Nogueira Ferraz Programa de Mestrado Profissional em Administração - Gestão em Sistemas de Saúde (PMPA-GSS) - Universidade Nove de Julho (UNINOVE)
  • Thales Kodi Namba
  • Carolina Alencar Nigro
  • Francisco Sandro Menezes Rodrigues
  • Anderson Sena Barnabé
  • João Victor Fornari

DOI:

https://doi.org/10.5216/cab.v15i3.28163

Keywords:

Aquaculture

Abstract

The demand and consumption of fish and their derivatives has increased considerably in recent years. However, fish are ideal hosts of numerous parasites, highlighting the need to develop new research methodologies for its detection. The aim of present study was to compare the efficacy of Ascocotyle metacercariae (Trematoda: Digenea) extraction from visceral tissues of Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae) by two methods: homogenization by blender or mixer. Twenty-six samples of M. liza were collected, being 16 liver samples and 10 samples of muscle tissue. Approximately 5g of each sample were processed by blender and mixer techniques homogenization for metacercariae extraction. In liver samples, up to 46 metacercariae were found in samples homogenized in blender. The lowest amount found was 2 metacercariae for blender and mixer techniques. In samples of muscle tissue, 4 metacercariae were observed in the mixer extraction. The lowest amount was found to be 2 parasites to blender and mixer. The mean metacercariae found and extracted from muscle tissue were 0.2 (+0.357) and 1.2 (+0.963) for blender and mixer, respectively. The averages of metacercariae found and extracted from fish liver, in blender and mixer, were 24 (+15.145) and 18 (+8.246), respectively. The homogenization techniques for blender and mixer were effective for the extraction of metacercariae of mullet fish tissues, suggesting that they may be directly applicable in the field of study, especially due to the ease of testing.

KEYWORDS: Epidemiology, health management, homogenization, Metacercariae, Mugil liza.

 

 

Downloads

Download data is not yet available.

Author Biography

Renato Ribeiro Nogueira Ferraz, Programa de Mestrado Profissional em Administração - Gestão em Sistemas de Saúde (PMPA-GSS) - Universidade Nove de Julho (UNINOVE)

Biólogo. Doutor em Ciências pela Universidade Federal de São Paulo (UNIFESP)

Downloads

Published

2014-09-27

How to Cite

FERRAZ, R. R. N.; NAMBA, T. K.; NIGRO, C. A.; RODRIGUES, F. S. M.; BARNABÉ, A. S.; FORNARI, J. V. COMPARISON BETWEEN TWO METHODS OF METACERCARIAE OF Ascocotyle sp (Trematoda: Digenea) EXTRACTION FROM TISSUES OF Mugil liza Valenciennes, 1836 (Teleostei: Mugilidae). Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 15, n. 3, p. 354–361, 2014. DOI: 10.5216/cab.v15i3.28163. Disponível em: https://revistas.ufg.br/vet/article/view/28163. Acesso em: 17 jul. 2024.

Issue

Vol. 15 No. 3 (2014)

Section

Veterinary Medicine

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).