PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC
DOI:
https://doi.org/10.5216/cab.v15i2.23017Keywords:
Animal sanityAbstract
The Neospora caninum is a protozoan Apicomplexa with greater involvement in abortions worldwide. The economic losses determined by neosporosis also include abortions besides the early disposal of cows, costs for replacing animals in the herd, drop in milk production as well as milk in fat production. The immunological diagnosis involves purchasing costly diagnostic kits on the market. Therefore, the aim of this study was the production of hybridomas secreting polyclonal antibodies with affinity to Neospora caninum (Nc-1 strain) for immunodiagnostic use. For antibodies production, we used sonicated protozoa from Vero cells in culture, purified by filtration. These tachyzoites were employed for immunization of BALB / c mice using saponin as adjuvant, which allowed obtaining polyclonal antibodies capable of revealing fluorescein reaction in indirect immunofluorescence. The fusion of splenic cells, from the immunized mice with myeloma cells SP2 / 0 resulted in 72.4% hybridomas secreting anti-Nc-1antibodies. These hybridomas secreted antibodies positive to N. caninum and negative to Toxoplasma gondii.
KEYWORDS: hybridomas, immunodiagnosis, monoclonal antibodies, neosporosis.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
