ENVIRONMENTAL CONTAMINATION OF VETERINARY HOSPITAL AND PROFILE OF ANTIMICROBIAL SUSCEPTIBILITY OF ISOLATED BACTERIA

Authors

  • Luciana Ruschel dos Santos UPF
  • João Francisco Scalco Neto UPF
  • Natalie Nadin Rizzo UPF
  • Paulo Vinícius Bastiani UPF
  • Laura Beatriz Rodrigues UPF
  • Heloisa Helena de Alcântara Barcellos UPF
  • Maurício Veloso Brun UPF

DOI:

https://doi.org/10.5216/cab.v11i2.2988

Keywords:

Animal Sanity

Abstract

This study assessed the contamination at the Veterinary Hospital of Passo Fundo University (HV-UPF), and the isolated bacteria were submitted to disinfectants efficacy tests and to antibiograms. Contamination was lower than 30 cfu/cm2 (air) and 2 cfu/cm2 (surfaces), within the limits established by APHA, but Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa, Escherichia coli, Enterobacter agglomerans and Arcanobacterium pyogenes were isolated. Alcohol 70%, quaternary ammonium 3.5% and sodium hypochlorite where tested. Sodium was ineffective against all bacteria, alcohol 70 % was ineffective against E. aglomerans and S. epidermidis after 30 s and quaternary ammonium 3.5% was ineffective against E.coli after 30 s, one and five minutes and P. aeruginosa after 30 s. The results of the antimicrobial susceptibility test performed on isolated strains of S. pidermidis revealed that 100% of the strains were resistant to penicillin G, oxacillin and chloramphenicol; 66.6% to ciprofloxacin, cefepime and rifamycin B, and 33.3% to erythromycin, sulfa+trimethoprim and gentamicin. The antimicrobial susceptibility test performed on other isolates showed that most of them were multiresistant, with resistance to three or more antimicrobials. The environmental contamination at HV-UPF can be reduced to acceptable levels through the cleaning procedures but bacteria with pathogenic potential and antimicrobial resistance are still isolated.

Keywords: Antimicrobial susceptibility, disinfectants, veterinary.

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Author Biography

Luciana Ruschel dos Santos, UPF

Possui graduação em Medicina Veterinária pela Pontifícia Universidade Católica do Rio Grande do Sul (1992), aperfeiçoamento em Patologia Aviária (1995) e Doutorado em Ciências Veterinárias pela Universidade Federal do Rio Grande do Sul (2001). Atualmente é professor da Universidade de Passo Fundo, das disciplinas de Microbiologia Geral, Tecnologia de Produtos de Origem Animal e Tecnologia de Carnes. Tem experiência na área de Medicina Veterinária, Agronomia e Engenharia de Alimentos, com ênfase em Microbiologia Geral, atuando principalmente nos seguintes temas: bacteriologia, diagnóstico microbiológico (PCR, RAPD/PCR, fagotipagem), métodos de desinfecção, esterilização e anti-sepsia, teste de eficiência de desinfetantes, resistência antimicrobiana, biofilmes, contaminação ambiental.

Published

2010-06-23

How to Cite

SANTOS, L. R. dos; SCALCO NETO, J. F.; RIZZO, N. N.; BASTIANI, P. V.; RODRIGUES, L. B.; BARCELLOS, H. H. de A.; BRUN, M. V. ENVIRONMENTAL CONTAMINATION OF VETERINARY HOSPITAL AND PROFILE OF ANTIMICROBIAL SUSCEPTIBILITY OF ISOLATED BACTERIA. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 2, p. 384–389, 2010. DOI: 10.5216/cab.v11i2.2988. Disponível em: https://revistas.ufg.br/vet/article/view/2988. Acesso em: 22 nov. 2024.

Issue

Section

Veterinary Medicine

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