EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS
DOI:
https://doi.org/10.5216/cab.v10i1.1932Keywords:
Reprodução AnimalAbstract
The purpose of this study was to evaluate the motility and viability of stallion semen cryopreserved with 5% dimethyl-formamyde as cryoprotectant and posterior dilution to 2.5 and 1.25% as final concentration of cryoprotectant. After thawing, samples were diluted with two commercial extenders (FR4® and Botucrio® ) in two moments: initial (Ti) and final (Tf). A total of 15 distinct ejaculates from five stallions of national breeds were analysed. Motility was observed using a computer assistant system analysis and viability was analyzed using fluorescent probes. A significant (P<0.05) increase on total and progressive motility parameters was observed at the final time using Botu-Crio® compared with FR4®. Statistical difference (P>0.05) on membrane integrity between treatments was not observed.KEY WORDS: Cryoprotectant, diluent, equine, freezing, thawing.
Downloads
Download data is not yet available.
Downloads
Published
2009-04-03
How to Cite
ZIMMERMANN, M. F.; FARINASSO, A.; MELO, C. M.; PAPA, F. O.; NEVES, J. P. EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 1, p. 238–245, 2009. DOI: 10.5216/cab.v10i1.1932. Disponível em: https://revistas.ufg.br/vet/article/view/1932. Acesso em: 5 nov. 2024.
Issue
Section
Veterinary Medicine
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).