METHYL-FORMAMIDE IN RAM SEMEN CRYOPRESERVATION

Authors

  • Carlos Pereira das Graças Autônomo
  • Alexandre In Piao G. Lim Médico veterinario autônomo
  • Andrei Antonioni G. Fidelis Pesquisador I da GENEAL, Genética Animal, Análise, Pesquisa e Laboratório S/A - Uberaba
  • Júlio Roquete Cardoso UFG Professor
  • Hélio Blume UPIS - União Pioneira de Integração social
  • Rafael Gianella Mondadori Universidade Federal de Pelotas - UFPel Professor

DOI:

https://doi.org/10.5216/cab.v14i4.17835

Keywords:

Animal reproduction

Abstract

The aim of this study was to evaluate the efficacy of methyl-formamide in ram semen cryopreservation. The semen pool used in this experiment was obtained by artificial vagina collection from four mixed breed Santa Inês rams, around four years of age. Semen collection was performed once a week, during six weeks. Each week corresponded to one experiment replication. The semen pool was divided in five fractions in order to be diluted in one of the following freezing media: (1) tris-egg yolk with 5.3% of glycerol (TG5.3G); (2) tris-egg yolk with 3% of methyl-formamide (TG3MF); tris-egg yolk with 5% of methyl-formamide (TG5MF); tris-egg yolk with 7% of methyl-formamide (TG7MF); tris-egg yolk with 9% of methyl-formamide (TG9MF). Semen progressive motility, vigor and thermoresistance were evaluated. The treatments TG5.3G (50%) and TG3MF (38%) showed higher progressive motility after thawing, while TG5MF (29%), TG7MF (1%) and TG9MF (6%) showerd lower motility. Freezing media containing methyl-formamide were less effective in preserving spermatozoa membrane integrity and morphology than control media. In TG7MF and TG9MF extenders, less than 60% spermatozoa showed normal morphology. After thermoresistance test, semen cryopreserved in TG3MF showed vigor (2.6) and motility (15%) statistically similar to TG5.3G media (15% and 2.8, respectively); however, the extender TG5.3G was more effective in preserving plasma membrane functional integrity. In conclusion, in the experimental conditions used, glycerol showed more cryoprotectant potential than methyl-formamide.

KEYWORDS: Amide; glycerol; ram; tris-egg yolk.

 

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Author Biographies

Carlos Pereira das Graças, Autônomo

Médico Veterinário. Atua como autônomo em assistencia técnica e extensão rural

Alexandre In Piao G. Lim, Médico veterinario autônomo

Graduado em Medicina Veterinária pela UPIS

Andrei Antonioni G. Fidelis, Pesquisador I da GENEAL, Genética Animal, Análise, Pesquisa e Laboratório S/A - Uberaba

Médico Veterinário, Mestrando em Ciências Veterinárias - UFU.Pesquisador I da GENEAL, Genética Animal, Análise, Pesquisa e Laboratório S/A - Uberaba

Júlio Roquete Cardoso, UFG Professor

Médico veterinário, Doutor em Biologia Celular.

Lotado no Instituto de Ciências Biológicas da UFG

Departamento de Morfologia

Área: anatomia animal

Hélio Blume, UPIS - União Pioneira de Integração social

Médico Veterinário, professor de Reprodução animal, Depto de Medicina Veterinária

Rafael Gianella Mondadori, Universidade Federal de Pelotas - UFPel Professor

Médico Veterinário, professor na área de Histologia Veterinária

Published

2013-12-17

How to Cite

DAS GRAÇAS, C. P.; LIM, A. I. P. G.; FIDELIS, A. A. G.; CARDOSO, J. R.; BLUME, H.; MONDADORI, R. G. METHYL-FORMAMIDE IN RAM SEMEN CRYOPRESERVATION. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 14, n. 4, p. 481–487, 2013. DOI: 10.5216/cab.v14i4.17835. Disponível em: https://revistas.ufg.br/vet/article/view/17835. Acesso em: 13 nov. 2024.

Issue

Section

Veterinary Medicine

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