Lack of mutagenicity of Solanum paniculatum L. in somatic cells of Drosophila melanogaster: SMART/wing

Authors

  • Vivian Ribeiro
  • Iara Lúcia Barbosa Fernandes Vieira
  • Débora Cristina Silva dos Passos
  • Elayne Miguel da Silva
  • Camila Regina do Vale
  • Leandro Prado Felício
  • Heleno Dias Ferreira
  • Pabline Marinho Vieira
  • Salvador de Carvalho

DOI:

https://doi.org/10.5216/rbn.v6i2.12851

Keywords:

Drosophila melanogaster, genotoxicity, jurubeba, SMART/wing, Solanum paniculatum L

Abstract

The lyophilized aqueous extract of Solanum paniculatum L. (jurubeba) leaves, a medicinal plant widely used by people as diuretic, in liver and spleen treatments, against fever and inflammation, underwent the SMART/wing test in somatic cells of Drosophila melanogaster for the evaluation of its mutagenic potential. We used three different concentrations of the leaf extract (2.5 mg.mL-1, 6.25 mg.mL-1, and 10 mg.mL-1), doxorubicin (DXR) as positive control, and distilled water with  5% sucrose as negative control. We employed three lineages of Drosophila in this experiment, flr³, ORR, and mwh. The  larvae derived from the standard cross (ST: flr³ females x mwh males) and the high bioactivation cross (HB: ORR females x mwh males) were chronically treated and the wings of the  emerging adults were analyzed. Our results showed no  mutagenic activity of S. paniculatum L. ST or HB crosses.

Downloads

Download data is not yet available.

References

Balunas, M. J. & A. D. Kinghorn. 2005. Drug discovery from medicinal plants. Life Sci. 78: 431-441.

Bernardes, A. 1984. A pocketbook of Brazilian herbs. 2a ed., Editora das Artes Ltda., Rio de janeiro.

Blankemeyer, J. T., M. L. McWilliams, J. R. Rayburn, M. Weissenberg & M. Friedman. 1998. Developmental toxicology of solamargine and solasonine glycoalkaloids in frog embryos. Food Chem. Toxicol. 36: 383-389.

Caldeira, K. S. 2008. Avaliação citotóxica, genotóxica e moduladora do anorexígeno dietilpropiona em células somáticas de Drosophila melanogaster: SMART/asa. Dissertação de Mestrado. Universidade Federal de Goiás, Goiânia.

Coimbra, R. 1994. Manual de fitoterapia. 2a ed., CEJUP, Belém.

Costa, W. F. & J. C. Nepomuceno. 2003. Efeito protetor do chá de cogumelo do sol (Agaricus blazei Murill) contra a ação genotóxica do uretano em células somáticas de Drosophila melanogaster. Rev. Ciênc. Farmac. 24: 153-158.

Cruz, G. L. 1995. Dicionário das plantas úteis no Brasil. 5a ed., Editora Bertrand, Rio de Janeiro.

Forni-Martins, E. R., M. C. M. Marques & M. R. Lemes. 1998. Biologia floral e reprodução de Solanum paniculatum L. (Solanaceae) no estado de São Paulo, Brasil. Rev. Bras. Bot. 21: 117-124.

Frei H. & F. E. Würgler 1988. Statistical methods to decide whether mutagenicity test data from Drosophila assays indicate a positive, negative, or inconclusive result. Mutat. Res. 203: 297-308.

Frei, H. & F. E. Würgler. 1992. Optimal experimental design and simple size for the statistical evaluation of data from somatic mutation and recombination test (SMART) in Drosophila. Mutat. Res. 334: 247-258.

Garcia, J., T. K. B. Jacobson, J. G. Farias & R. F. Boaventura. 2008. Effectiveness of methods to increase the germination rate of jurubeba (Solanum paniculatum L.) seeds. Pesq. Agropec. Trop. 38: 223-226.

Graf, U., F. E. Würgler, A. J. Katz, H. Frei, H. Juon, C. B. Hall & P. G. Kale. 1984. Somatic mutation and recombination test in Drosophila melanogaster. Environ. Mutagen. 6: 153-188.

Graf, U., H. Frei, A. Kagi, A.J. Katz & F. E. Würgler. 1989. Thirty compounds tested in the Drosophila wing spot test. Mutat. Res. 222: 359-373.

Graf, U. & N. van Schaik. 1992. Improved high bioactivation cross for the wing mutation and recombination test in Drosophila melanogaster. Mutat. Res. 271: 59-67.

Guzmán-Rincon, J. & U. Graf. 1995. Drosophila melanogaster somatic mutation and recombination test as a biomonitor, p. 169-181. In: F. M. Butterworth, L. A. Corkum & J. Guzmán-Rincon (Eds.), Biomonitors and biomarkers as indicators of environmental change. Plenum, New York.

Ito, H., K. Shimura, H. Itoh & M. Kawade. 1997. Antitumor effects of a new polysaccharide protein complex (ATOM) prepared from Agaricus blazei (Iwade Strain 101) “Himematsutake” and its mechanisms in tumor-bearing mice. Anticanc. Res. 17: 277-284.

Lorenzi, H. 2000. Plantas daninhas do Brasil – terrestres, aquáticas, parasitas e tóxicas, 3a ed., Nova Odessa, Instituto Plantarum.

Lorenzi, H. & F. J. Matos. 2002. Plantas medicinais no Brasil: nativas e exóticas cultivadas. Instituto Plantarum, Nova Odessa, 2002.

Marques, E. K., M. Napp, H. Wingue & A. R. Cordeiro. 1966. A corn meal, soybean flour, wheat germ medium for Drosophila. Dros. Inf. Serv. 41: 187.

Newman, D. J., G. M. Cragg & K. M. Snader. 2003. Natural products as sources of new drugs over the period 1981-2002. J. Nat. Prod. 66: 1022-1037.

Nunes, A. P. M. & A. C. Araujo. 2003. Ausência de genotoxicidade do esteviosídeo em E. coli. In: X Semana de Iniciação Cientifica da UERJ, Rio de Janeiro, Anais, p. 15.

Ripperger, H. 1967a. Isolation of neochlorogenin and painculogenin form Solanum paniculatum L. Chem. Ber. 100: 1741-1752.

Ripperger, H. 1967b. Jurubin, a nitrogen containing steroidsaponin of a new structural type from Solanum paniculatum L. concerning the structure of paniculidin. Chem. Ber. 100: 1725-1740.

Ripperger, H. 1968. Structure of paniculonin A and B, two new spirostane glycosides from Solanum paniculatum L. Chem Ber. 101: 2450-2458.

Ripperger, H. & K. Screiber. 1981. Solanum steroidal alkaloids, p. 81–192. In: R. H. F. Manske (Ed.), The alkaloids, v. XIX. Academic Press, New York.

Scarpato, R., L. Pistelli, A. Bertoli, E. Nieiri & L. Migliore. 1998. In vitro genotoxicity and cytotoxicity of five new chemical compounds of plant origin by means of the human lymphocyte micronucleus assay. Toxicol. in Vitro 12: 153-161.

Schwontkowski, D. 1993. Herbs of the Amazon: traditional and common uses. Science Student Brain Trust Publishing, Utah.

Simões, C. M. O., E. P. Schenkel, G. Gosmann, J. C. P. Mello, L. A. Mentz & P. R. Petrovick. 2000. Farmacognosia: da planta ao medicamento. 2a ed., Ed. UFRGS/ Ed. UFSC, Porto Alegre/Florianopólis.

Vieira, P. M. 2008. Estudo do potencial genotóxico/mutagênico e antigenotóxico/antimutagênico de Solanum paniculatum L. Dissertação de Mestrado, Universidade Federal de Goiás, Goiânia.

Würgler, F. E., F. H. Sobels & E. Vogel. 1984. Drosophila as an assay system for detecting genetic changes, p. 555-601. In: B. J. Kilbey, M. Legator, & W. Nichols (Eds.), Handbook of mutagenicity test procedure. 2nd ed., Elsevier, Amsterdam.

Downloads

Published

2010-12-29

How to Cite

RIBEIRO, V.; VIEIRA, I. L. B. F.; PASSOS, D. C. S. dos; SILVA, E. M. da; VALE, C. R. do; FELÍCIO, L. P.; FERREIRA, H. D.; VIEIRA, P. M.; CARVALHO, S. de. Lack of mutagenicity of Solanum paniculatum L. in somatic cells of Drosophila melanogaster: SMART/wing. Revista de Biologia Neotropical / Journal of Neotropical Biology, Goiânia, v. 6, n. 2, p. 27–33, 2010. DOI: 10.5216/rbn.v6i2.12851. Disponível em: https://revistas.ufg.br/RBN/article/view/12851. Acesso em: 30 jun. 2024.

Issue

Vol. 6 No. 2 (2009)

Section

Articles

License

The expontaneos submmition of the manuscript automaticaly implies in the cession of all patrimonial rights for the Journal of Neotropical Bilogy (RBN) after publication. The autor allow the right of first publication of the article to the RBN, under Creative Commons Attribution 4.0 (CC BY-NC 4.0) Licence.

There are garanties for the authors to the authorial and moral rights, for each one of the articles published by RBN, with permissions:

1. The use of article and contents for the education and researches.

2. The use of the article and their contents, linking to the Article on the web site of the RBN, allowing the divulgation on:

  • institutional closed web (intranet).
  • open access repositories.

3. Preparation and divulgation of the other publication derived from the article and its content, if there is citation of the original publication by RBN.

4. Make printed copies in small quatinties for personal use.

Most read articles by the same author(s)