Probiotic and dietary fermented milk drink made from goat's milk with added seriguela pulp: processing and analysis

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Abstract

Abstract: The search for a better quality of life has driven the food market to develop new functional products whose composition reduces or eliminates nutrients that can cause problems to consumer health, such as sugar, as well as the use of less allergenic matrices. Therefore, the objective of the present work was to develop a probiotic and dietary fermented dairy drink made from goat's milk with the addition of seriguela pulp, as well as analyzing its physicochemical, microbiological and sensory characteristics. The formulations were processed using the sweeteners thaumatin and sucralose. The probiotic used was Lactobacillus acidophilus and its viability was evaluated for a period of 28 days. Regarding the physical and chemical characteristics, there was a reduction in pH and an increase in acidity during storage of the dairy drink. The results of the analyses for molds, yeasts, and coagulase-positive Staphylococcus were within the limits recommended by law. The probiotic strain used remained active during the 28 days of storage (9 log CFU/mL). The formulations prepared with sweeteners presented a higher sensory acceptance index than the control formulation with sugar, indicating that the replacement of sucrose with high and low intensity sweeteners did not negatively interfere in the sensory perception of the tasters. The probiotic and dietary fermented milk drink developed proved to be an excellent innovative option for the functional foods market, for those intolerant/allergic to cow's milk and for consumers with sugar metabolism problems.

Keywords: Functional food; dairy matrix; no added sugar; Lactobacillus acidophilus; Streptococcus thermophilus e Lactobacillus bulgaricus.

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References

1. Kashyap PC, Chia N, Nelson H, Segal E, Elinav E. Microbiome at the frontier of personalized Medicine. Mayo Clinic Proceedings. 2017; 92 (12), 1855–1864. https://doi.org/10.1016/j.mayocp.2017.10.004

2. Brasil. Ministério da Saúde. Resolução nº 135 de 08 de fevereiro de 2017. Regulamento técnico referente a alimentos para fins especiais. Diário Oficial da União. 2017 fev 08; seção 1. https://bvsms.saude.gov.br/bvs/saudelegis/anvisa/2017/rdc0135_08_02_2017.pdf

3. Brasil. Ministério da Agricultura, Pecuária e Abastecimento. Portaria SDA/MAPA nº 1.333, de 21 de julho de 2025. Regulamento Técnico de Identidade e Qualidade de Bebida Láctea. Diário Oficial da União. 2025 jul 23; seção 1. https://www.legisweb.com.br/legislacao/?id=481476

4. IBGE. Censo Agropecuário 2006 e 2017. Disponível em: https://sidra.ibge.gov.br

5. Cruz, A. G. et al. Química, bioquímica, análise sensorial e nutrição no processamento de leite e derivados. Rio de Janeiro: Elsevier, 2016.

6. Dayenoff P, Pizarro J, Banus G, Andrade-Montemayor H. Valoración de las fracciones de caseínas en leche de cabra Criolla y Saanen. Ciência Veteinária. 2021; 23(2): 77-90. http://dx.doi.org/10.19137/cienvet202123202

7. Pappa EC, Kondyli E, Bosnea L, Malamou E, Vlachou AM. Chemical,microbiological, sensory, and rheological properties of fresh goat milk cheese made by different starter cultures during storage. Journal of Food Process Engineering. 2022; 45(7),e13788. https://doi.org/10.1111/jfpe.13788

8. Costa MP, Frasao BS, Silva ACO, Freitas MQ, Franco RM, Conte-Junior CA. Cupuassu (Theobroma grandiflorum) pulp, probiotic, and prebiotic: Influence on color, apparent viscosity, and texture of goat milk yogurts. Journal of Dairy Science. 2015; 98(9), 5995–6003. https://doi.org/10.3168/jds.2015-9738

9. Siriwardhana J, Rasika DMD, Yapa D, Weerathilake WADV, Priyashantha, H. Enhancing probiotic survival and quality of fermented goat milk beverages with bael (Aegle marmelos) fruit pulp. Food Chemistry Advances. 2024; 5,100792 https://doi.org/10.1016/j.focha.2024.100792

10. El-Sayed MI, Aly E, El-Deeb AM. Improving the physicochemical and antioxidative properties of fermented goat milk using carob molasses and some probiotic strains. Food Science and Biotechnology. 2024; 33(3), 657–666. https://doi.org/10.1007/s10068-023-01382-2

11. Silva RV, Costa SC, Branco CR, Branco A. Invitro photoprotective activity of the Spondias purpurea L. peel crude extract and its incorporation in a pharmaceutical formulation. Industrial Crops and Products. 2016, 83: 509-514. http://doi.org/10.1016/j.indcrop.2015.12.077

12. Neris TS, Loss RA, Guedes, SF.Physical-chemical characterization of seriguela (Spondias purpurea L.) collected in the municipality of Barra do Bugres/MT at different maturation stages. Natural Resources. 2017, 7: 9-18 https://doi.org/10.6008/SPC2237-9290.2017.001.0002

13. Instituto Adolfo Lutz. Métodos físico químicos para análise de alimentos, 1ª ed. Digital: São Paulo, 2008. Disponível em: http://www.ial.sp.gov.br/resources/editorinplace/ial/2016_3_19/analisedealimentosial_2008.pdf

14. Okazaki M M, Nascimento MS, Taniwaki MH, Silveira NFA, Silva N da, Gomes RAR, Junqueira VCA. Métodos de análise microbiológica em alimentos. ITAL: Instituto de Tecnologia de Alimentos: São Paulo, 2015.

15. Ashraf R, Shah NP.Selective and differential enumerations of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium spp. in yoghurt — A review. International Journal of Food Microbiology. 2011, 149 (3):194-208. https://doi.org/10.1016/j.ijfoodmicro.2011.07.008

16. Brasil. Ministério da Agricultura, Pecuária e Abastecimento. Instrução Normativa MAPA nº 46 de 23/10/2007. Regulamento Técnico de Identidade e Qualidade de leites fermentados. Diário Oficial da União. 2007 out 24;seção 1. https://www.normasbrasil.com.br/norma/?id=76475

17. Costa AVS, Nicolau ES, Torres MCL, Fernandes PR, Rosa, SIR, Nascimento, RC. Desenvolvimento e caracterização físico-química, microbiológica de bebida láctea fermentada elaborada com diferentes estabilizantes/espessantes. Semina: Ciências Agrárias. 2013; 34(1):209-226. http://doi.org/10.5433/1679-0359

18. Gomes JJL, Duarte AM, Batista ASM, Figueiredo RMF, Sousa EP, Souza EL Physicochemical and sensory properties of fermented dairy beverages made with goat’s milk, cow’s milk and a mixture of the two milks. LWT-Food Science and Technology. 2013; 54 (1),18-24. https://doi.org/10.1016/j.lwt.2013.04.022

19. Vénica CI, Wolf IV, Bergamini, CV, Perotti M.C. Influence of lactose hydrolysis on galacto-oligosaccharides, lactose, volatile profile and physicochemical parameters of diferent yogurt varieties. Journal of the Science of Food and Agriculture. 2016; 96(15), 4929-4939. https://doi.org/10.1002/jsfa.7870

20. Nicolucia IG, Takehara CT, Bragotto APA. Edulcorantes de alta intensidade: tendências de uso em alimentos e avanços em técnicas analíticas. Química Nova. 2022, 45(2): 207-217. http://dx.doi.org/10.21577/0100-4042.20170828

21. Kant R. Sweet proteins – Potential replacement for artificial low calorie sweeteners. Nutritional Journal. 2005, 4(5): 1 – 6. https://doi.org/10.1186/1475-2891-4-5

22. Saraiva A, Carrascosa C, Raheem D, Ramos F, Raposo, A. Maltitol: Analytical Determination Methods, Applications in the Food Industry, Metabolism and Health Impacts.International Journal of Environmental Research and Public Health. 2020, 17: 1-28. https://doi.org/10.3390/ijerph17145227

23. Silva G de J., Gonçalves BRF, Conceição DG, Pontes SFO, Ferrão SPB. Perfil de ácidos graxos e frações proteícas do leite de cabra. Revista do Instituto Laticínios Cândido Tostes. 2015, 70 (6): 338-348. https://doi.org/10.14295/2238-6416.v70i6.474

24. Dayenoff P, Pizarro J, Banus G, Andrade-Montemayor H. Valoración de las fracciones de caseínas en leche de cabra Criolla y Saanen. Ciência Veterinária. 2021, 23 (2): 77-90. http://dx.doi.org/10.19137/cienvet202123202

25. Jesus MACL, Araujo RO, Oliveira CHS, Pereira IS. Processamento e análises físico química, microbiológica e sensorial de doce de leite pastoso sem adição de açúcar e formulado com soro de leite. Research, Society and Development. 2024, 13(8):v. 13, n.8, e8113846582. http://dx.doi.org/10.33448/rsd-v13i8.46582

26. Rodrigues, APS, Santos GAS, Carvalho HJM, Schmiele M, Sobrinho PSC, Rocha LOF.Efeito da adição de colágeno e sucralose nas características tecnológicas e sensoriais do iogurte. Research, Society and Development. 2021, 10 (11): e507101119841. http://doi.org/10.3390/nu11102494

27. Santana ATMC, Bachiega P, Morzelle MC, Abreu LR, Souza EC. Avaliação Sensorial de iogurte à base de pitaia (Hylocereus undatus), enriquecido com quinoa (Chenopodium quinoa) e sucralose. Revista Instituto Laticínios “Cândido Tostes”. 2012 (67): 21-25.

28. Silva AA, Souza PHM, Oliveira, LS, Gaban, SVF. Effects of Seriguela (Spondias purpurea) and Orange Peel (Citrus sinensis) on the Physicochemical and Sensory Properties, Phenolic Content, and Antioxidant Activity of Wheat Beer. Food Science and Technology. 2024 (4), 2867 – 2877 http://doi.org/10.1021/acsfoodscitech.4c00453

29. Nogueira JP, Jesus MACL. Desenvolvimento, avaliação físico-química, sensorial e colorimétrica da geleia de seriguela diet. Revista Brasileira de Tecnologia Agroindustrial. 2014 (8): 1531-1544 https://doi.org/10.3895/S1981-36862014000200007S1

30. Ranadheera CS, Naumovski N, Ajlouni S. Non-bovine milk products as emerging probiotic carriers: Recent developments and innovations. Current Opinion in Food Science. 2018; 22, 109–114. https://doi.org/10.1016/j.cofs.2018.02.010

31. El-Sayed MI, Aly E, El-Deeb AM. Improving the physicochemical and antioxidative properties of fermented goat milk using carob molasses and some probiotic strains. Food Science and Biotechnology. 2024; 33(3), 657–666. https://doi.org/10.1007/s10068-023-01382-2

32. Soares JVS, Costa ACP, Silva AKS, Moura RPM, Pereira CVL. Caracterização físico-química de iogurte grego probiótico de Lactobacillus acidophilus endocapsulados com calda de cubiu (Solanum sessiliflorum Dunal). Brazilian Journal of Development. 2023, 9 (3): 9139-9159. https://doi.org/10.34117/bjdv9n3-019

33. Machado TADG, de Oliveira MEG, Campos MIF, de Assis POA, de Souza EL, Madruga MS. Impact of honey on quality characteristics of goat yogurt containing probiotic Lactobacillus acidophilus. LWT - Food Science anda Technology. 2017; 80, 221–229. https://doi.org/10.1016/j.lwt.2017.02.013

34. Elkot WF, Talaat H, Abdeldaiem AM, Alnuzaili ES, Eljeam HAA, Ammar AF. Effect of using dried white sapote fruit (Casimiroa edulis)on the quality characteristics of bio-low-fat goat milk yoghurt drink. Saudi Journal of Biological Sciences. 2023; 30(12),103844. https://doi.org/10.1016/j.sjbs.2023.103844

35. Brasil. Instrução Normativa n° 161 de 01 de julho de 2022. Estabelece os padrões microbiológicos dos alimentos. Diário Oficial da União. 6 jul 2022;126 (Seção 1):235.

Published

2026-03-27

How to Cite

JESUS, Maria Antônia Carvalho Lima de; ALMEIDA, Lorraine Rodrigues de; CARDOSO, Letícia Alves; CARNEIRO, Maria Heloisa de Almeida; COSTA, Leonardo Oliveira; SILVA, Julia Carneiro da; SILVA, Leticia Cunha da; SANTOS, Iasmim da Silva. Probiotic and dietary fermented milk drink made from goat’s milk with added seriguela pulp: processing and analysis. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 27, 2026. Disponível em: https://revistas.ufg.br/vet/article/view/83889. Acesso em: 29 mar. 2026.

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Section

ANIMAL SCIENCE

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  • The research data is available on demand, condition justified in the manuscript