Antioxidant potential of pequi esters (Caryocar coriaceum) in bovine semen cryopreservation and in vitro fertilization
DOI:
https://doi.org/10.1590/1809-6891v26e-80138EAbstract
Bovine semen cryopreservation is a fundamental procedure in animal reproduction.
However, it is associated with the production of reactive oxygen species (ROS), which can cause
significant damage to spermatozoa, leading to infertility and reduced sperm quality. In this context,
using seminal diluents enriched with antioxidants presents a promising strategy for minimizing or even
reversing these harmful effects. This study aimed to evaluate the effects of different concentrations of
pequi (Caryocar coriaceum) esters as a supplement to bovine semen cryopreservation diluents. Fortytwo
ejaculates were collected from six bulls that had undergone an andrological examination and
were deemed suitable. The samples were analyzed, diluted in Tris–egg yolk medium, supplemented
with pequi esters (0.0, 0.5, 1.0, and 1.5 mL/L), packaged, and cryopreserved. The sperm parameters
evaluated in the post-thaw semen included the thermoresistance, plasma membrane functionality,
cleavage and blastocyst rates, and quantification of reduced glutathione and malondialdehyde. The
results showed that the concentrations of 1.0 and 1.5 mL/L of pequi esters significantly reduced
malondialdehyde levels in cryopreserved semen. However, no significant effects of pequi esters
were observed on motility parameters, sperm functionality, cleavage and blastocyst rates, or on
the quantification of reduced glutathione. Supplementation with pequi ester (1.0 and 1.5 mL/L) in
the cryopreservation of bovine semen may be beneficial in minimizing lipid peroxidation effects, as
evidenced by the reduction in malondialdehyde concentration after thawing.
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