ESTIMATION OF VOLUME OF EPITHELIAL LINING FLUID RECOVERED BY BRONCHOALVEOLAR LAVAGE IN EQUINE

Authors

  • Katia Moreira Silva Universidade Federal Fluminense
  • Carlos Frederico Marques Guimarães Universidade Federal Fluminense
  • Lara Medeiros Blasquez Olmedo Universidade Federal Fluminense
  • Estevão Grossi Aguiar Silva Exército Brasileiro
  • Nayro Xavier Alencar Universidade Federal Fluminense
  • Daniel Augusto Barroso Lessa Universidade Federal Fluminense

DOI:

https://doi.org/10.5216/cab.v11i3.5137

Keywords:

Animal Sanity

Abstract

Bronchoalveolar lavage (BAL) is a widespread technique that allows collection of pulmonary epithelial lining fluid (PELF) and evaluation of integrity of the lower airways. The instillation of isotonic solution needed for collection results in significant dilution of the   PELF. Due to its ability to freely cross biologic membranes urea is commonly used as a marker of PELF dilution. Nevertheless, the previously established methodology is difficult to be reproduced, especially since it utilizes reagents and equipments that are difficult to obtain. The objective of this study was to describe a methodology for measuring urea utilizing a commercial kit (Ureia UVLabTest®). Paired samples of blood and BALF were obtained from 18 adult horses. A standard curve of known urea dilutions in equine BALF provided a linear regression equation that allowed calculation of unknown urea concentrations in BALF samples. The mean estimated PELF volume was 0.52±0.12mL. The mean PELF percentage in relation to recovery BALF was 0.39±0.08%. These results are compatible with the ones previously described, demonstrating that the methodology presented here produced reliable results and can be utilized to estimate the PELF volume and consequently calculate concentration or activity of components or substances of interest.

KEY WORDS: Bronchoalveolar lavage, horses, pulmonary epithelial lining fluid, urea.

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Author Biographies

Katia Moreira Silva, Universidade Federal Fluminense

Médica veterinaria, mestranda do programa de pós graduação em clinica e reprodução da universidade federal fluminense, atuando principalmente na área de patologia clínica veterinaria e clinica de equinos, com enfase no aparelho respiratório

Carlos Frederico Marques Guimarães, Universidade Federal Fluminense

Químico, MSc, Departamento de Controle Químico de POA – Universidade Federal Fluminense

Lara Medeiros Blasquez Olmedo, Universidade Federal Fluminense

Médica Veterinária – Universidade Federal Fluminense

Estevão Grossi Aguiar Silva, Exército Brasileiro

Médico Veterinário, MSc, Exército Brasileiro.

Nayro Xavier Alencar, Universidade Federal Fluminense

Médico Veterinário, MSC, Dr.. Prof. Adjunto III – Patologia Clínica Veterinária – Universidade Federal Fluminense

Daniel Augusto Barroso Lessa, Universidade Federal Fluminense

Médico Veterinario, MSc. Dr. Prof. Adjunto III, Cínica Médica de Grandes Animais, UNiversidade Federal Fluminense

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Published

2010-10-01

How to Cite

SILVA, K. M.; GUIMARÃES, C. F. M.; OLMEDO, L. M. B.; SILVA, E. G. A.; ALENCAR, N. X.; LESSA, D. A. B. ESTIMATION OF VOLUME OF EPITHELIAL LINING FLUID RECOVERED BY BRONCHOALVEOLAR LAVAGE IN EQUINE. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 3, p. 576–581, 2010. DOI: 10.5216/cab.v11i3.5137. Disponível em: https://revistas.ufg.br/vet/article/view/5137. Acesso em: 24 nov. 2024.

Issue

Vol. 11 No. 3 (2010)

Section

Veterinary Medicine

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