Estimativa do volume de fluido epitelial pulmonar recuperado no lavado broncoalveolar no equino

Katia Moreira Silva; Carlos Frederico Marques Guimarães; Lara Medeiros Blasquez Olmedo; Estevão Grossi Aguiar Silva; Nayro Xavier Alencar; Daniel Augusto Barroso Lessa

doi:10.5216/cab.v11i3.5137

ESTIMATION OF VOLUME OF EPITHELIAL LINING FLUID RECOVERED BY BRONCHOALVEOLAR LAVAGE IN EQUINE

Authors

Katia Moreira Silva Universidade Federal Fluminense (UFF), Niterói, Rio de Janeiro, Brasil
Carlos Frederico Marques Guimarães Universidade Federal Fluminense (UFF), Niterói, Rio de Janeiro, Brasil
Lara Medeiros Blasquez Olmedo Universidade Federal Fluminense (UFF), Niterói, Rio de Janeiro, Brasil
Estevão Grossi Aguiar Silva Exército Brasileiro
Nayro Xavier Alencar Universidade Federal Fluminense (UFF), Niterói, Rio de Janeiro, Brasil
Daniel Augusto Barroso Lessa Universidade Federal Fluminense (UFF), Niterói, Rio de Janeiro, Brasil

DOI:

https://doi.org/10.5216/cab.v11i3.5137

Keywords:

Animal Sanity

Abstract

Bronchoalveolar lavage (BAL) is a widespread technique that allows collection of pulmonary epithelial lining fluid (PELF) and evaluation of integrity of the lower airways. The instillation of isotonic solution needed for collection results in significant dilution of the PELF. Due to its ability to freely cross biologic membranes urea is commonly used as a marker of PELF dilution. Nevertheless, the previously established methodology is difficult to be reproduced, especially since it utilizes reagents and equipments that are difficult to obtain. The objective of this study was to describe a methodology for measuring urea utilizing a commercial kit (Ureia UVLabTest®). Paired samples of blood and BALF were obtained from 18 adult horses. A standard curve of known urea dilutions in equine BALF provided a linear regression equation that allowed calculation of unknown urea concentrations in BALF samples. The mean estimated PELF volume was 0.52±0.12mL. The mean PELF percentage in relation to recovery BALF was 0.39±0.08%. These results are compatible with the ones previously described, demonstrating that the methodology presented here produced reliable results and can be utilized to estimate the PELF volume and consequently calculate concentration or activity of components or substances of interest.

KEY WORDS: Bronchoalveolar lavage, horses, pulmonary epithelial lining fluid, urea.

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Published

2010-10-01

How to Cite

SILVA, K. M.; GUIMARÃES, C. F. M.; OLMEDO, L. M. B.; SILVA, E. G. A.; ALENCAR, N. X.; LESSA, D. A. B. ESTIMATION OF VOLUME OF EPITHELIAL LINING FLUID RECOVERED BY BRONCHOALVEOLAR LAVAGE IN EQUINE. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 3, p. 576–581, 2010. DOI: 10.5216/cab.v11i3.5137. Disponível em: https://revistas.ufg.br/vet/article/view/5137. Acesso em: 12 apr. 2025.

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Issue

Vol. 11 No. 3 (2010)

Section

Veterinary Medicine

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