Antibiotic resistance gene occurrence in poultry farms in northeast Brazil

Henrique Francisco de Almeida; Paulo Ricardo Conceição Marques Trindade; César Roberto Viana Teixeira; Claudson Oliveira Brito; Silvio Santana  Dolabella; Sona  Jain; Maíra Pompeu  Martins; Ana Andréa Barbosa

doi:10.1590/1809-6891v26e-79298E

Autores

Henrique Francisco de Almeida Universidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, Brasil https://orcid.org/0000-0003-3825-9598
Paulo Ricardo Conceição Marques Trindade Universidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, Brasil https://orcid.org/0000-0001-8613-3784
César Roberto Viana Teixeira Universidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, Brasil https://orcid.org/0000-0002-2487-4314
Claudson Oliveira Brito Universidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, Brasil https://orcid.org/0000-0002-3339-8647
Silvio Santana Dolabella Universidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, Brasil https://orcid.org/0000-0001-5576-3214
Sona Jain Universidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, Brasil https://orcid.org/0000-0002-5999-931X
Maíra Pompeu Martins Universidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, Brasil https://orcid.org/0000-0002-9462-5145
Ana Andréa Barbosa Universidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, Brasil https://orcid.org/0000-0003-1271-7546

DOI:

https://doi.org/10.1590/1809-6891v26e-79298E

Resumo

O uso indevido de antibióticos na produção animal pode exercer pressão seletiva sobre cepas bacterianas, intensificando a disseminação de bactérias patogênicas e comensais portadoras de genes de resistência a antibióticos (GRAs). O objetivo deste estudo foi investigar a presença de GRAs em camas de frango provenientes de granjas avícolas localizadas no Estado de Sergipe, no Nordeste do Brasil. Um total de 14 amostras de cama de frango foram coletadas de doze fazendas e submetidas à extração de DNA total. A presença de GRAs foi testada por Reação em Cadeia da Polimerase (PCR) usando primers para principais classes de antibióticos. GRAs foram confirmados em todas as amostras, e a maior positividade para resistência foi obtida para tetraciclinas (tetA, tetM, and tetG), quinolonas (gyrA and qnrS), beta-lactâmicos (blaTEM), macrolídeos (ermB) e sulfonamidas (sul-1). O sequenciamento e a comparação com o banco de dados GenBank confirmaram a identidade dos GRAs. Algumas das sequências amplificadas por PCR eram semelhantes a fatores de resistência encontrados em bactérias Gram- positivo e Gramnegativo de diferentes espécies, principalmente enterobactérias. Além disso, foi observada semelhança para determinantes de resistência localizados tanto no cromossomo quanto em plasmídeos, transposons e integrons. Nossos resultados indicam o potencial da criação de aves para a disseminação ambiental de GRAs no Estado de Sergipe.

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