Cicatrização de feridas em coelhos com biomateriais autólogos associados à rosuvastatina

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Resumo

O plasma rico em plaquetas autólogo (PRPa) e a fibrina rica em plaquetas autóloga (FRPa) são biomateriais derivados do sangue com potencial promissor para uso na otimização do processo de cicatrização de feridas. A rosuvastatina (RSV), uma estatina hipolipemiante, apresenta efeitos pleiotrópicos que podem melhorar a cicatrização, justificando o estudo de seu uso isoladamente ou em combinação com outros materiais para tais fins. Este estudo teve como objetivo avaliar a reepitelização de feridas com o uso do PRPa e FRPa autólogos associados ou não à rosuvastatina 1,2%. Foram utilizados dezesseis coelhos machos adultos da raça Nova Zelândia, clinicamente saudáveis, alocados aleatoriamente em dois grupos de 8 animais, cada grupo representando um  biomaterial associado ou não à rosuvastatina 1,2%. As feridas cirúrgicas foram induzidas e tratadas com biomateriais e rosuvastatina 1,2% por 17 dias. Avaliação macroscópica da área total das feridas e a distância do epitélio em cortes histológicos foram realizadas. A correlação entre área de ferida e espessura de epitélio foi inversamente proporcional e com maior intensidade com o uso da FRPa (r = -0,5500). Não foi observada diferença na espessura de epitélio entre os grupos (p > 0,05). A área da ferida do PRPa isolado (p = 0,001), FRPa isolada (p = 0,021) e PRPa+RSV (p = 0,016) foram menores comparadas ao FRPa+RSV aos 14 dias. Esses achados sugerem que a rosuvastatina associada ao PRPa resultou em áreas menores de ferida comparada à PRFa, otimizando a cicatrização.

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Referências

Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature. 2008;453(7193):314-321. https://doi.org/10.1038/nature07039

Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, et al. Epithelialization in wound healing: a comprehensive review. Adv Wound Care. 2014;3(7):445-464. https://doi.org/10.1089/wound.2013.0473

Roy S, Driggs J, Elgharably H, Biswas S, Findley M, Khanna S, et al. Platelet‐rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation. Wound Repair Regen. 2011;19(6):753-766. https://doi.org/10.1111/j.1524-475X.2011.00740.x.

Hermeto LC, Rossi RD, Pádua SBD, Pontes ERJ, Santana AE. Comparative study between fibrin glue and platelet rich plasma in dogs skin grafts. Acta Cir Bras. 2012;27:789-794. https://doi.org/10.1590/S0102-86502012001100008

Desai CB, Mahindra UR, Kini YK, Bakshi MK. Use of platelet-rich fibrin over skin wounds: Modified secondary intention healing. J Cutan Aesthet Surg. 2013;6(1):35. https://doi.org/10.4103/0974-2077.110096

Ehrenfest DMD, Andia I, Zumstein MA, Zhang CQ, Pinto NR, Bielecki T. Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives. Muscles Ligaments Tendons J. 2014;4(1):3. (https://pmc.ncbi.nlm.nih.gov/articles/PMC4049647/)

Prakash S, Thakur A. Platelet concentrates: past, present and future. J Maxillofac Oral Surg. 2011;10(1):45-49. https://doi.org/10.1007/s12663-011-0182-4

Sanchez-González DJ, Méndez-Bolaina E, Trejo-Bahena NI. Platelet-rich plasma peptides: key for regeneration. Int J Pept. 2012;2012:532519. https://doi.org/10.1155/2012/532519

Barrionuevo DV, Laposy CB, Abegão KGB, Nogueira RMB, Nai GA, Bracale BN, et al. Comparison of experimentally-induced wounds in rabbits treated with different sources of platelet-rich plasma. Lab Anim. 2015;49(3):209-214. https://doi.org/10.1177/0023677214567747

Ferreira NGO, Vicentini YF, Breda MRS, Nogueira RMB, Nai GA, Santarém CL. Uso de biomateriais e rosuvastatina tópica aumenta angiogênese de feridas cirúrgicas em coelhos. Res Soc Dev. 2021;10(1). https://doi.org/10.33448/rsd-v10i1.11327

Bao JW, Sun B, Ma PP, Gai YS, Sun WZ, Yu HQ, et al. Rosuvastatin inhibits inflammatory response and resists fibrosis after myocardial infarction. Eur Rev Med Pharmacol Sci. 2018;22(1). https://doi.org/10.26355/eurrev_201801_14123

Pradeep AR, Karvekar S, Nagpal K, Patnaik K, Guruprasad CN, Kumaraswamy KM. Efficacy of locally delivered 1.2% rosuvastatin gel to non‐surgical treatment of patients with chronic periodontitis: A randomized, placebo‐controlled clinical trial. J Periodontol. 2015;86(6):738-745. https://doi.org/10.1902/jop.2015.140631

Asai J, Takenaka H, Hirakawa S, Sakabe JI, Hagura A, Kishimoto S, et al. Topical simvastatin accelerates wound healing in diabetes by enhancing angiogenesis and lymphangiogenesis. Am J Pathol. 2012;181(6):2217-2224. https://doi.org/10.1016/j.ajpath.2012.08.023

Salem, H.F.; Nafady, M.M.; Ewees, M.G.E.-D.; Hassan, H.; Khallaf, R.A. Rosuvastatin calcium-based novel nanocubic vesicles capped with silver nanoparticles-loaded hydrogel for wound healing management: Optimization employing Box–Behnken design: In vitro and in vivo assessment. J. Liposome Res. 2022, 32, 45–61. https://doi.org/10.1080/08982104.2020.1867166

Aly, U.F.; Abou-Taleb, H.A.; Abdellatif, A.A.; Tolba, N.S. Formulation and evaluation of simvastatin polymeric nanoparticles loaded in hydrogel for optimum wound healing purpose. Drug Des. Dev. Ther. 2019, 13, 1567–1580. https://doi.org/10.2147/DDDT.S198413

Diretrizes da prática de eutanásia do CONCEA (2013). In: Ministério Da Ciência, Tecnologia e Inovação, Conselho Nacional de Controle de Experimentação Animal – CONCEA. https://antigo.mctic.gov.br/mctic/opencms/legislacao/outros_atos/resolucoes/Resolucao_CONCEA_n_37_de_15022018.html

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals: Environment, housing and management. 8th ed. The National Academic Press. https://doi.org/10.17226/12910

Percie du Sert N, Hurst V, Ahluwalia A, Alam S, Avey MT, Baker M, et al. The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. PLoS Biol. 2020;18(7):e3000410. https://doi.org/10.1371/journal.pbio.3000410

Grover HS, Kapoor S, Singh A. Effect of topical simvastatin (1,2 mg) on gingival crevicular fluid interleukin-6, interleukin-8 and interleukin-10 levels in chronic periodontitis- a clinic biochemical study. J Oral Biol Craniofac Res. 2016;6(2):85-92. https://doi.org/10.1016/j.jobcr.2015.11.003

Abegão KGB, Bracale BN, Delfim IG, Santos ESD, Laposy CB, Nai GA, et al. Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits. Acta Cir Bras. 2015;30:209-215. https://doi.org/10.1590/S0102-865020150030000008

Taniguchi BA, Breda MRS, Nogueira RMB, Nai GA, Laposy CB. Fractal features of rabbit dermal wounds treated with platelet-rich plasma and topical rosuvastatin. Int J Clin Exp Pathol. 2018;11(11):5241. (https://pmc.ncbi.nlm.nih.gov/articles/PMC6963036/)

Tetila AF, Breda MRS, Nogueira RMB, Nai GA, Laposy CB. The use of platelet-rich plasma and rosuvastatin in wound healing in rabbits: a longitudinal study. Adv Skin Wound Care. 2019;32(9):1-5. https://doi.org/10.1097/01.ASW.0000577136.88748.68

Azevedo, M.C.M.P.S. Aplicação do PRF em medicina dentária. 2014. 29f. 51. Dissertação (Mestrado Integrado em Medicina Dentária) – Faculdade de Medicina Dentária, Universidade do Porto – Portugal. 2014. (https://hdl.handle.net/10216/75440)

Kanashiro GP, Cassu RN. Anestesia em animais selvagens e de laboratório. In: Andrade SF. Manual de terapêutica veterinária. 3rd ed. Roca; 2008. pp.728-745.

Vendramin FS, Franco D, Schamall RF, Franco TR. Utilização do plasma rico em plaquetas autólogo em enxertos cutâneos em coelho. Rev Bras Cir Plástica. 2010;25:4-10. (https://www.rbcp.org.br/details/549/pt-BR)

Carvalho V, Gomes FSL, Carmo DJ, Batista JA, Viana MN. Planimetria como método para mensuração de feridas. Rev Mineira Enferm. 2006;10(4):425-428. https://doi.org/10.35699/reme.v10i4.50743

Sun Y, Cao Y, Zhao R, Xu F, Wu D, Wang Y. The role of autologous APRP on deep partial-thickness burn wound healing in bama pigs. J Burn Care Res. 2020;41(3):657-662. https://doi.org/10.1093/jbcr/iraa012

Xu P, Wu Y, Zhou L, Yang Z, Zhang X, Hu X, et al. Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization. Burns Trauma. 2020;8: tkaa028. https://doi.org/10.1093/burnst/tkaa028.

Kobayashi E, et al. Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Investig. 2016;20:2353-60. https://doi.org/10.1007/s00784-016-1719-1

Khalifa OA, Elkasapy A, Sallam EA, Marei YM, Mohammed LS. Effect of Autologous platelet-rich plasma versus platelet-rich fibrin on the Second Intension Wound Healing in Dogs through higher regeneration capacity and modulation of inflammatory cytokines. Benha Vet Med J. 2021;41(1):1-7. https://doi.org/10.21608/BVMJ.2021.92616.1465

Snowden JM. Wound closure: an analysis of the relative contributions of contraction and epithelialization. J Surg Res. 1984;37(6):453-463. https://doi.org/10.1016/0022-4804(84)90213-0

Bull RH, Staines KL, Collarte AJ, Bain DS, Ivins NM, Harding KG. Measuring progress to healing: A challenge and an opportunity. Int Wound J. 2022;19(4):734-740. https://doi.org/0.1111/iwj.13669

Balse NS, Baliga S. “Evaluation of wound healing and bone regeneration using autologous platelet-rich plasma and platelet-rich fibrin postextractions”: A comparative study. Indian J Health Sci Biomed Res KLEU. 2017;10(2):167-172. https://doi.org/10.4103/kleuhsj.ijhs_395_16

Yerke LM, Jamjoom A, Zahid TM, Cohen RE. The effect of platelet-rich fibrin, calcium sulfate hemihydrate, platelet-rich plasma and resorbable collagen on soft tissue closure of extraction sites. J Funct Biomater. 2017;8(2):17. https://doi.org/10.3390/jfb8020017

Khalaf FH, Salih SI. Clinical and histopathological evaluation of using platelet-rich plasma and platelet-rich fibrin matrix in treatment of induced chronic open wounds in bucks. Asian J Pharm Clin Res. 2018;11(5):337-341. https://doi.org/10.22159/ajpcr.2018.v11i5.24105

Kim TH, Kim SH, Sándor GK, Kim YD. Comparison of platelet-rich plasma (APRP), platelet-rich fibrin (APRF), and concentrated growth factor (CGF) in rabbit-skull defect healing. Arch Oral Biol. 2014;59(5):550-558. https://doi.org/10.1016/j.archoralbio.2014.02.004

Bansal S, Garg A, Khurana R, Chhabra P. Platelet-rich fibrin or platelet-rich plasma–which one is better? an opinion. Indian J Dent Sci. 2017;9(Suppl 1):S49-S52. https://doi.org/10.4103/IJDS.IJDS_55_17

Xiong S, Qiu L, Su Y, Zheng H, Yi C. Platelet-rich plasma and platelet-rich fibrin enhance the outcomes of fat grafting: a comparative study. Plast Reconstr Surg. 2019;143(6):1201e-1212e. https://doi.org/10.1097/PRS.0000000000005676

Pradeep AR, Karvekar S, Nagpal K, Patnaik K, Raju A, Singh P. Rosuvastatin 1.2 mg in situ gel combined with 1: 1 mixture of autologous platelet‐rich fibrin and porous hydroxyapatite bone graft in surgical treatment of mandibular class II furcation defects: A randomized clinical control trial. J Periodontol. 2016;87(1):5-13. https://doi.org/10.1902/jop.2015.150131

Gautam K, Kapoor A, Mathur S, Ali AR, Choudhary A, Shekhawat A. Comparative evaluation of autogenous bone graft and autologous platelet-rich fibrin with and without 1.2 mg in situ rosuvastatin gel in the surgical treatment of intrabony defect in chronic periodontitis patients. Contemp Clin Dent. 2022;13(1):69. https://doi.org/10.4103/ccd.ccd_740_20

Publicado

2025-02-05

Como Citar

VICENTINI, Y. F.; NAI, G. A.; NOGUEIRA, R. M. B.; SANTARÉM, C. . L. Cicatrização de feridas em coelhos com biomateriais autólogos associados à rosuvastatina. Ciência Animal Brasileira / Brazilian Animal Science, Goiânia, v. 26, 2025. Disponível em: https://revistas.ufg.br/vet/article/view/78752. Acesso em: 8 fev. 2025.

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MEDICINA VETERINÁRIA