Effects of Isomix® and virginiamycin on in vitro ruminal fermentation

Sara Adriana Salinas Laura; Rogério de Paula Lana; Márcia das Neves Soares; Paulo Sérgio Dornelas; Ruth Ccalta Hancco

doi:10.1590/1809-6891v26e-81135E

Authors

Sara Adriana Salinas Laura Universidade Federal de Lavras (UFLA), Lavras, Minas Gerais, Brazil https://orcid.org/0000-0001-6569-3344
Rogério de Paula Lana Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil https://orcid.org/0000-0001-5200-9005
Márcia das Neves Soares Universidade Federal de Lavras (UFLA), Lavras, Minas Gerais, Brazil https://orcid.org/0000-0001-9099-4181
Paulo Sérgio Dornelas Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil https://orcid.org/0000-0003-2033-7746
Ruth Ccalta Hancco Universidade Federal de Lavras (UFLA), Lavras, Minas Gerais, Brazil https://orcid.org/0000-0002-3730-4468

DOI:

https://doi.org/10.1590/1809-6891v26e-81135E

Abstract

Additives improve cattle feed efficiency by altering microbial populations. This study evaluated the effects of varying doses and the interaction between two additives on in vitro dry matter digestibility (IVDMD), neutral detergent fiber digestibility (IVNDFD), and ruminal fermentation parameters using rumen fluid from fistulated cows. Diets with a 70:30 forage-to-concentrate ratio were incubated for 48 h using the Tilley and Terry method, with Brachiaria decumbens as the forage and a concentrate composed of corn meal, soybean meal, and urea. The additives tested were Isomix® (from 0.0 to 4.8 % of diet dry matter) and virginiamycin (from 0.0 to 0.80 %). The significant effects (P<0.05) of virginiamycin included increased pH, NH3, and soluble protein (SP) and decreased IVDMD, IVNDFD, microbial protein (MP), and volatile fatty acids (VFA). Isomix had no significant effects on these variables, but increased isobutyrate concentration (P < 0.05). Additive interactions affected acetate, propionate, butyrate, isovalerate, and the acetate:propionate ratio (P<0.05). While neither additive improved digestibility, Isomix enhanced propionate production and reduced the acetate:propionate ratio, potentially improving energy balance and performance. Further research is recommended, particularly on diets with low protein and urea supplementation in tropical grazing systems.
Keywords: Branched-chain volatile fatty acids; in vitro digestibility; tropical pastures.

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