IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS

Authors

  • Romão da Cunha Nunes Universidade Federal de Goiás, Goiânia, Goiás, Brasil, romaocnunes@hotmail.com
  • Laritza Ferreira de Lima Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, laritza_lima@yahoo.com.br http://orcid.org/0000-0001-6391-7113
  • Rebeca Magalhães Pedrosa Rocha Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, rebeca_mpr@yahoo.com.br
  • Letícia Oliveira Universidade Federal de Goiás, Goiânia, Goiás, Brasil, leticiameloo@hotmail.com
  • Claudio Cabral Campello Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, cccampello@yahoo.com.br
  • Jose Ricardo de Figueiredo Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, figueiredo.lamofopa@gmail.com

DOI:

https://doi.org/10.1590/cab19048658

Abstract

This study investigated the effect of homeopathic medicine (Pulsatilla nigricans and homeopathic follicle stimulating hormone - FSH) and the complex (Bos Stress Fertilis) on the initial folliculogenesis, using the in vitro culture of preantral follicles as in vitro model. For this, swine ovarian fragments were cultured for one or seven days in ?-MEM+ in the absence (cultured control) or presence of homeopathic FSH (6 cH), Pulsatilla (6 CH), Bos Stress Fertilis (6 CH), grain alcohol (50% v / v) or recombinant FSH (50 ng / ml) added daily. Uncultured ovarian fragments (fresh control) or cultured for one and seven days were processed for classical histology. Only the homeopathic complex Bos Stress Fertilis maintained the percentage of follicular survival after seven days of culture in relation to the uncultured control and ?-MEM+. Regarding follicular growth, only the addition of homeopathic FSH increased the follicular diameter when compared to the uncultured control and a-MEM+. Thus, it can be concluded that the addition of the homeopathic remedies Bos Stress fertilis and homeopathic FSH (6 CH) improved, respectively, survival and in vitro growth of swine preantral follicles included in ovarian tissue.
Keywords: FSH; Homeopathy; Ovary; Preantral follicle; Swine

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Author Biographies

Romão da Cunha Nunes, Universidade Federal de Goiás, Goiânia, Goiás, Brasil, romaocnunes@hotmail.com

 http://lattes.cnpq.br/4335133347523404

Laritza Ferreira de Lima, Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, laritza_lima@yahoo.com.br

http://lattes.cnpq.br/3352238641564662

Rebeca Magalhães Pedrosa Rocha, Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, rebeca_mpr@yahoo.com.br

http://lattes.cnpq.br/4060982214181976

Claudio Cabral Campello, Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, cccampello@yahoo.com.br

http://lattes.cnpq.br/7450299703011981

Jose Ricardo de Figueiredo, Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, figueiredo.lamofopa@gmail.com

http://lattes.cnpq.br/5153504967210091

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Published

2018-08-02

How to Cite

DA CUNHA NUNES, R.; FERREIRA DE LIMA, L.; MAGALHÃES PEDROSA ROCHA, R.; OLIVEIRA, L.; CABRAL CAMPELLO, C.; RICARDO DE FIGUEIREDO, J. IN SITU CULTURED PREANTRAL FOLLICLES IS A USEFUL MODEL TO EVALUATE THE EFFECT OF HOMEOPATHY MEDICINE ON EARLY FOLLICULOGENESIS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 19, p. 1–8, 2018. DOI: 10.1590/cab19048658. Disponível em: https://revistas.ufg.br/vet/article/view/e-48658. Acesso em: 22 nov. 2024.

Issue

Vol. 19 (2018): Continuous publication

Section

MEDICINA VETERINÁRIA

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