SUSCEPTIBILITY OF Staphylococcus aureus ISOLATED FROM RAW MILK TO COMMERCIAL ANTIBIOTICS

Authors

  • Aparecida Selsiane Sousa Carvalho Universidade Estadual do Norte Fluminense, Campos de Goytacazes, Rio de Janeiro, Brasil, selsiane@hotmail.com
  • Josilene Lima Lima Serra Instituto Federal do Maranhão, São Luís, Maranhão, Brasil, josilene.serra@ifma.edu.br
  • Lucélia da Cunha Rodrigues Instituto Federal do Maranhão, São Luís, Maranhão, Brasil, lucelia.rodrigues@ifma.edu.br
  • Luciano de Souza Rodrigues Junior Instituto Federal do Maranhão, São Luís, Maranhão, Brasil, luciano.junior@ifma.edu.br
  • Adenilde Nascimento Mouchrek Universidade Federal do Maranhão, Barreirinhas, Maranhão, Brasil, adenild@bol.com.br
  • Elka Machado Ferreira Universidade Estadual Paulista, Jaboticabal, São Paulo, Brasil, elka.ferreira@hotmail.com

DOI:

https://doi.org/10.1590/cab19047159

Abstract

Milk is extremelly valuable to the human diet and an excellent substrate for the growth of a wide range of microorganisms, including Staphylococcus aureus, frequently found in raw milk and one of the most prevalent microorganisms in cases of bovine mastitis in the world. The objective of this study was to evaluate the sensitivity of S. aureus strains isolated from raw milk to commercial antimicrobials. For this end, antibiotics based on research were selected from commercial establishments of veterinary drugs in the municipality of Zé Doca (Maranhão State, Brazil). The antibiotics were identified by association with penicillin, streptomycin, isoniazid and prednisolone (PCB), oxytetracycline base (TOR), a combination of penicillin and streptomycin (MPP), and tetracycline hydrochloride (TCA). Subsequently, Staphylococcus spp. counting was perfomed, with isolation and biochemical identification of strains of S. aureus, which were then submitted to antibiograms with identification of beta-lactamase producing strains. The results showed contamination in all samples by Staphylococcus spp., with counts ranging from 1.9 x 103 to 5.24 x 106 CFU/mL. Among the antibiotics tested, TOR showed the highest efficiency in elimination or reduction of all S. aureus strains. We found that 50% of S. aureus strains isolated produced the enzyme beta-lactamase. It is necessary to create measures to combat the emergence of new bacterial strains and to promote action by public authorities, supervising the commercialization of veterinary drugs.
Keywords: Bacterial resistance; Beta-lactamase; Dairy products; Milk quality

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Author Biographies

Aparecida Selsiane Sousa Carvalho, Universidade Estadual do Norte Fluminense, Campos de Goytacazes, Rio de Janeiro, Brasil, selsiane@hotmail.com

http://lattes.cnpq.br/7100718471924333

Josilene Lima Lima Serra, Instituto Federal do Maranhão, São Luís, Maranhão, Brasil, josilene.serra@ifma.edu.br

http://lattes.cnpq.br/8413662880147518

Lucélia da Cunha Rodrigues, Instituto Federal do Maranhão, São Luís, Maranhão, Brasil, lucelia.rodrigues@ifma.edu.br

http://lattes.cnpq.br/6016263725018788

Luciano de Souza Rodrigues Junior, Instituto Federal do Maranhão, São Luís, Maranhão, Brasil, luciano.junior@ifma.edu.br

http://lattes.cnpq.br/0048825085382663

Adenilde Nascimento Mouchrek, Universidade Federal do Maranhão, Barreirinhas, Maranhão, Brasil, adenild@bol.com.br

http://lattes.cnpq.br/0141868231910835

Elka Machado Ferreira, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brasil, elka.ferreira@hotmail.com

 http://lattes.cnpq.br/9425029404862685

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Published

2018-10-02

How to Cite

SOUSA CARVALHO, A. S.; LIMA SERRA, J. L.; DA CUNHA RODRIGUES, L.; DE SOUZA RODRIGUES JUNIOR, L.; NASCIMENTO MOUCHREK, A.; MACHADO FERREIRA, E. SUSCEPTIBILITY OF Staphylococcus aureus ISOLATED FROM RAW MILK TO COMMERCIAL ANTIBIOTICS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 19, p. 1–8, 2018. DOI: 10.1590/cab19047159. Disponível em: https://revistas.ufg.br/vet/article/view/e-47159. Acesso em: 17 jul. 2024.

Issue

Vol. 19 (2018): Continuous publication

Section

MEDICINA VETERINÁRIA

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