INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION

Authors

  • Vanessa Haach Universidade Federal do Rio Grande do Sul, Instituto de Ciências Básicas da Saúde, Porto Alegre, Rio Grande do Sul, Brasil, vanessahaach@hotmail.com
  • Danielle Gava Empresa Brasileira de Pesquisa Agropecuária, Suínos e Aves (EMBRAPA), Concórdia, Santa Catarina, Brasil, danielle.gava@embrapa.br http://orcid.org/0000-0002-1917-2633
  • Arlei Coldebella Empresa Brasileira de Pesquisa Agropecuária, Suínos e Aves (EMBRAPA), Concórdia, Santa Catarina, Brasil, arlei.coldebella@embrapa.br
  • Rejane Schaefer Empresa Brasileira de Pesquisa Agropecuária, Suínos e Aves (EMBRAPA), Concórdia, Santa Catarina, Brasil, rejane.schaefer@embrapa.br

DOI:

https://doi.org/10.1590/cab19046789

Abstract

Influenza virus infection in pigs causes significant losses for the swine industry and concerns for the public health. Therefore, rapid virus detection is important for influenza surveillance in pigs. Storage conditions (such as medium, temperature, and time) of the biological samples are very important for the diagnosis because they can negatively interfere with the viral detection. In this study, influenza virus viability was evaluated in different storage media (UTM commercial medium, "in house" VTM medium, and without storage medium), temperature (4, 23 and 37 °C), and storage time (up to 120 hours). Samples were evaluated by RT-qPCR and isolation in embryonated chicken eggs. Significant effects (p<0.05) were observed for the media and time besides the interaction between the two factors with the viral load. In conclusion, biological samples of pigs sent for influenza diagnosis should be stored, preferably in viral maintenance medium at 4 °C, and the time estimated between the sample collection until the arrival in the laboratory should be less than three days.
Keywords: Diagnosis; Influenza; Swine; Viral viability

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Author Biographies

Vanessa Haach, Universidade Federal do Rio Grande do Sul, Instituto de Ciências Básicas da Saúde, Porto Alegre, Rio Grande do Sul, Brasil, vanessahaach@hotmail.com

http://lattes.cnpq.br/6703603450614548

Danielle Gava, Empresa Brasileira de Pesquisa Agropecuária, Suínos e Aves (EMBRAPA), Concórdia, Santa Catarina, Brasil, danielle.gava@embrapa.br

http://lattes.cnpq.br/9235786021845819

Arlei Coldebella, Empresa Brasileira de Pesquisa Agropecuária, Suínos e Aves (EMBRAPA), Concórdia, Santa Catarina, Brasil, arlei.coldebella@embrapa.br

 http://lattes.cnpq.br/0413169414195839

Rejane Schaefer, Empresa Brasileira de Pesquisa Agropecuária, Suínos e Aves (EMBRAPA), Concórdia, Santa Catarina, Brasil, rejane.schaefer@embrapa.br

http://lattes.cnpq.br/5155040243608278

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Published

2018-07-04

How to Cite

HAACH, V.; GAVA, D.; COLDEBELLA, A.; SCHAEFER, R. INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 19, p. 1–9, 2018. DOI: 10.1590/cab19046789. Disponível em: https://revistas.ufg.br/vet/article/view/e-46789. Acesso em: 22 nov. 2024.

Issue

Vol. 19 (2018): Continuous publication

Section

MEDICINA VETERINÁRIA

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