Adição de hidroxitolueno butilado (BHT) no diluidor ACP-106c para congelação de sêmen canino

Herlon Victor Rodrigues Silva; Thalles Gothardo Pereira Nunes; Antônio Cavalcante Mota Filho; José Nicodemos Pinto; Alexandre Rodrigues Silva; Lúcia Daniel Machado da Silva

doi:10.1590/1809-6891v19e-45896

ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING

Authors

Herlon Victor Rodrigues Silva Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil
Thalles Gothardo Pereira Nunes Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil
Antônio Cavalcante Mota Filho Faculdades Terra-Nordeste, Caucaia, Ceará, Brasil
José Nicodemos Pinto Médico Veterinário Autônomo, Fortaleza, Ceará, Brasil
Alexandre Rodrigues Silva Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brasil
Lúcia Daniel Machado da Silva Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil

DOI:

https://doi.org/10.1590/1809-6891v19e-45896

Keywords:

Animal Reproduction

Abstract

This study was conducted to determine the effect of butylated hydroxytoluene (BHT) on the quality of canine sperm frozen and thawed using the powdered coconut water based (ACP-106c) extender. Therefore, fifteen ejaculates were collected from five dogs. Semen obtained was diluted in ACP-106c added of glycerol and egg yolk. The samples were then transferred to tubes containing different concentrations of BHT (0, 0.5, 1.0 and 2.0 mM). After that, the samples were filled into straws, frozen and stored in liquid nitrogen. Fresh semen was evaluated for the following parameters: color, sperm fraction volume, total motility, vigor, concentration, morphology, and HOST test. After one week, the samples were thawed and evaluated by computer analysis, as well as for membrane functionality and sperm morphology. Progressive motility in the 2.0 mM BHT group was significantly higher (P <0.05) than that of the 0 mM BHT group (27.6 ± 11.7% vs. 19.0 ± 9.5%, respectively). Regarding all other parameters evaluated, there was no difference between the groups tested. Therefore, the addition of BHT to the ACP-106c extender did not affect the quality of canine semen after thawing.
Keywords: antioxidants; cryopreservation; dog; extenders.

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Published

2018-07-04

How to Cite

SILVA, Herlon Victor Rodrigues; NUNES, Thalles Gothardo Pereira; FILHO, Antônio Cavalcante Mota; PINTO, José Nicodemos; SILVA, Alexandre Rodrigues; SILVA, Lúcia Daniel Machado da. ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 19, p. 1–9, 2018. DOI: 10.1590/1809-6891v19e-45896. Disponível em: https://revistas.ufg.br/vet/article/view/e-45789. Acesso em: 6 dec. 2025.

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Issue

Vol. 19 (2018): Continuous publication

Section

ANIMAL SCIENCE

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