ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING

Authors

  • Herlon Victor Rodrigues Silva Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, herlonvrs@hotmail.com
  • Thalles Gothardo Pereira Nunes Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, thalles_gothardo@hotmail.com
  • Antônio Cavalcante Mota Filho Faculdades Terra-Nordeste, Caucaia, Ceará, Brasil, acmfnedvet@yahoo.com.br
  • José Nicodemos Pinto
  • Alexandre Rodrigues Silva Universidade Federal Rural do Semi-Árido, Mossoró, Rio Grande do Norte, Brasil, legio2000@yahoo.com
  • Lúcia Daniel Machado da Silva Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, lucia.daniel.machado@hotmail.com

DOI:

https://doi.org/10.1590/cab19045896

Keywords:

Animal Reproduction

Abstract

This study was conducted to determine the effect of butylated hydroxytoluene (BHT) on the quality of canine sperm frozen and thawed using the powdered coconut water based (ACP-106c) extender. Therefore, fifteen ejaculates were collected from five dogs. Semen obtained was diluted in ACP-106c added of glycerol and egg yolk. The samples were then transferred to tubes containing different concentrations of BHT (0, 0.5, 1.0 and 2.0 mM). After that, the samples were filled into straws, frozen and stored in liquid nitrogen. Fresh semen was evaluated for the following parameters: color, sperm fraction volume, total motility, vigor, concentration, morphology, and HOST test. After one week, the samples were thawed and evaluated by computer analysis, as well as for membrane functionality and sperm morphology. Progressive motility in the 2.0 mM BHT group was significantly higher (P <0.05) than that of the 0 mM BHT group (27.6 ± 11.7% vs. 19.0 ± 9.5%, respectively). Regarding all other parameters evaluated, there was no difference between the groups tested. Therefore, the addition of BHT to the ACP-106c extender did not affect the quality of canine semen after thawing.
Keywords: antioxidants; cryopreservation; dog; extenders.

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Author Biographies

Herlon Victor Rodrigues Silva, Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, herlonvrs@hotmail.com

http://lattes.cnpq.br/2748407705914248

Thalles Gothardo Pereira Nunes, Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, thalles_gothardo@hotmail.com

http://lattes.cnpq.br/7215138114783850

Antônio Cavalcante Mota Filho, Faculdades Terra-Nordeste, Caucaia, Ceará, Brasil, acmfnedvet@yahoo.com.br

http://lattes.cnpq.br/0928363834921017

José Nicodemos Pinto

http://lattes.cnpq.br/8729766606205419

Alexandre Rodrigues Silva, Universidade Federal Rural do Semi-Árido, Mossoró, Rio Grande do Norte, Brasil, legio2000@yahoo.com

http://lattes.cnpq.br/1959482950237684

Lúcia Daniel Machado da Silva, Universidade Estadual do Ceará, Fortaleza, Ceará, Brasil, lucia.daniel.machado@hotmail.com

http://lattes.cnpq.br/3610262531647532

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Published

2018-07-04

How to Cite

VICTOR RODRIGUES SILVA, H.; GOTHARDO PEREIRA NUNES, T.; CAVALCANTE MOTA FILHO, A.; NICODEMOS PINTO, J.; RODRIGUES SILVA, A.; DANIEL MACHADO DA SILVA, L. ADDITION OF BUTYLATED HYDROXY TOLUENE (BHT) TO THE ACP-106c EXTENDER FOR CANINE SEMEN FREEZING. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 19, p. 1–9, 2018. DOI: 10.1590/cab19045896. Disponível em: https://revistas.ufg.br/vet/article/view/e-45789. Acesso em: 17 jul. 2024.

Issue

Vol. 19 (2018): Continuous publication

Section

ZOOTECNIA

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