SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION

Authors

  • Daianny Barboza Guimarães Universidade Estadual do Ceará, Faculdade de Veterinária, Fortaleza, Ceará, Brasil, daiannybg@yahoo.com.br
  • Tatyane Bandeira Barros Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Instituto de Ciências Exatas e da Natureza, Redenção, Ceará, Brasil, tatybiobandeira@yahoo.com.br
  • Ludymila Furtado Cantanhêde Universidade Estadual do Ceará, Faculdade de Veterinária, Fortaleza, Ceará, Brasil, lud_furtado@hotmail.com
  • Jean Magloire Nguekam Feugang Universidade Estadual do Mississipi, Starkville, Mississippi, Estados Unidos, jn181@ads.msstate.edu
  • Leonardo Peres de Souza Universidade Federal do Piauí, Parnaíba, Piauí, Brasil, souza_l_p@yahoo.com.br
  • Ricardo Toniolli Universidade Estadual do Ceará, Faculdade de Veterinária, Departamento de Medicina Veterinária, Fortaleza, Ceará, Brasil, ricardo.toniolli@uece.br

DOI:

https://doi.org/10.1590/cab19138250

Keywords:

Sêmen suíno, Diluente alternative, Congelação, Viabilidade espermática

Abstract

Semen cryopreservation is associated with low productivity results. This study aimed to test the Coconut Water Powder (ACP®-103) as a ressuspension diluent after thawing semen, also evaluate sperm quality during the cooling curve until thawing of the semen. For this, the semen was collected from 15 boars once a week, incubated at 30 °C for 15 minutes, and afterwards, the samples were diluted in the diluent Beltsville Thawing Solution – BTS (Control) or ACP-103®, and subjected to a slow cooling curve, where the force and the motility were analyzed in each step. The thawed semen was ressuspended in its respective solvents and analyzed in the characteristics of vigor, motility, vitality, acrosome integrity and functionality of the membrane. During the analysis of vigor and motility that make up the cooling curve, and thawing, for analysis of vitality and intact acrosomal membrane, it was observed that there was no significant difference between treatments. Besides, after thawing, the BTS showed better results of sperm vigor, sperm motility and membrane functionality. However, the cooling curve and coconut water powder can be used in the protocol for cryopreservation of boar semen, as both ensured quality of sperm viability that may be used in artificial insemination.
Keywords: Swine semen; alternative extender; freezing; sperm viability.

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Author Biographies

Daianny Barboza Guimarães, Universidade Estadual do Ceará, Faculdade de Veterinária, Fortaleza, Ceará, Brasil, daiannybg@yahoo.com.br

 http://lattes.cnpq.br/3056895397727551

Tatyane Bandeira Barros, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Instituto de Ciências Exatas e da Natureza, Redenção, Ceará, Brasil, tatybiobandeira@yahoo.com.br

 http://lattes.cnpq.br/7223669960713191

Ludymila Furtado Cantanhêde, Universidade Estadual do Ceará, Faculdade de Veterinária, Fortaleza, Ceará, Brasil, lud_furtado@hotmail.com

http://lattes.cnpq.br/7950707554487640

Leonardo Peres de Souza, Universidade Federal do Piauí, Parnaíba, Piauí, Brasil, souza_l_p@yahoo.com.br

http://lattes.cnpq.br/8998003020358863

Ricardo Toniolli, Universidade Estadual do Ceará, Faculdade de Veterinária, Departamento de Medicina Veterinária, Fortaleza, Ceará, Brasil, ricardo.toniolli@uece.br

http://lattes.cnpq.br/4931712701458453

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Published

2018-01-28

How to Cite

BARBOZA GUIMARÃES, D.; BANDEIRA BARROS, T.; FURTADO CANTANHÊDE, L.; NGUEKAM FEUGANG, J. M.; PERES DE SOUZA, L.; TONIOLLI, R. SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 19, p. 1–16, 2018. DOI: 10.1590/cab19138250. Disponível em: https://revistas.ufg.br/vet/article/view/e-38250. Acesso em: 17 jul. 2024.

Issue

Vol. 19 (2018): Continuous publication

Section

ZOOTECNIA

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