Qualidade espermática durante a curva de resfriamento do sêmen suíno diluído em água de coco em pó visando sua criopreservação

Daianny Barboza Guimarães; Tatyane Bandeira Barros; Ludymila Furtado Cantanhêde; Jean Magloire Nguekam Feugang; Leonardo Peres de Souza; Ricardo Toniolli

doi:10.1590/1809-6891v19e-38250

SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION

Authors

Daianny Barboza Guimarães Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil
Tatyane Bandeira Barros Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil
Ludymila Furtado Cantanhêde Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil
Jean Magloire Nguekam Feugang Mississipi State University, MSU, Jackson, MS, USA
Leonardo Peres de Souza Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil
Ricardo Toniolli Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil

DOI:

https://doi.org/10.1590/1809-6891v19e-38250

Keywords:

Sêmen suíno, Diluente alternative, Congelação, Viabilidade espermática

Abstract

Semen cryopreservation is associated with low productivity results. This study aimed to test the Coconut Water Powder (ACP®-103) as a ressuspension diluent after thawing semen, also evaluate sperm quality during the cooling curve until thawing of the semen. For this, the semen was collected from 15 boars once a week, incubated at 30 °C for 15 minutes, and afterwards, the samples were diluted in the diluent Beltsville Thawing Solution – BTS (Control) or ACP-103®, and subjected to a slow cooling curve, where the force and the motility were analyzed in each step. The thawed semen was ressuspended in its respective solvents and analyzed in the characteristics of vigor, motility, vitality, acrosome integrity and functionality of the membrane. During the analysis of vigor and motility that make up the cooling curve, and thawing, for analysis of vitality and intact acrosomal membrane, it was observed that there was no significant difference between treatments. Besides, after thawing, the BTS showed better results of sperm vigor, sperm motility and membrane functionality. However, the cooling curve and coconut water powder can be used in the protocol for cryopreservation of boar semen, as both ensured quality of sperm viability that may be used in artificial insemination.
Keywords: Swine semen; alternative extender; freezing; sperm viability.

Downloads

Download data is not yet available.

Downloads

Published

2018-01-28

How to Cite

GUIMARÃES, Daianny Barboza; BARROS, Tatyane Bandeira; CANTANHÊDE, Ludymila Furtado; FEUGANG, Jean Magloire Nguekam; SOUZA, Leonardo Peres de; TONIOLLI, Ricardo. SPERM QUALITY DURING THE COOLING CURVE OF SWINE SEMEN DILUTED IN COCONUT WATER EXTENDER AIMING YOUR CRYOPRESERVATION. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 19, p. 1–16, 2018. DOI: 10.1590/1809-6891v19e-38250. Disponível em: https://revistas.ufg.br/vet/article/view/e-38250. Acesso em: 5 dec. 2025.

Download Citation

Issue

Vol. 19 (2018): Continuous publication

Section

ANIMAL SCIENCE

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).