ISOLATION AND CULTURE OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE AND BONE MARROW OF DOGS

Authors

  • Endrigo Gabellini Leonel Alves Universidade de Uberaba (Uniube), Uberaba, Minas Geris, Brasil
  • Rogéria Serakides Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brasil
  • Isabel Rodrigues Rosado Universidade de Uberaba (Uniube), Uberaba, Minas Geris, Brasil
  • Jankerle Neves Boeloni Universidade Federal do Espírito Santo (UFES), Alegre, Espírito Santo, Brasil
  • Natalia Melo Ocarino Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brasil
  • Cleuza Maria de Faria Rezende Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brasil

DOI:

https://doi.org/10.1590/1089-6891v18e-34050

Keywords:

Animal Sanity

Abstract

The objective of this study was to establish a protocol for the isolation and culture of mesenchymal stem cells (MSC) from bone marrow and adipose tissue of dogs. Three 6-month-old male dogs were used. Approximately 3.0 cm3 of adipose tissue and 3.0 mL of bone marrow were collected. The samples were processed and the isolated cells were cultured in DMEM. The cells were subjected to phenotypic characterization and to osteogenic, adipogenic, and condrogenic differentiation to confirm the isolation of the MSC. The cells showed elongated and fusiform morphology and they were able to differentiate into osteoblasts, adipocytes, and chondrocytes. Phenotypic characterization revealed high expression of the MSC markers CD90(80.04%) and CD29(96%) in the cells from bone marrow and high expression of CD90(60.94%) and CD29(77.08%) in the cells from adipose tissue. In addition, phenotypic characterization revealed low expression of hematopoietic markers CD45(1.45%) and CD34(1.53%) in the cells from bone marrow and low expression of CD45(1.45%) and CD34(1.53%) in the cells from adipose tissue. Based on these results, the modifications applied to classical protocols simplified the process and proved to be efficient in the isolation, culture, and expansion of MSC isolated from the bone marrow and adipose tissue of dogs.
Keywords: cell culture; differentiation; regenerative medicine.

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Published

2017-07-31

How to Cite

ALVES, Endrigo Gabellini Leonel; SERAKIDES, Rogéria; ROSADO, Isabel Rodrigues; BOELONI, Jankerle Neves; OCARINO, Natalia Melo; REZENDE, Cleuza Maria de Faria. ISOLATION AND CULTURE OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE AND BONE MARROW OF DOGS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 18, 2017. DOI: 10.1590/1089-6891v18e-34050. Disponível em: https://revistas.ufg.br/vet/article/view/e-34050. Acesso em: 5 dec. 2025.

Issue

Vol. 18 (2017): Continuous publication

Section

VETERINARY MEDICINE

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