ELECTROPHORETIC PROFILE OF SERUM AND PERITONEAL PROTEINS OF HORSES SUBMITTED TO EXPERIMENTAL OBSTRUCTION OF DUODENUM, ILEUM AND LARGE COLON
DOI:
https://doi.org/10.5216/cab.v11i4.9699Keywords:
Animal SanityAbstract
Aiming at evaluating the electrophoresis profile of serum and peritoneal protein in horses submitted to an experimental model of intestinal obstruction, 24 animals were divided into four groups: instrumented control (GI), duodenum obstruction (GII), ileum obstruction (GIII) and large colon obstruction (GIV). Blood and peritoneal fluid samples were collected before the surgical procedures (T0), 3h after the obstruction (Ti) and 1, 3, 12, 24, 72, 120 and 168h after reperfusion (Tr). After sample centrifuging and fractioning, total protein were determined by biuret method, and the proteic fractions were separated by electrophoresis in agarose gel. Alterations were observed in serum and peritoneal concentrations of α, β and γ globulins on the studied animals. The changes occurred due to the intestinal inflammatory process caused by the obstruction model and the surgical trauma, associated to laparotomy. Animals submitted to duodenum and ileum obstruction showed higher serum and peritoneal concentrations of acute phase proteins. The electrophoretic separation of proteins included in the peritoneal liquid is more sensible when compared to the serum concentration to the diagnosis of abdominal inflammatory process.KEYWORDS: Acute phase protein, colic, horse.
Downloads
Download data is not yet available.
Published
2010-12-21
How to Cite
DI FILIPPO, P. A.; NOGUEIRA, A. F. da S.; ANAI, L. A.; ALVES, A. E.; SANTANA, A. E.; PEREIRA, G. T. ELECTROPHORETIC PROFILE OF SERUM AND PERITONEAL PROTEINS OF HORSES SUBMITTED TO EXPERIMENTAL OBSTRUCTION OF DUODENUM, ILEUM AND LARGE COLON. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 4, p. 938–946, 2010. DOI: 10.5216/cab.v11i4.9699. Disponível em: https://revistas.ufg.br/vet/article/view/9699. Acesso em: 22 nov. 2024.
Issue
Section
Veterinary Medicine
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
