In vitro antimicrobial activity of brazilian native fruit pomace extracts and essential oils against multidrug-resistant Pseudomonas aeruginosa of animal origin

Andryara Panizzon; Carla Patricia Freitas; Caroline Antunes do Nascimento; Lára Franco dos Santos; Luana Pasqualotto; Milena Zanoello Bertuol; Karen Apellanis Borges; Thales Quedi Furian; Letícia Trevisan Gressler; Luciana Ruschel dos Santos

doi:10.1590/1809-6891v26e-82765E

Authors

Andryara Panizzon Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brazil https://orcid.org/0009-0000-0123-2901
Carla Patricia Freitas Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brazil https://orcid.org/0000-0002-5329-7894
Caroline Antunes do Nascimento Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brazil https://orcid.org/0009-0009-4748-7331
Lára Franco dos Santos Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brazil https://orcid.org/0000-0003-3632-0172
Luana Pasqualotto Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brazil https://orcid.org/0009-0003-0822-0753
Milena Zanoello Bertuol Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brazil https://orcid.org/0000-0001-7096-0910
Karen Apellanis Borges Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil https://orcid.org/0000-0001-6649-5833
Thales Quedi Furian Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil https://orcid.org/0000-0003-0376-8616
Letícia Trevisan Gressler Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil https://orcid.org/0000-0001-9141-7503
Luciana Ruschel dos Santos Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brazil https://orcid.org/0000-0002-9771-7264

DOI:

https://doi.org/10.1590/1809-6891v26e-82765E

Abstract

Pseudomonas aeruginosa is a multidrug-resistant pathogen of growing concern in both human and veterinary medicine. Its increasing resistance profile, including that of animal-derived strains, necessitates alternative antimicrobial strategies within the One Health approach. This study evaluated the in vitro antimicrobial activities of four native Brazilian fruit pomace extracts (guabiroba, uvaia, araçá, and butiá) and four commercial essential oils (cinnamon, clove, ginger, and thyme) against clinical P. aeruginosa isolates obtained from domestic animals. Antimicrobial susceptibility and minimum inhibitory concentrations (MICs) were determined using the Kirby–Bauer disk diffusion method and a 96-well plate-based microdilution assay, respectively. The pomace extracts of guabiroba and uvaia showed inhibitory activity against 57.4 % of the tested isolates, whereas araçá and butiá exhibited no detectable effects. Cinnamon oil was the only compound that inhibited all isolates in both assays, with MIC values ranging from 4.88 to 9.70 µg/mL. The clove and thyme oils showed limited activity, whereas the ginger oil was largely ineffective. These findings highlight the potential of cinnamon oil as an antimicrobial candidate and suggest that native fruit residues may represent valuable, sustainable resources. Future studies should focus on investigating the mechanisms of action, synergistic effects, and in vivo efficacy of these compounds to support the development of phytotherapeutic alternatives against multidrug-resistant P. aeruginosa strains.

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