Manure production and mineral excretion in laying hens supplemented with lycopene and different mineral sources
Abstract
The effects of feeding diets containing lycopene and organic or inorganic minerals on commercial laying hens on manure production and characteristics were evaluated. A total of 192 laying hens were distributed into treatment groups using a completely randomized design with a 2 × 3 factorial arrangement (mineral sources × lycopene levels), with four replicates and eight birds per treatment. The diet was supplemented with lycopene at 0, 400, or 800 mg kg-1 of diet. The diets were as follows: MI, inorganic mineral diet without lycopene; MI+400, inorganic mineral diet with 400 mg of lycopene; MI+800, inorganic mineral diet with 800 mg of lycopene; MO, organic mineral diet without lycopene; MO+400, organic mineral diet with 400 mg of lycopene and MO+800, organic mineral diet with 800 mg of lycopene. The feed intake; daily manure production; and manure contents of dry matter, organic matter, mineral matter, ammoniacal nitrogen, carbon, and macro- and micronutrients; and manure pH were measured. Feed intake was higher in the MO and MO+800 groups than in the MI and MI+800 groups, with no differences in feed intake between the MI+400 and MO+400 groups. However, the feed intake was higher in the MI+400 group than in the MI and MI+800 group. The diet did not influence the daily amount of manure produced. The dry matter percentage in the manure of the MO+800 group was higher than that in all the other groups; the organic and mineral matter contents, including those of nitrogen, phosphorus, calcium, magnesium, iron, and zinc, were highest in the manure of the chickens in the MO+800 group that in the manure of the chickens from the other groups. The diets did not increase the daily amount of manure produced by the hens; however, the chickens in the MO+800 group excrete larger amounts of macro- and micronutrients.
Keywords: carotenoids; chelate; excretion; organic minerals; nutrients.
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1. Stefanello C, Santos TC, Murakami AE, Martins EN, Carneiro TC. Productive performance, eggshell quality, and eggshell ultrastructure of laying hens fed diets supplemented with organic trace minerals. Poultry Science. 2014; 93 :104–113. http://dx.doi.org/10.3382/ps.2013-03190
2. Carvalho KCN, Xavier CAN, Silva AA, Garcia ERM, Sanches DS, Kiefer C, Santos TMB. Qualitative and quantitative characterization of waste layers fed diets containing mineral sources and rosemary oil levels. Brazilian Animal Science. 2024; v.25, 77674E. doi: http://dx.doi.org/10.1590/1809-6891v25e-77674E
3. Qiu JL, Zhou Q, Zhu JM, Lu XT, Liu B, Yu DY, Lin G, Ao T, Xu JM. Organic trace minerals improve eggshell quality by improving the eggshell ultrastructure of laying hens during the late laying period. Poultry Science. 2020; 99:1483–1490. https://doi.org/10.1016/j.psj.2019.11.006
4. Kratzer FH, Vohra P. 1986 - Chelates in nutrition. 1st ed. Florida: CRC Press. 178 p. https://doi.org/10.1201/9781351070508
5. Stef DS, Gergen I. Effect of mineral-enriched diet and medicinal herbs on Fe, Mn, Zn, and Cu uptake in chicken. Chemistry Central Journal. 2012; v.6(19). http://journal.chemistrycentral.com/content/6/1/19
6. Garcia ERM, Chaves NRB, Oliveira CAL, Kiefer C, Melo EP. Performance and egg quality of laying hens fed with mineral sources and rosemary oil. Anais da Academia Brasileira de Ciências. 2019; v.91, e20180516. doi: https://doi.org/10.1590/0001-3765201820180516
7. Yunitasari F, Jayanegara A, Ulupi N. Performance, Egg Quality, and Immunity of Laying Hens due to Natural Carotenoid Supplementation: A Meta-Analysis. Food Sci. Anim. Resour. 2023; 43(2):282-304. https://doi.org/10.5851/kosfa.2022.e76
8. Toniazzo G, Ranna LF, Andrade TS, Pereira MFC, Henz EM, Gris B, Carvalho PLO, Rohloff Júnior N, Eyng C, Nunes RN. Mistura de óleos essenciais na dieta de frangos de corte sobre o desempenho, saúde intestinal e atividade de enzimas antioxidantes. Semina:Ciências Agrárias. 2025; v. 46, n. 4, p. 1005-1024. doi: https://doi.org/10.5433/1679-0359.2025v46n4p1005
9. Granja Planalto. Manual de manejo das poedeiras Dekalb Brown. 2009. Uberlândia: Côrtes.
10. Rostagno HS, Albino LFT, Donzele JL, Gomes PC, Oliveira RF, Lopes DC, Ferreira AS, Barreto SLT, Euclides RF. Tabelas brasileiras para aves e suínos: composição de alimentos e exigências nutricionais. 3ª ed. 2011.Viçosa, MG: UFV, DZO. 252 p.
11. Olson JB, Ward NE, Koutsos EA. Lycopene incorporation into egg yolk and effects on laying hen immune function. Poultry Science. 2008; 87:2573–2580. doi: https://doi.org/10.3382/ps.2008-00072
12. APHA. Standard Methods for the Examination of Water and Wastewater. 2017. (23rd ed.). Washington DC: American Public Health Association.
13. Bakhshalinejad R, Torrey S, Kiarie EG. Comparative efficacy of hydroxychloride and organic sources of zinc, copper, and manganese on egg production and concentration of trace minerals in eggs, plasma, and excreta in female broiler breeders from 42 to 63 weeks of age. Poultry Science. 2024; 103:103522. https://doi.org/10.1016/j.psj.2024.103522
14. Sahin K, Onderci M, Sahin N, Gursu MF, Khachikd F, Kucuke O. Effects of lycopene supplementation on antioxidant status, oxidative stress, performance and carcass characteristics in heat-stressed Japanese quail. Journal of Thermal Biology. 2006; 31, 307–312. doi: https://doi.org/10.1016/j.jtherbio.2005.12.006
15. Pozzo L, Tarantola M, Biasibetti E, Capucchio MT, Pagella M, Mellia E, Bergagna S, Gennero MS, Strazzullo G, Schiavone A. Adverse effects in broiler chickens fed a high lycopene concentration supplemented diet. Canadian journal of animal science. 2013; 93: 23124. doi: https://doi.org/10.4141/CJAS2012-081
16. Farias RM, Orrico Junior MAP, Orrico ACA, Garcia RG, Centurion SR, Fernandes ARM. 2012; Biodigestão anaeróbia de dejetos de poedeiras coletados após diferentes períodos de acúmulo. Ciência Rural, v.42, n.6, p.1089-1094.
17. Yenice E, Mizrak C, Gültekin M, Atik Z, Tunca M. Effects of Organic and Inorganic Forms of Manganese, Zinc, Copper, and Chromium on Bioavailability of These Minerals and Calcium in Late-Phase Laying Hens. Biol Trace Elem Res. 2015; 167(2):300-7. doi: https://doi.org/10.1007/s12011-015-0313-8
18. Orrico Junior MAP, Orrico ACA, Fava AF, Sunada NS, Schwingel AW, Garcia RG, Aspilcueta Borquis RR. 2018; Crude glycerin in co-composting with laying hen manure reduces N losses. Sci. Agric., v.75, n.5, p.361-367. http://dx.doi.org/10.1590/1678-992X-2016-0478
19. Schwingel AW, Orrico ACA, Lucas Junior J, Orrico Junior MAP, Aspilcueta Borquis RR, Fava AF. 2019; Laying hen manure in anaerobic Co-Digestion with glycerin containing different glycerol and impurity levels. Journal of Cleaner Production, 215, 1437e1444. https://doi.org/10.1016/j.jclepro.2019.01.125
20. Kunz A, Steinmetz RLR, Amaral AC. 2019; Fundamentos da digestão anaerobia, purificação do biogas, uso e tratamento do digestato. Concórdia: Sbera: Embrapa Suínos e Aves. 209p.
21. Feilberg A, Sommer S.G. 2013; Ammonia and malodorous gases: sources and abatement technologies. Animal Manure Recycling. Wiley: West Sussex, p.153-175. https://doi.org/10.1002/9781118676677.ch9
22. Carvalho LSS, Rosa DRV, Litz FH, Fagundes NS, Fernandes EA. 2015; Effect of the inclusion of organic copper, manganese, and zinc in the diet of layers on mineral excretion, egg production, and eggshell quality. Revista Brasileira de Ciência Avícola, p.87-92. doi: https://doi.org/10.1590/1516-635xSpecialIssue
23. Engin Y, Mizrak C, Gültekin M, Atik Z, Tunca M. 2015; Effects of organic and inorganic forms of manganese, zinc, copper, and chromium on bioavailability of these minerals and calcium in late-phase laying hens. Biological Trace Element Research, v.167, p.300-307. doi: https://doi.org/10.1007/s12011-015-0313-8
24. Sun Q, Guo Y, Li J, Zhang T, Wen J. 2012; Effects of methionine hydroxy analog chelated cu/mn/zn on laying performance, egg quality, enzyme activity and mineral retention of laying hens. The Journal of Poultry Science, v.49, p.20-25. doi: https://doi.org/10.2141/jpsa.011055
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