Ensaio HET-CAM para avaliação de colírio a base da casca e polpa de pequi (Caryocar brasiliense): estudo preliminar

João Marcelo Carvalho do Carmo; Bárbara Elisa Basilio de Oliveira; Nathany Arcaten; Mariana Ferreira da Silva; Cristiele Nunes Souto; Mônica  Rodrigues Ferreira Machado; Andréia Vitor Couto do Amaral

doi:10.1590/1809-6891v26e-79398E

Authors

João Marcelo Carvalho do Carmo Universidade Federal de Jataí (UFJ), Jataí, Goiás, Brazil https://orcid.org/0000-0002-6331-992X
Bárbara Elisa Basilio de Oliveira Universidade Federal de Jataí (UFJ), Jataí, Goiás, Brazil https://orcid.org/0009-0008-9024-6907
Nathany Arcaten Universidade Federal de Jataí (UFJ), Jataí, Goiás, Brazil https://orcid.org/0000-0001-9752-8095
Mariana Ferreira da Silva Universidade Federal de Jataí (UFJ), Jataí, Goiás, Brazil https://orcid.org/0000-0001-5114-431X
Cristiele Nunes Souto Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Rio de Janeiro, Brazil https://orcid.org/0000-0001-6714-3848
Mônica Rodrigues Ferreira Machado Universidade Federal de Jataí (UFJ), Jataí, Goiás, Brazil https://orcid.org/0000-0002-5715-4700
Andréia Vitor Couto do Amaral Universidade Federal de Jataí (UFJ), Jataí, Goiás, Brazil https://orcid.org/0000-0001-6406-2372

DOI:

https://doi.org/10.1590/1809-6891v26e-79398E

Abstract

Ophthalmic disorders of infectious or inflammatory origin can often affect companion animals. In this context, research related to Caryocar brasiliense has shown benefits such as antibiotic, antioxidant, antifungal, and anti-inflammatory action, and it is expected to be beneficial for ophthalmopathy treatment. This research aimed to develop solutions of pequi peel and pulp extracts and then perform preclinical tests to assess the irritating potential using the HET-CAM test through the appearance of vascular lesions, simulating the ocular conjunctiva. For this purpose, 56 embryonated chicken eggs were subjected to solutions of ethanol extract of peel (EEP) and hexane extract of pulp (HEP) at concentrations of 0.1, 0.2, 0.,25, 1.0, and 2.5% for eyedrops of peel (EPe) and 1.0, 1.5, 2.,0, 10.0, and 20.0% for eyedrops of pulp (EPu), in addition to negative controls, consisting of Tween at 0.5 and 1.0% and NaCl. The positive control was performed with 0.1 N NaOH, and bleeding, hyperemia, and coagulation reactions were observed. The second-order polynomial was the regression model that best fitted the data, presenting the ideal value for EPu of 0.18% and EPe of 0.24%. The therapeutic benefits of C. brasiliense and the low potential for irritation of the tested solutions indicated a promising use for ophthalmopathies.

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