Bovine tuberculosis diagnosis in the state of Bahia, Brazil, using the multiplex PCR technique
DOI:
https://doi.org/10.1590/1809-6891v25e-78409EAbstract
The rapid diagnosis of bovine tuberculosis (BT) allows official surveillance institutions to act
in areas that could be potential hotspots for the spread of the disease. The study aimed to identify
the Mycobacterium bovis agent by multiplex PCR of isolated colonies. Through the official inspection
service (Federal/State), samples suggestive of BT were collected during the post-mortem inspection
of bovine carcasses in slaughterhouses and sent to the laboratory for complementary diagnostics.
The samples were analyzed using bacteriological culture and Ziehl-Neelsen staining. In isolates
characterized as Acid-Alcohol Resistant Bacilli (BAAR), the multiplex PCR technique confirmed the agent
Mycobacterium bovis. Nine hundred ninety-one thousand three hundred sixteen cattle carcasses
were inspected, collecting 32 BT-suggestive samples in from animalsfrom 30 municipalities. Of these,
28.1% (9/32) showed growth on bacteriological culture media with BAAR. The BAAR isolates submitted
to multiplex PCR confirmed the presence of M. bovis. The multiplex PCR technique associated with
bacteriological examination and applied to post-mortem findings enabled the diagnosis of M. bovis in
the municipalities sampled in the Bahia state.
Downloads
References
Smaniotto, B, D.; Roca, R. O.; Barbosa, L. G. B.; Farias, I. M. S. C.; Brito, E. P.; Gallo, C. C.; PonteS, T. C. C.; Delbem, N. L. C. Bovine tuberculosis: impacts for livestock and risks for public ealth. Veterinaria e Zootecnia, 2019; v. 26: 45-59. Available from: link.gale.com/apps/doc/A634503934/AONE?u=uesc&sid=googleScholar&xid=045b95fd.
Murakami, P. S; Fuverki, R. B. N; Nakatani, S. M; Filho4, I. R. B; Biondo5, A. W. Tuberculose bovina: saúde animal e saúde pública. Arq. Ciênc. Vet. Zool. Unipar, Umuarama,2009; v. 12: 67-74 Available from: https://revistas.unipar.br/index.php/veterinaria/article/view/2936
» https://revistas.unipar.br/index.php/veterinaria/article/view/2936
Brasil. Ministério da Agricultura, Pecuária e Abastecimento. In: Manual Técnico do Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose Animal (PNCEBT). Brasília. p. 184, 2006. Portuguese. Available from: https://www.gov.br/agricultura/pt-br/assuntos/sanidade-animal-e-vegetal/saude-animal/programas-desaude-animal/pncebt
Oliveira, V.M.; Fonseca, A.H.; Pereira, M.J.S.; Carneiro, A.V.; Jesus, V.L.T.; Alves, P.A.M. Análise retrospectiva dos fatores associados à distribuição da tuberculose bovina no estado do Rio de Janeiro. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2008; v. 60: 574-579. Available from: https://doi.org/10.1590/S0102-09352008000300008
» https://doi.org/10.1590/S0102-09352008000300008
Costa, L. B. Caracterização da tuberculose bovina em regiões de relevância econômica no estado da Bahia. Universidade Federal da Bahia, Salvador. Bahia, 2012. Available from: (https://pesquisa.bvsalud.org/portal/resource/pt/vtt-402)
» https://pesquisa.bvsalud.org/portal/resource/pt/vtt-402
Mustafa, T. et al. Immunohistochemistry using a Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis. Modern Pathology, 2006; v. 19: 1606-1614, Available from: https://doi.org/10.1038/modpathol.3800697
» https://doi.org/10.1038/modpathol.3800697
Santos E.S.V; Alzamora F. F., Ribeiro B.S; Silva A.V.; Gomes H. M; Suffys P.N; and Costa J.N. Spoligotyping, genotyping, and spatial distribution of Mycobacterium bovis in cattle in the state of Bahia, Brazil. Pesquisa veterinária Brasileira. 2021 V.41: e06729. Available from: https://doi.org/10.1590/1678-5150-PVB-6729
» https://doi.org/10.1590/1678-5150-PVB-6729
Zanini, M. S; Moreira E.C; Lopes M.T.P; Oliveira R.S; Leão S.C; Fioravanti R.L; Roxo E; Zumarraga M; Romano M.I; Cataldi A; Salas C.E. Mycobacterium bovis: polymerase chain reaction identification in bovine lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism. Memórias do Instituto Oswaldo Cruz, 2001 v. 96: 809-813. Available from: https://doi.org/10.1590/S0074-02762001000600012
» https://doi.org/10.1590/S0074-02762001000600012
Pacheco, A. M.; Hamzè, A. L.; Avanza, M. F. B.; Pereira, D. M.; Pereira, R. E. P.; Cipriano, R. S.; Lot, R. F. S. Tuberculose bovina - relato de caso. Revista Científica Eletrônica de Medicina Veterinária, 2009; V:7 :13. Available from: https://faef.revista.inf.br/site/a/927-tuberculose-bovina-relato-de-caso.html
» https://faef.revista.inf.br/site/a/927-tuberculose-bovina-relato-de-caso.html
Alzamora F, F.; Vasconcellos, S. E.; Gomes, H. M.; Cavalcante, M. P.; Suffys, P. N.; Costa, J. N. Múltiplas estirpes de isolados de Mycobacterium bovis identificados por tipagem molecular em bovinos abatidos em matadourosfrigoríficos. Pesquisa Veterinária Brasileira, 2014; v: 34: 103-108. Available from: https://doi.org/10.1590/S0100736X2014000200001
» https://doi.org/10.1590/S0100736X2014000200001
Warren, R. M.; Pittius, N. C.; Barnard, M.; Hesseling, A. 1; Engelke, E.; De Kock, M.; Gutierrez, M. C. ; Chege, G. K. ; Victor, T. C.; Hoal, E. G.; Van Helden, P. D. Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference. The International Journal of Tuberculosis and Lung Disease,2006; v. 10: 818-822, 2006. Available from: (https://pubmed.ncbi.nlm.nih.gov/16850559/
» https://pubmed.ncbi.nlm.nih.gov/16850559/
Corner, L. A.; Melville L; Mccubbin K; Small KJ; Mccormick B.S; Wood P.R; Rothel J.S. Efficiency of inspection procedures for detection of tuberculosis lesions in cattle. Australian Veterinary Journal, 1990; v. 67: 389-392. Available from:( https://pubmed.ncbi.nlm.nih.gov/2085291/). Disponível em: https://doi.org/10.1111/j.1751-0813.1990.tb03020.x
» https://pubmed.ncbi.nlm.nih.gov/2085291/» https://doi.org/10.1111/j.1751-0813.1990.tb03020.x
Gathogo, S.M., Kuria, J.K.N.; Ombui, J.N. Prevalence of bovine tuberculosis in slaughter cattle in Kenya: a postmortem, microbiological and DNA molecular study. Tropical Animal Health Production, 2012. v. 44: 1739-1744. Available from: https://doi.org/10.1007/s11250-012-0131-3
» https://doi.org/10.1007/s11250-012-0131-3
Pereira J.D.B; Cerqueira V.D; Junior P.S.B; Bezerra D.K.O; Araújo F. R; Dias A.C.L; Araújo C.P; Correa G.R. Diagnóstico histopatológico e molecular de lesões sugestivas de tuberculose em búfalos abatidos nos municípios de Macapá e Santana, estado do Amapá. Pesquisa Veterinária Brasileira, 2017. v.37:1198-1204. Available from: https://doi.org/10.1590/S0100-736X2017001100003
» https://doi.org/10.1590/S0100-736X2017001100003
Cosivi O., Granja J., Daborn C., Raviglione M., Fujikura T., & Cousins D. Zoonotic tuberculosis due to Mycobacterium bovis in developing countries.Emerging Infectious Diseases, v. 4, p. 59-70, 1998, pmid: 9452399. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2627667/
» https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2627667/
Mota, P.M.P.C.; Lobato, F.C.F., Assis, R.A.; Lage, A.P., Parreiras, P.M. Isolamento de Mycobacterium bovis em cão. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2001; v.53:1-3. Available from: https://doi.org/10.1590/S0102-09352001000400003
» https://doi.org/10.1590/S0102-09352001000400003
Da Silva D.A.V. Comparação entre os métodos de diagnóstico da tuberculose em bovinos abatidos em matadouros - frigoríficos do estado de São Paulo. 2015. Dissertação - Faculdade de Ciências Agrárias e Veterinárias da UNESP. Disponível em: https://repositorio.unesp.br/items/133ccdee-db7d-491c-b4b6-769fc55ba968
» https://repositorio.unesp.br/items/133ccdee-db7d-491c-b4b6-769fc55ba968
Centro panamericano de zoonosis. Métodos de laboratório de micobateriologia veterinaria para el aislamento e identification de micobaterias. Ramos Meija, Buenos Aires, 1973. (Séries de Monografias científicas y Tecnicas, C.P.Z).
Abrahão, R. M. C. M. Tuberculose humana causada pelo Mycobacterium bovis: considerações gerais e a importância dos reservatórios animais. Archives of Veterinary Science, 1999. v. 4: 5-15. Available from: https://dx.doi.org/10.5380/avs.v4i1.3771
» https://dx.doi.org/10.5380/avs.v4i1.3771
Kazwala, R. R., Kambarag,E D. M., Daborn, C. J., Nyange, J., Jiwa, S. F. H., Sharp, J. M. Risk factors associated with the occurrence of bovine tuberculosis in cattle in the Southern Highlands of Tanzania, Veterinary Research Communications, Amsterdam,2001 v. 25: 609-614. Available from: https://doi.org/10.1023/a:1012757011524
» https://doi.org/10.1023/a:1012757011524
Campos, Dúnia Ibrahim et al. Condenação de carcaças bovinas por tuberculose, brucelose e cisticercose em abatedouro-frigorífico de Uberaba-MG e métodos de diagnóstico de tuberculose em carcaças. 2019. Dissertação - Universidade Federal de Uberlândia. Available from: https://doi.org/10.14393/ufu.te.2019.1263
» https://doi.org/10.14393/ufu.te.2019.1263
Pinsky, B. A.; Banaei, N. Multiplex Real-Time PCR Assay for Rapid Identification of Mycobacterium tuberculosis Complex Members to the Species Level,2008 v. 46: 2241-2246, 2008. Available from: https://doi.org/10.1128/JCM.00347-08
» https://doi.org/10.1128/JCM.00347-08
Ruggiero, A. P, A.A. Ikuno, V.C.A. Ferreira, E. Roxo. Tuberculose bovina: alternativas para o diagnóstico. Arq. Inst. Biol., São Paulo, v.74, n.1, p.55-65, jan./mar., 2007. Available from: https://doi.org/10.1590/18081657v74p0552007
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Brazilian Animal Science/ Ciência Animal Brasileira
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
