Bovine tuberculosis diagnosis in the state of Bahia, Brazil, using the multiplex PCR technique
Abstract
The rapid diagnosis of bovine tuberculosis (BT) allows official surveillance institutions to act
in areas that could be potential hotspots for the spread of the disease. The study aimed to identify
the Mycobacterium bovis agent by multiplex PCR of isolated colonies. Through the official inspection
service (Federal/State), samples suggestive of BT were collected during the post-mortem inspection
of bovine carcasses in slaughterhouses and sent to the laboratory for complementary diagnostics.
The samples were analyzed using bacteriological culture and Ziehl-Neelsen staining. In isolates
characterized as Acid-Alcohol Resistant Bacilli (BAAR), the multiplex PCR technique confirmed the agent
Mycobacterium bovis. Nine hundred ninety-one thousand three hundred sixteen cattle carcasses
were inspected, collecting 32 BT-suggestive samples in from animalsfrom 30 municipalities. Of these,
28.1% (9/32) showed growth on bacteriological culture media with BAAR. The BAAR isolates submitted
to multiplex PCR confirmed the presence of M. bovis. The multiplex PCR technique associated with
bacteriological examination and applied to post-mortem findings enabled the diagnosis of M. bovis in
the municipalities sampled in the Bahia state.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Brazilian Animal Science/ Ciência Animal Brasileira
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
