Reproductive efficiency of broiler breeder supplemented with canthaxanthin
DOI:
https://doi.org/10.1590/1809-6891v25e-77462EAbstract
The inclusion of substances with antioxidant properties in the diet of broiler breeders helps the enzymatic defense system in controlling the damage caused by free radicals in cells, for example, rooster spermatozoa. It is therefore hypothesized that supplementing broilers with canthaxanthin may have a positive effect on the reproductive system of broilers. The objective of this research was to study the action of the addition of canthaxanthin in the diet of broiler breeders on the fertilization and hatching rates of eggs and on the fertility of roosters. For the experiment, hens and roosters received, from 22 weeks of age, feed with and without supplementation of 6 ppm of canthaxanthin. Roosters between 30 and 50 weeks of age that received canthaxanthin in the diet showed less alterations in chromatin over the weeks of life. There were no differences between measurements of seminiferous tubules in different treatments. Canthaxanthin-supplemented broiler breeders had the highest rate of perforation of sperm in the perivitelline membrane, the best hatching rate and the highest fertility rate. It was concluded that the use of canthaxanthin as an antioxidant agent in the diet of broiler breeders improves reproductive efficiency.
Downloads
References
Opuwari CS, Henkel RR. An Update on Oxidative Damage to Spermatozoa and Oocytes. BioMed Research International, v.2016, p.1-11, 2016. Available from: https://doi.org/10.1155/2016/9540142.
Duarte V, Minafra CS, Santos FR, Perim FS. Inclusion of canthaxanthin and 25-hydroxycholecalciferol in the diet of broiler breeders on performance and incubation parameters. Ciência Rural. 2015; 45(11):2050-2055. Available from: https://doi.org/10.1590/0103-8478cr20140564 .
Bisht S, Faiq M, Tolahunase M, Dada R. Oxidative stress and male infertility. Nature Reviews Urology. 2017; 14(8):470-485. Available from: https://doi.org/10.1038/nrurol.2017.69
Bakst MR, Bauchan G. Apical blebs on sperm storage tubule epithelial cell microvilli: Their release and interaction with resident sperm in the turkey hen oviduct. Theriogenology. 2015; 83(9):1438–1444. Available from: https://doi.org/10.1016/j.theriogenology.2015.01.016.
Triques GE, Schmidt JM, Oro CS, Bordignon HF, Donin DG, Fernandes JIM. Effect of dietary antioxidant supplementation on reproductive characteristics of male broiler breeders during the post-peak production phase. Semina: Ciências Agrárias. 2016; 37(4):2557-2566. Available from: https://doi.org/10.5433/1679-0359.2016v37n4Supl1p2557
Rocha JSR, Barbosa VM, Lara LJC, Baião NC, Cançado SV, Lana AMQ, Pompeu MA, Vasconcelos RJC, Machado ALC, Miranda DJA, Fernandes MNS, Mendes PMM. Efeito do armazenamento e da cantaxantina dietética sobre a qualidade do ovo fértil e o desenvolvimento embrionário. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2013; 65(3):792-800. Available from: https://doi.org/10.1590/S0102-09352013000300027 .
Bramwell RK, Howarth BJr. Preferential attachment of cock spermatozoa to the perivitelline layer directly over the germinal disc of the hen's ovum. Biology Reproduction. 1992; 47(6):1113-1117. Available from: https://doi.org/10.1095/biolreprod47.6.1113 .
Donoghue AM. The effect of twenty-four hour in vitro storage on sperm hydrolysis through the perivitelline layer of ovipositioned turkey eggs. Poultry Science. 1996; 75(8):1035-1038. Available from: https://doi.org/10.3382/ps.0751035 .
Tolosa EMC, Rodrigues CJ, Behmer OA, Neto AGF. Manual de técnicas para histologia normal e patológica. 1st ed. São Paulo: Editora Manole, 2003. 341p. Portuguese.
Burrows WH, Quinn JP. The collection of spermatozoa from the domestic fowl and turkey. Poultry Science. 1937; 16(1):19-24. Available from: https://doi.org/10.3382/ps.0160019.
Pantos K, Sfakianoudis K, Maziotis E, Rapani A, Karantzali E, Gounari-Papaioannou A, Vaxevanoglou T, Koutsilieris M, Simopoulou M. Abnormal fertilization in ICSI and its association with abnormal semen parameters: a retrospective observational study on 1855 cases. Asian Journal of Andrology. 2021; 23(4):376-385. Available from: https://doi.org/10.4103/aja.aja_84_20.
Rumel D. “Odds ratio”: algumas considerações. Revista Saúde Pública. 1986; 20(3):253-258. Available from: https://doi.org/10.1590/S0034-89101986000300011.
Christensen VL, Fairchild BD, Ort DT,Nestor KE. Dam and sire effects on sperm penetration of the perivitelline layer and resulting fecundity of different lines of turkeys. Journal Applied of Poultry Research. 2005; 14(3):483-491. Available from: https://doi.org/10.1093/japr/14.3.483 .
Robertson L, Brown HL, Staines HJ, Wishart GJ. Characterization and application of an avian in vitro spermatozoa-egg interaction assay using the inner perivitelline layer from laid chicken eggs. Journal of Reproduction and Fertility. 1997; 110(2):205-211, 1997. Available from: https://doi.org/10.1530/jrf.0.1100205.
McGary S, Estevez I, Bakst MR, Pollock DL. Phenotypic traits as reliable indicators of fertility in male broiler breeders. Poultry Science. 2002; 81(1):102-111, 2002. Available from: https://doi.org/10.1093/ps/81.1.102.
Keel BA, Abney TO. Influence of bilateral cryptorchidism in the mature rat: Alterations in testicular function and serum hormone levels. Endocrinology. 1980; 107(4):1226-1233. Available from: https://doi.org/10.1210/endo-107-4-1226.
Heinlein CA, Chang C. Androgen receptor (AR) coregulators: an overview. Endocrine Reviews. 2002; 23(2):175-200. Available from: https://doi.org/10.1210/edrv.23.2.0460.
Yama OE, Duru FI, Oremosu AA, Noronha CC, Abayomi O. Stereological evaluation of the effects of Momordica charantia, antioxidants and testosterone on seminiferous tubules of rat. International Journal of Morphology. 2011; 29(3):1062-1068. Available from: http://dx.doi.org/10.4067/S0717-95022011000300068.
González-Morán MG, Guerra-Araiza C, Campos MG, Camacho-Arroyo I. Histological and sex steroid hormone receptor changes in testes of immature, mature and aged chickens. Domestic Animal Endocrinology. 2008; 35(4):371- 379. Available from: https://doi.org/10.1016/j.domaniend.2008.08.001.
Yoshida K, Muratani M, Araki H, Miura F, Suzuki T, Dohmae N, Katou Y, Shirahige K, Ito T, Ishii S. Mapping of hitone-binding site in histone replacement-completed spermatozoa. Nature Communications. 2018; 9(1):3885. Available from: https://doi.org/10.1038/s41467-018-06243-9.
Colaco S, Sakkas D. Paternal factors contributing to embryo quality. Journal of Assisted Reproduction and Genetics.2018;.35(11):1953-1968. Available from: https://doi.org/10.1007/s10815-018-1304-4 .
Beletti ME. Cromatina espermática: quebrando paradigmas. Revista Brasileira de Reprodução Animal. 2013; 37(2):92-96. Available from: http://cbra.org.br/pages/publicacoes/rbra/v37n2/pag92-96%20(RB465).pdf .
Johnson GD, Lalancette C, Linnemann AK, Leduc F, Boissonneault G, Krawetz SA. The sperm nucleus: chromatin, RNA, and the nuclear matrix. Reproduction. 2011; 141(1):21-36. Available from: https://doi.org/10.1530/REP-10-0322.
Carrel DT. Epigenetics of the male gamete. Fertility and Sterility. 2012; 97(2):267-274. Available from: https://doi.org/10.1016/j.fertnstert.2011.12.036.
Souza HM, Arruda LCP, Monteiro MM, Nery IHAV, Araújo Silva RAJ, Batista AM, Guerra MMP. The effect of canthaxanthin on the quality of frozen ram spermatozoa. Biopreservation and Biobanking. 2017; 15(3):220–227. Available from: https://doi.org/10.1089/bio.2016.0049.
Rodrigues ACN, Rocha JV, Beletti ME. Análise computacional da compactação da cromatina de espermatozoides de galo. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2009; 61(6):1302-1307. Available from: https://doi.org/10.1590/S0102-09352009000600008 .
Soares JM, Beletti ME. Avaliação da integridade cromatínica de espermatozóides de galos (Gallus gallus, Linnaeus, 1758) de linhagem pesada de duas idades. Brazilian Journal of Veterinary Research and Animal Science. 2006a; 42(4):543-553. Available from:
https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26471.
Rocha Júnior JM, Baião NC. Características físicas do sêmen de galos de matriz pesada com 35 e 68 semanas de idade. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2001; 53(6):683-685. Available from: https://doi.org/10.1590/S0102-09352001000600012.
Rosa AP, Scher A, Sorbara JO, Boemo LS, Forgiarini J, Londero A. Effects of canthaxanthin on the productive and productive and reproductive performance of broiler breeders. Poultry Science. 2012; 91(3):660-666. Available from: https://doi.org/10.3382/ps.2011-01582.
Soares JM, Beletti ME. Avaliação da morfologia e da compactação cromatínica em espermatozóides de galo (Gallus gallus, Linnaeus, 1758) através de microscopia electrônica de transmissão. Brazilian Journal of Veterinary Research and Animal Science. 2006b; 43(4):554-560. Available from: https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26472.
Beletti ME, Costa LF, Guardieiro MM. Morphometric features and chromatin condensation abnormalities evaluated by toluidine blue staining in bull spermatozoa. Brazilian Journal Morphology Science. 2005; 22(2):85-90. Available from: https://www.researchgate.net/publication/252360754 .
Ellington JE, Evenson DP, Fleming JE, Brisbois RS, Hiss GA, Broder SJ, Wright RW Jr. Coculture of human sperm with bovine oviduct epithelial cells decreases sperm chromatin structural changes seen during culture in media alone. Fertility and Sterility. 1998; 69(4):643- 649. Available from: https://doi.org/10.1016/S0015-0282(98)00023-5.
Twigg JP, Irvine DS, Aitken RJ. Oxidative damage to DNA in human spermatozoa does not preclude pronucleus formation at intracytoplasmic sperm injection. Human Reproduction. 1998; 13(7):1864-1871. Available from: https://doi.org/10.1093/humrep/13.7.1864 .
Bonagurio LP, Cruz FK, Kaneko IN, Matumoto-Pinto PT, Murakami AE, Santos TC. Dietary supplementation with canthaxanthin and 25-hydroxycholecalciferol has beneficial effects on bone and oxidative metabolism in European quail breeders. Poultry Science. 2020; 99(10):4874-4883. Available from: https://doi.org/10.1016/j.psj.2020.06.021 .
Bansal AK, Bilaspuri GS. Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International. 2010; 2011:686137. Available from: https://doi.org/10.4061/2011/686137.
Vanderhout SM, Rastegar Panah M, Garcia-Bailo B, Grace-Farfaglia P, Samsel K, Dockray J, Jarvi K, El-Sohemy A. Nutrition, genetic variation and male fertility. Translational Andrology and Urology. 2021; 10(3):1410-1431. Available from: https://doi.org/10.21037/tau-20-592.
Najafi L, Halvaei I, Movahedin M. Canthaxanthin protects human sperm parameters during cryopreservation. Andrologia. 2019; 51(10):e13389. Available from: https://doi.org/10.1111/and.13389.
Rosa AP, Bonilla CE, Londero A, Giacomini CB, Orso C, Fernandes MO, Moura JS, Hermes R. Effect of broiler breeders fed with corn or sorghum and canthaxanthin on lipid peroxidation, fatty acid profile of hatching eggs, and offspring performance. Poultry Science. 2017; 96(3):647–658. Available from: https://doi.org/10.3382/ps/pew294.
Araujo LF, Araujo CSS, Pereira RJG, Bittencourt LC, Silva CC, Cisneros F, Hermes RG, Sartore YGA, Dias MT. The dietary supplementation of canthaxanthin in combination with 25OHD3 results in reproductive, performance, and progeny quality gains in broiler breeders. Poultry Science. 2019; 98(11):5801-5808. Available from: https://doi.org/10.3382/ps/pez377.
Weir CP, Robaire B. Spermatozoa have decreased antioxidant enzymatic capacity and increased reactive oygen species production during aging in the brown Norway rat. Journal of Andrology. 2007; 28(2):229-240. Available from: https://doi.org/10.2164/jandrol.106.001362.
Rutz F, Anciuti MA, Pan EA Fisiologia e manejo reprodutivo de aves. In: Macari M, Mendes AA. 1st ed. Manejo de matrizes de corte, Campinas: FACTA, 2005. pp. 76-143.Portuguese.
Rutz F, Anciuti MA, Xavier EG, Roll VFB, Rossi P. Avanços na fisiologia e desempenho reprodutivo de aves domésticas. Revista Brasileira de Reprodução Animal. 2007; 31(3):307-317. Available from: http://cbra.org.br/pages/publicacoes/rbra/download/307.pdf.
Gumułka M, Kapkowska E. Age effect of broiler breeders on fertility and sperm penetration of the perivitelline layer of the ovum. Animal Reproduction Science. 2005; 90(1-2):135-148. Available from: https://doi.org/10.1016/j.anireprosci.2005.01.018.
Bakst, M.R. Role of the oviduct in maintaining sustaines fertility in hens. Journal Animal Science. 2011; 89(5):1323 -1329. Available from: https://doi.org/10.2527/jas.2010-3663.
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Brazilian Animal Science/ Ciência Animal Brasileira

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).























