Ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical training

Marcelle Bettio; Maiara Poersch Seibel; Maria Eduarda Mattos Franceschini; Rafaella Silva Rocha; Renata Lima Baptista; Anita Marchionatti Pigatto; João Antonio Tadeu Pigatto

doi:10.1590/1809-6891v24e-76196E

Authors

Marcelle Bettio Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0009-0008-3798-6682
Maiara Poersch Seibel Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-5295-4979
Maria Eduarda Mattos Franceschini Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-6705-2740
Rafaella Silva Rocha Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0001-8856-8239
Renata Lima Baptista Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-7138-9020
Anita Marchionatti Pigatto Universidade Estadual Paulista "Júlio de Mesquita Filho (UNESP), Botucatu, São Paulo, Brasil https://orcid.org/0000-0002-0521-7984
João Antonio Tadeu Pigatto Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-1541-9133

DOI:

https://doi.org/10.1590/1809-6891v24e-76196E

Abstract

The aim of this study was to evaluate and compare the effectiveness of three concentrations of gentian violet (0.5%, 0.1% and 0.05%) for staining the anterior capsule of the lens in horses. Thirty-six post-mortem equine eyes were collected. The eyes were subdivided into three groups composed of 12 eyes each, according to the concentration of gentian violet used. The effectiveness of staining the anterior capsule of the lens with different concentrations of gentian violet was assessed using an empirical system of evaluation on adequate or inadequate staining of capsular flaps. Based on the evaluation of the examiner, the 0.1% and 0.05% concentrations of gentian violet allowed adequate visualisation of the anterior capsule for continuous curvilinear capsulotomy training, whereas the 0.5% concentration produced strong and inadequate capsular staining. The model developed using gentian violet at concentrations of 0.1% and 0.05% allowed a clear visualisation of the capsular flap, which makes it viable as a model for training the continuous curvilinear capsulotomy step in cataract surgery in horses.
Keywords: anterior capsulotomy; vital dyes; equine; wet lab; surgical training

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