Avaliação dos colírios cetorolaco de trometamina e diclofenaco sódico sem conservantes no epitélio da córnea de coelhos

Fabiana Quartiero  Pereira; Melina Barbara Bender Macedo; Tanise  Carboni da Silva; Bruna  Santos dos Santos; Paula Stieven Hünning; Cláudia  Skilhan Faganello; Maira Haase Pacheco; João Roberto Braga de Mello; Fernanda Bastos de Mello; João Antonio Tadeu Pigatto

doi:10.1590/1809-6891v24e-75047E

Authors

Fabiana Quartiero Pereira Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0003-3854-0638
Melina Barbara Bender Macedo Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-0285-2296
Tanise Carboni da Silva Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0001-7523-4803
Bruna Santos dos Santos Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-5022-5422
Paula Stieven Hünning Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-0205-2598
Cláudia Skilhan Faganello Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-3224-3061
Maira Haase Pacheco Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0001-8298-6800
João Roberto Braga de Mello Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-7120-7709
Fernanda Bastos de Mello Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-2787-1768
João Antonio Tadeu Pigatto Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-1541-9133

DOI:

https://doi.org/10.1590/1809-6891v24e-75047E

Abstract

This study aimed to evaluate the corneal epitheliotoxic effects of preservative-free ketorolac tromethamine 0.5% and diclofenac sodium 0.1% eye drops in rabbits. Seventeen New Zealand rabbits were randomly divided into three groups: the 0.5% ketorolac tromethamine group, the 0.1% diclofenac sodium group, and the control group (0.9% NaCl). For each rabbit, both eyes were treated three times daily according to their treatment group. The corneal epithelia were analyzed using scanning electron microscopy to observe the number of light, grey, and dark cells; the number of epithelial holes; and the loss of hexagonal shape. Both of the formulations administered caused changes in the healthy corneal epithelia of rabbits. Except for number of epithelial holes (p < 0.05), all the parameters showed a statistically significant difference between the groups. The number of dark cells was highest in the ketorolac tromethamine group (p<0.05). The number of grey cells was higher in the diclofenac sodium group than in the control group (p =0.003). A higher number of dark cells was associated with a smaller number of light cells (r =-0.577, p < 0.001). Loss of shape showed a direct correlation with the number of dark cells (r=0.524, p=0.002). Based on the results presented, it was possible to conclude that ketorolac tromethamine 0.5% was more toxic to rabbit corneal epithelium than diclofenac sodium 0.1%.
Keywords: cornea; nonsteroidal anti-inflammatory agent; ophthalmic solutions; scanning electron microscopy

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