The Spectrophotometric method for determination of Colossoma macropomum (tambaqui) sperm concentration

Carminda Sandra Brito Salmito-Vanderley; Marcelo  José da Ascensão Feitosa Vieira; Thais Maia Torres; Marcos Luiz da Silva Apoliano; Carlos Henrique Sousa de Melo; Cristiane Clemente de Mello Salgueiro; José Ferreira Nunes

doi:10.1590/1809-6891v24e-74695E

Authors

Carminda Sandra Brito Salmito-Vanderley Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-2011-2021
Marcelo José da Ascensão Feitosa Vieira Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0003-2648-2936
Thais Maia Torres Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-7286-1788
Marcos Luiz da Silva Apoliano Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brasil https://orcid.org/0000-0002-4587-8509
Carlos Henrique Sousa de Melo Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil; Centro Universitário INTA (UNINTA), Sobral, Ceará, Brasil https://orcid.org/0000-0001-7848-6184
Cristiane Clemente de Mello Salgueiro Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0003-0071-4030
José Ferreira Nunes Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brasil https://orcid.org/0000-0003-1189-0937

DOI:

https://doi.org/10.1590/1809-6891v24e-74695E

Abstract

To assist the reproductive management of tambaqui (Colossoma macropomum) males in laboratory and commercial fish farming, a linear regression model was obtained from concentration curves using the spectrophotometric method. Twenty-two tambaqui males with an average age of three years old were selected and divided into two groups containing 11 animals each. Both groups alternately received a single dose of carp pituitary extract (CPE; 2.0 mg/kg body weight, intracoelomic). Sperm was collected 14 h after hormonal treatment and diluted (1:4000; sperm:formaldehyde saline). The concentration was estimated by counting spermatozoa in a Neubauer chamber and by using a spectrophotometer (λ=540 nm). Individual sperm concentration ranged from 11.40 to 71.13 × 109 sperm/mL. The degree of transmittance ranged from 62.1% to 95.0%. There was a significant correlation (r2 = 0.966; p < 0.0001) between sperm concentration analyzed in a Neubauer chamber and transmittance at 540 nm. Analysis by spectrophotometry and the prediction provided by the equation Y=100.293 – 0.509X proved to be an efficient and fast method for estimating sperm concentration in tambaqui and can be used in routine procedures in artificial fish reproduction laboratories.
Keywords: fish; reproduction; semen.

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References

Nynca J, Dietrich GJ, Liszewska E, Judycka S, Karol H, Dobosz S, Krom J, Ciereszko, A. Usefulness of a portable flow cytometer for sperm concentration and viability measurements of rainbow trout spermatozoa. Aquaculture. 2016; 451:353-356. (https://www.sciencedirect.com/science/article/abs/pii/S0044848615301873).

Yang H, Daly J, Tiersch TR. Determination of sperm concentration using flow cytometry with simultaneous analysis of sperm plasma membrane integrity in zebrafish Danio rerio. Cytometry Part A. 2016; 89(4):350-356. (https://pubmed.ncbi.nlm.nih.gov/26580311/).

Kowalski RA, Cejko BI. Sperm quality in fish: Determinants and affecting factors. Theriogenology. 2019; 135:94-108. (https://pubmed.ncbi.nlm.nih.gov/31203093/)

Magnotti C, Figueroa E, Farias JG, Merino O, Valdebenito I, Oliveira RPS, Cerqueira V. Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions. Animal Reproduction Science. 2018; 192:164-170. (https://pubmed.ncbi.nlm.nih.gov/29555193/)

Maria AN, Azevedo HC, Santos JP, Silva CA, Carneiro PCF. Semen characterization and sperm structure of the Amazon tambaqui Colossoma macropomum. Journal of Applied Ichthyology [Internet]. 2010 [cited 2022 Dez 01]; 26(5):779-783. Available from: https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1439-0426.2010.01542.x.

Kwantong S, Bart AN. Fertilization efficiency of cryopreserved sperm from striped catfish, Pangasius hypophthalmus (Sauvage), Aquaculture Research [Internet]. 2009 [cited 2022 Dez 01]; 40(3):292-297. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2109.2008.02088.x.

Leite LV, Melo MAP, Oliveira FCE, Pinheiro JPS, Campello CC, Nunes JF, Salmito-Vanderley CSB. Determinação da dose inseminante e embriogênese na fertilização artificial de tambaqui (Colossoma macropomum). Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2013; 65(2):421-429. (https://www.scielo.br/j/abmvz/a/Rg3Z4NmnC395s8cPbnk6BNr/?lang=pt).

Cejko BI, Sarosiek B, Krejszeff S, Kowalski RK. Multiple collections of common carp Cyprinus carpio L. semen during the reproductive period and its effects on sperm quality. Animal Reproduction Science. 2018; 188:178-188. (https://pubmed.ncbi.nlm.nih.gov/29223503/).

Egger RC, Motta NC, Franca TDS, Oliveira AV, Fernandes‐Braga W, Alvarez‐Leite JI, Murgas LDS. Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season. Aquaculture Research [Internet]. 2021 [cited 2022 Dez 01]; 52(12):6453-6463. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1111/are.15513#:~:text=In%20order%20to%20face%20seasonal,more%20prone%20to%20resist%20cryodamage.

Paulino MS, Miliorini AB, Murgas LDS, Felizardo VO. Desempenho reprodutivo do pacu, piracanjuba e curimba induzidos com extrato de buserelina. Boletim do Instituto de Pesca [Internet]. 2018 [cited 2022 Dez 01]; 37(1):39-45. Available from: https://www.pesca.agricultura.sp.gov.br/37_p1_39-45.pdf.

Cruz-Casallas PE, Medina-Robles VM, Velasco-Santamaría YM. Seasonal variation of sperm quality and the relationship between spermatocrit and sperm concentration in Yamu Brycon amazonicus. North American Jour of Aquaculture [Internet]. 2007 [cited 2022 Dez 01]; 69(2):159-165. Available from: https://afspubs.onlinelibrary.wiley.com/doi/abs/10.1577/a06-002.1.

Dong Q, Eudeline B, Huang C, Tiersch TR. Standardization of photometric measurement of sperm concentration from diploid and tetraploid Pacific oysters, Crassostrea gigas (Thunberg). Aquaculture Research [Internet]. 2005 [cited 2022 Dez 01]; 36(1):86-93. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2109.2004.01188.x.

Sanches EA, Marcos RM, Baggio DM, Tessaro L, Balen RE, Bombardelli RA. Estimativa da concentração espermática do sêmen de peixe pelo método de espermatócrito. Revista Brasileira de Zootecnia [Internet]. 2011 [cited 2022 Dez 03]; 40(6):1163-1167. Available from: https://www.scielo.br/j/rbz/a/yQR8SRQwsgbFxnPXGn5xsht/?lang=pt.

Shimoda E, Andrade DR, Vidal JMV, Yasui GS, Godinho HP, Silva JFS, Sousa G. Utilização do espermatócrito para estimar a concentração espermática no sêmen da piabanha (Brycon insignis). Brazilian Journal of Veterinary Research and Animal Science [Internet]. 2007 [cited 2022 Dez 04]; 44:19-24. Available from: https://www.revistas.usp.br/bjvras/article/view/26585.

Lu JC, Chen F, Xu HR, Wu YM, Xia XY, Huang YF, Lu NQ. Is flow cytometry really adapted to the determination of sperm concentration? Scandinavian Journal of Clinical and Laboratory Investigation. 2007; 67(4):394-401. (https://pubmed.ncbi.nlm.nih.gov/17558894/).

Petrunkina AM, Harrison RAP. Systematic misestimation of cell subpopulations by flow cytometry: a mathematical analysis. Theriogenology. 2010;73(7):839-847. (https://pubmed.ncbi.nlm.nih.gov/19896183/).

Cuevas-Uribe R, Tiersch TR. Estimation of fish sperm concentration by use of spectrophotometry. Cryopreservation in Aquatic Species. 2nd ed. Baton Rouge: World Aquaculture Society; 2011. p. 162-200.

Leclercq E, Antoni L, Bardon-Albaret A, Anderson CR, Somerset CR, Saillant EA. Spectrophotometric determination of sperm concentration and short-term cold-storage of sperm in Atlantic croaker Micropogonias undulatus L. broodstock. Aquaculture Research [Internet]. 2013 [cited 2022 Dez 04]; 45(8):1-12. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1111/are.12073.

Ciereszko A, Dabrowski K. Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using a spectrophotometric technique. Aquaculture. 1993; 109 (3-4):367-373. (https://www.sciencedirect.com/science/article/abs/pii/004484869390175X#:~:text=Sperm%20concentration%20significantly%20differed%20between,%C2%B12.57%C3%97109))

Hassan MM, Qin JG, Li X. Development of a spectrophotometric technique for sperm quantification in the spermcasting Australian flat oyster Ostrea angasi Sowerby. Aquaculture Research [Internet]. 2017 [cited 2022 Dez 02]; 48(9): 4844-4850. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1111/are.13304.

Hilsdorf AWS, Hallerman E, Valladão GMR, Zaminhan‐Hassemer M, Hashimoto DT, Dairiki JK, Takahashi LS, Albergaria FC, Gomes MES, Venturieri RLL, Moreira RG, Cyrino JEP. The farming and husbandry of Colossoma macropomum: From Amazonian waters to sustainable production. Reviews in Aquaculture [Internet]. 2021 [cited 2022 Nov 22]; 14(2): 993-1027. Available from: https://onlinelibrary.wiley.com/doi/10.1111/raq.12638.

FUNCEME - Fundação Cearense de Meteorologia e Recursos Hídricos Fortaleza: Relatório de Pluviometria por faixa de anos - Estado do Ceara, 1974-2008, anos 2007 e 2008, Município: Pentecoste, Posto: Pentecoste, Micro-região:11, Código: 115, Resumo de chuvas nos dados fornecidos pela FUNCEME [Internet]. 2009 [cited 2009 Jun 15]. Available from: http://www.funceme.br/rams/htm.

Fontenele O. Método de hipofisação de peixes adotado pelo DNOCS. 1st ed. Fortaleza: DNOCS; 1981. p. 33. Portuguese.

Silveira WF, Kavamoto ET, Rigolino MG, Tabata YAO. O método espectrofotométrico na avaliação da concentração de espermatozóides da truta arco-íris, Salmo irideus Gibbons. Boletim do Instituto da Pesca [Internet]. 1987 [cited 2022 Jun 20]; 14(1):69-73. Available from: https://www.pesca.agricultura.sp.gov.br/sumario14_unico.htm.

Viveiros ATM, Fessehaye Y, Ter-Veld M, Schulz RW, Komen J. Hand - stripping of semen and quality after maturational hormone treatments, in African catfish Clarias gariepinus. Aquaculture. 2002; 213:373-386. (https://www.sciencedirect.com/science/article/abs/pii/S0044848602000364?via%3Dihub).

Zaniboni-Filho E, Weingartner M. Técnicas de indução da reprodução de peixes migradores. Rev Bras Reprod Anim [Internet]. 2007 [cited 2022 Jun 17]; 31(3):367-373. Available from: http://www.cbra.org.br/pages/publicacoes/rbra/download/367.pdf.

Maria AN, Azevedo HC, Santos JP, Carneiro PCF. Hormonal induction and semen characteristics of tambaqui Colossoma macropomum. Zygote. 2012; 20(1):39-43. (https://pubmed.ncbi.nlm.nih.gov/21208496/).

Órfão LH, Nascimento AF, Corrêa FM, Cosson J, Viveiros ATM. Extender composition, osmolality and cryoprotectant effects on the motility of sperm in the Brazilian endangered species Brycon opalinus (Characiformes). Aquaculture. 2011; 311:241–247. (https://www.sciencedirect.com/science/article/pii/S0044848610008148?via%3Dihub).

Mylonas, CC; Duncan, NJ; Asturiano, JF. Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality. Aquaculture. 2017; 472:21-44. (https://www.sciencedirect.com/science/article/abs/pii/S004484861630206X?via%3Dihub).

Caldas JS, Silva ALS, Sousa LM, Sousa EB, Monteiro ILP, Barros FJT, Godoy, L. et al. Effects of hormonal treatment on induced spermiation and semen quality in the endangered Amazonian fish Hypancistrus zebra (Siluriformes, Loricariidae). Aquaculture. 2021; 533:736140. (https://www.sciencedirect.com/science/article/abs/pii/S0044848620338461?via%3Dihub).

Oliveira MS, Almeida-Monteiro PS, Nunes LT, Linhares FRA, Pinheiro JPS, Pinheiro RRR, Ferreira FO, Campelo CC, Salmito-Vanderley CSB. Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine. Semina: Ciências Agrárias [Internet]. 2016 [cited 2022 Ago 20]; 37(4):2167-2180. Available from: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21997/19459.

Martins EFF, Streit Jr DP, Abreu JS, Correia-Filho RAC, Oliveira CAL, Lopera-Barrero NM, Povh JA. Ovopel and carp pituitary extract for the reproductive induction of Colossoma macropomum males. Theriogenology. 2017; 98(1):57-61. (https://doi.org/10.1016/j.theriogenology.2017.04.047).

Oliveira FCE, Salmito-Vanderley CSB, Torres TM, Leite-Castro LV, Salgueiro CCM, Linhares FRA, Sales, Y. S, Nunes JF. Powdered coconut water with cryoprotectant can be used for cooling and future freezing of Tambaqui (Colossoma macropomum) sperm. Journal of Applied Ichthyology [Internet]. 2022 [cited 2022 Ago 20]; 38(3): 275-284. Available from: https://onlinelibrary.wiley.com/doi/10.1111/jai.14303.

Cabrita E, Martínez-Páramo S, Gavaia PJ, Riesco MF, Valcarce DG, Sarasquete C, Robles V. Factors enhancing fish sperm quality and emerging tools for sperm analysis. Aquaculture. 2014; 432: 389-401. (https://www.sciencedirect.com/science/article/abs/pii/S0044848614002105?via%3Dihub).

Nunes LT, Salmito-Vanderley CSB, Reis FYT, Neres RWP, Silva SQ. Reprodução de peixes reofílicos nativos do Brasil: fertilização artificial e qualidade da água. R. Bras. Reprod. Anim [Internet]. 2018 [cited 2022 Ago 20]; 42(1): 15-21. Available from: http://cbra.org.br/portal/downloads/publicacoes/rbra/v42/n1/p15-21%20(RB722).pdf.

Christensen P, Stryhn H, Hansen C. Discrepancies in the determination of sperm concentration using Bürker-Türk, Thoma and Makler counting chambers. Theriogenology. 2005; 63(4): 992-1003. (https://pubmed.ncbi.nlm.nih.gov/15710187/).

Björndahl MT, Christopher LR. Barratt. Raising Standards in Andrology Semen Analysis: Professional and Personal Responsibility. Journal of Andrology. 2004; 25(6): 862-863. (https://pubmed.ncbi.nlm.nih.gov/15477354/).

Loir M, Labbe C, Maisse G, Pinson A, Boulard G, Mourot B. Proteins of seminal fluid and spermatozoa in the trout (Oncorhynchus mykiss): partial characterization and variations. Fish Physiology and Biochemistry. 1990; 8(6): 485-495. DOI: (https://pubmed.ncbi.nlm.nih.gov/24221035/).

Scott A P, Baynes SM. A review of the biology, handling and storage of salmonid spermatozoa. Journal of Fish Biology [Internet]. 2006 [cited 2022 Ago 20]; 17(6):707-739. Available from: https://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1980.tb02804.x.