Extrato etanólico de açafrão (Curcuma longa L.) reduz apoptose e promove proliferação de células de osteossarcoma canino

Naira Moura Alves; Vanessa de Sousa Cruz; Leandro Lopes Nepomuceno; Nayane Peixoto Soares; Emmanuel Arnhold; Daniel Graziani; Patrícia de Almeida Machado Gonçalves; Guilherme Henrique Salazar Badan; Amanda da Matta Santos; Eugênio Gonçalves de Araújo

doi:10.1590/1809-6891v23e-72215E

Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation

Authors

Naira Moura Alves Universidade Federal de Goiás, Goiânia, Goiás, Brazil https://orcid.org/0000-0002-5377-3141
Vanessa de Sousa Cruz Centro Universitário Católica do Leste de Minas Gerais (UNILESTE), Coronel Fabriciano, Minas Gerais, Brasil https://orcid.org/0000-0002-8914-5964
Leandro Lopes Nepomuceno Centro Universitário Católica do Leste de Minas Gerais (UNILESTE), Coronel Fabriciano, Minas Gerais, Brasil https://orcid.org/0000-0001-5839-0046
Nayane Peixoto Soares Centro Universitário Cambury (Unicambury), Goiânia, Goiás, Brasil. https://orcid.org/0000-0001-9031-174X
Emmanuel Arnhold Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0003-0922-146X
Daniel Graziani Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-4997-2658
Patrícia de Almeida Machado Gonçalves Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-2681-5764
Guilherme Henrique Salazar Badan Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0001-5825-9398
Amanda da Matta Santos Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0003-3082-8856
Eugênio Gonçalves de Araújo Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-0242-2827

DOI:

https://doi.org/10.1590/1809-6891v23e-72215E

Abstract

Curcuma longa L., also known as turmeric, has been widely studied for its various therapeutic properties, including antineoplastic action. The ethanolic extract of the plant contains several phenolic compounds, especially curcumin. Osteosarcoma is a predominant bone tumor in dogs and humans, characterized by high metastatic potential and an unfavorable prognosis. The aim of this study was to investigate the effects of turmeric ethanol extract on canine osteosarcoma cells from established culture. The cells were cultured and treated with different curcumin concentrations (0, 10 μM, 20 μM, 50 μM, 100 μM, and 1000 μM) and exposure times (24h, 48h, and 72h). We first performed tetrazolium reduction technique (MTT) assay and calculated IC50. An immunocytochemistry assay was performed after extract treatment to verify the expression of mutated p53 and therefore study the proliferative potential of malignant cells; Bcl-2 and Ki-67 were used to assess apoptosis and the degree of malignancy, respectively. The extract enhanced the proliferation of canine osteosarcoma cells, reaching 3,819.74% at 50 μM of curcumin. The extract also significantly altered the expression of mutated p53 and Ki-67 proteins but not that of Bcl-2, suggesting that it did not induce this antiapoptotic pathway. Overall, these results are prerequisite to better understanding how natural compounds such as turmeric ethanolic extract affect cell proliferation and could be used to treat various diseases.
Keywords: bone neoplasms; D-17; natural compounds; therapeutic safety

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Published

2022-09-15

How to Cite

ALVES, N. M.; CRUZ, V. de S.; NEPOMUCENO, L. L.; SOARES, N. P.; ARNHOLD, E.; GRAZIANI, D.; GONÇALVES, P. de A. M.; BADAN, G. H. S.; SANTOS, A. da M.; ARAÚJO, E. G. de. Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 23, 2022. DOI: 10.1590/1809-6891v23e-72215E. Disponível em: https://revistas.ufg.br/vet/article/view/72715. Acesso em: 4 dec. 2024.

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Issue

Vol. 23 (2022): Continuous publication

Section

MEDICINA VETERINÁRIA

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