Influence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows

Francieli Berling; Fernanda Cavallari de Castro; Ana Carolina dos Santos Oliveira

doi:10.1590/1809-6891v23e-71852E

Authors

Francieli Berling Universidade Regional de Blumenau (FURB), Blumenau, Santa Catarina, Brasil https://orcid.org/0000-0003-1495-1774
Fernanda Cavallari de Castro Universidade Regional de Blumenau (FURB), Blumenau, Santa Catarina, Brasil https://orcid.org/0000-0002-3690-6379
Ana Carolina dos Santos Oliveira Universidade Regional de Blumenau (FURB), Blumenau, Santa Catarina, Brasil https://orcid.org/0000-0003-2456-1665

DOI:

https://doi.org/10.1590/1809-6891v23e-71852E

Abstract

The objective of this study was to evaluate the influence of thermal shock on oocytes used in the production of in vitro embryos (IVP) of high productivity Holstein cows on the day of follicular aspiration (OPU; 0), 30, 60 and 90 days before the OPU. From the mean temperature on day 0 and on the previous 30, 60 and 90 days, they were classified into comfort group (TC; up to 15°C) and heat stress (HS; above 15°C) groups. A negative influence was observed on oocytes and viable embryos (total and grade I). The heat stress in the periods of 30 and 60 days prior to OPU resulted in lower production of viable oocytes (P=0.0028; P=0.0092, respectively). Under stress, on the day of OPU (HS-OPU), cows showed no reduction in the amount of viable oocytes (P=0.5497) and there was no influence of temperature for the group stressed 90 days before OPU (P=0.8287). For total embryos, the difference occurred only in the HS-30 group (P=0.0317), where the groups HS-OPU, HS-60, HS-90 presented, respectively, P=0. 1987, P=0.0596 and P=0.4580. Regarding the production of embryos of grade 1, there was no difference for the groups HS-OPU (P=0.2291) and HS-90 (P=0.2868), but there was a reduction for HS-30 (P=0.0143) and HS-60 (P=0.0253). In summary, heat stress had a negative impact when it occurred 30 or 60 days before follicular aspiration. In addition, 30 days seems to be the period of more susceptibility and that causes the greatest deleterious effects on oocyte viability and IVP.
Keywords: Hyperthermia; Thermal shock; Ovum Pick Up; Dairy cows.

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Author Biography

Ana Carolina dos Santos Oliveira, Universidade Regional de Blumenau (FURB), Blumenau, Santa Catarina, Brasil

Docente da Universidade Regional de Blumenau

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