Activity of powdered methylxanthine applied to poultry litter on adults of Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae)

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DOI:

https://doi.org/10.1590/1809-6891v23e-71794E

Abstract

One of the pests that most affect and compromise poultry production worldwide is the insect Alphitobius diaperinus, known as the lesser mealworm. This insect is a vector of diseases that compromise not only chicken production but also human health. This study proposes to examine the efficacy and determine the appropriate rate of methylxanthine (MTX), a natural insecticide extracted from caffeine, for the control of an adult population of lesser mealworms in poultry litter. A total of 2,500 adult mealworms were distributed into five treatments in a completely randomized design using 10 replications with 50 insects per replication. The treatments consisted of a control group and four concentrations of MTX (14, 16, 18, and 20 g/m2) spread in plastic boxes containing reused poultry litter and feed, allocated in a broiler shed, to simulate the farm condition. The experimental period was 18 days, and five readings were performed on days 2, 4, 6, 10, and 18. Methylxanthine affected (P<0.05) the mealworms’ cumulative mortality rate, with the groups of insects housed in boxes treated with 16 g /m² MTX showing the highest cumulative mortality (86.6%) at the end of the experimental period. In conclusion, MTX has insecticidal action on adults of lesser mealworm and can be used on chicken litter to control the population of this insect in poultry sheds. The MTX concentration of 16 g/m2 showed the greatest effectiveness.
Keywords: chicken bed; insecticide; lesser mealworm; methylxanthine

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References

Volpato A, Galli GM, Campigotto G, Glombowsky P, Santos RCV, Silva AS, Vaucher RA. Avaliação in vitro dos efeitos inseticida e larvicida de oito óleos essenciais sobre o cascudinho aviário (Alphitobius diaperinus). Arch. Vet. Sci. 2018; 23(2):84-90. Disponível em: http://dx.doi.org/10.5380/avs.v23i2.46127

Axtell RC, Arends JJ. Ecology and management of arthropod pests of poultry. Annu. Rev. Entomol. 1990; 35(1990):101-126. Disponível em: https://doi.org/10.1146/annurev.en.35.010190.000533

Rueda LM, Axtell RC. Arthropods in litter of poultry (broiler chicken and turkey) houses. J. Agri. Entomol. 1997; 14(1):81-91. Disponível em: http://www.lib.ncsu.edu/resolver/1840.2/2110

Santos JC, Alves LFA, Opazo MAU, Mertz NR, Marcomini AM, Oliveira DGP, Bonini AK. Eficiência da aplicação de inseticida químico no solo para o controle de Alphitobius diaperinus Panzer (Coleoptera: Tenebrionidae) em aviário de frango de corte. Arq. Inst. Biol. 2009; 76(3):417-425. Disponível em: http://dx.doi.org/10.1590/1808-1657v76p4172009

Wojciehovski P, Pedrassani D, Fedalto LM. Terra de diatomáceas para controle do Alphitobius diaperinus em granjas de frango de corte. Saúde Meio Ambient. 2015; 4(1):66-78. Disponível em: https://doi.org/10.24302/sma.v4i1.695

Somerfield KG. Recent aspects of stored product entomology in New Zealand. New Zealand J. Agric. Res. 1981; 24(0):403-408. Disponível em: http://hbs.bishopmuseum.org/fiji/pdf/somerfield1981.pdf

Vaughan JA, Turner EC. Studies on the infestation into polystyrene insulation by the lesser mealworm (Alphitobius diaperinus Panz.), a common inhabitant of deep-pit caged layer houses. Va. J. Sci. 1982; 33(3):91-91.

Despins JL. Investigations of the destructive behavior, and methods for control of the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Ph.D. Tesisn, Blacksburg, Virgínia, 1987.

Despins JL, Turner EC, Ruszler PR. Effects of poultry manure moisture and poultry house construction materials on movements of the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), a structural insect pest in high-rise layer houses. Poult. Sci. 1989; 68(10):1326-1331. Disponível em: https://doi.org/10.3382/ps.0681326

Despins JL, Turner EC, Pfeifer DG. Evaluation of methods to protect poultry house insulation from infestation by lesser mealworm (Coleoptera: Tenebrionidae). J. Agric. Entomol. 1991; 8(3):209-217. Disponível em: http://hdl.handle.net/10919/73829

O’Connor JP. Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) damaging polystyrene insulation on an Irish piggery. Entomol. Monthly Magaz. 1987; 123(0):1472-1475.

Geden CJ, Axtell RC. Factors affecting climbing and tunneling behavior of the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae). J. Econom. Entomol. 1987; 80(6):1197-1204. Disponível em: https://doi.org/10.1093/jee/80.6.1197

Axtell RC. Biology and economic importance of the darkling beetle in poultry houses. Proceedings of the North Carolina State University Poultry Supervisors’ Short Course. 1994; 8-17. Disponível em: http://www.lib.ncsu.edu/resolver/1840.2/2108

Salin C, Delettre YR, Cannavaccioulo M, Vernon P. Spatial distribution of Alphitobius diaperinus (Panzer) (Coleopetera: Tenebrionidae) in the soil of a poultry house along a breeding cycle. Eur. J. Soil. Biol. 2000; 36(2):107-115. Disponível em: https://doi.org/10.1016/S1164-5563(00)01054-2

McAllister JC, Steelman CD, Skeeles JK. Reservoir competence of the lesser mealworm (Coleoptera: Tenebrionidae) for Salmonella typhimurium (Eubacteriales: Enterobacteriaceae). J. Med. Entomol. 1994; 31(3):369-372. Disponível em: https://doi.org/10.1093/jmedent/31.3.369

Crippen TL, Zheng L, Sheffield CL, Tomberlin JK, Beier RC, Yu Z. Transient gut retention and persistence of Salmonella through metamorphosis in the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae). J. Appl. Microbiol. 2012; 112(5):920-926. Disponível em: https://dx.doi.org/10.1111/j.1365-2672.2012.05265.x

Roche AJ, Cox NA, Richardson LJ, Buhr RJ, Cason JA, Fairchild BD, Hinkle NC. Transmission of Salmonella to broilers by contaminated larval and adult lesser mealworms, Alphitobius diaperinus (Coleoptera: Tenebrionidae). Poult. Sci. 2009; 88(1):44-48. Disponível em: https://doi.org/10.3382/ps.2008-00235

Strother KO, Steelman CD, Gbur EE. Reservoir competence of lesser mealworm (Coleoptera: Tenebrionidae) for Campylobacter jejuni (Campylobacterales: Campylobacteraceae). J. Med. Entomol. 2005; 42(1):42-47. Disponível em: https://doi.org/10.1093/jmedent/42.1.42

De Las Casas E, Pomeroy BS, Harein PK. Infection and quantitative recovery of Salmonella typhimurium and Escherichia coli from within the lesser mealworm, Alphitobius diaperinus (Panzer). Poult. Sci. 1968; 47(6):1871-1875. Disponível em: https://doi.org/10.3382/ps.0471871

De Las Casas E, Harein PK, Pomeroy BS. Bacteria and fungi within the lesser mealworm collected from poultry brooder houses. Environ. Entomol. 1972; 1(1):27-30. Disponível em: https://doi.org/10.1093/ee/1.1.27

McAllister JC, Steelman CD, Skeeles JK, Newberry LA, Gbur EE. Reservoir competence of the Alphitobius diaperinus (Coleoptera: Tenebrionidae) for Escherichia coli (Eubacteriales: Enterobacteriaceae). J. Med. Entomol. 1996; 33(6):983-987. Disponível em: https://doi.org/10.1093/jmedent/33.6.983

Eidson CS, Schmittle SC, Goode RB, Lal JB. The role of the darkling beetle (Alphitobius diaperinus) in the transmission of acute leukosis in chickens. Poult. Sci. 1965; 44(5):1366-1367. Disponível em: https://doi.org/10.3382/ps.0441347

De Las Casas E, Harein PK, Deshmukh DR, Pomeroy BS. Relationship between the lesser mealworm, fowl pox and Newcastle disease virus in poultry. J. Econ. Entomol. 1976; 69(6):775-779. Disponível em: https://doi.org/10.1093/jee/69.6.775

Despins JL, Axtell RC, Rives DV, Guy JS, Ficken MD. Transmission of enteric pathogens of turkeys by darkling beetle larva (Alphitobius diaperinus). J. Appl. Poult. Res. 1994; 3(1):61-65. Disponível em: https://doi.org/10.1093/japr/3.1.61

Watson DW, Guy JS, Stringham SM. Limited transmission of turkey coronavirus in young turkeys by adult Alphitobius diaperinus (Coleoptera: Tenebrionidae). J. Med. Entomol. 2000; 37(3):480-483. Disponível em: https://doi.org/10.1093/jmedent/37.3.480

Mullen G, Durden L. Medical and veterinary entomology. 3 rd ed. San Diego, United States, Academic Press, 2019;794p.

Elowni EE, Elbihari S. Natural and experimental infection on the beetle, Alphitobius diaperinus (Coleoptera: Tenebrionidae) with Choanotaenia infundibulum and other chicken tapeworms. Vet. Sci. Commun. 1979; 3:171-173. Disponível em: https://doi.org/10.1007/BF02268965

Galli A, Souza D, Garbellini GS, Coutinho CFB, Mazo LH, Avaca LA, Machado SAS. Utilização de técnicas eletroanalíticas na determinação de pesticidas em alimentos. Quím. Nova. 2006; 29(1):105-112. Disponível em: https://doi.org/10.1590/S0100-40422006000100020

Tomberlin JK, Richman D, Myers HM. Susceptibility of Alphitobius diaperinus (Coleoptera: Tenebrionidae) from broiler facilities in Texas to four insecticides. J. Econ. Entomol. 2008; 101(2):480-483. Disponível em: https://doi.org/10.1603/0022-0493(2008)101[480:soadct]2.0.co;2

Chernaki-Leffer AM, Sosagómez DR, Almeida LM, Lopes ION. Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrim, dichlorvos and triflumuron in southern Brazil. Rev. Bras. Entomol. 2011; 220(1):125-128. Disponível em: https://doi.org/10.1590/S0085-56262011000100020

Fogaça I, Ferreira E, Saturnino KC, Santos TR, Cavali J, Porto MO. Álcool para controle de cascudinho em cama de frangos de corte. Arch. Zootec. 2017; 66(256):509-514. Disponível em: https://doi.org/10.21071/az.v66i256.2766

Chernaki-Leffer AM, Sosa-Gomez DR, Almeida LM. Suscetibilidade de Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae) a reguladores de crescimento de insetos (RCI). Arq. Inst. Biol. 2006; 73(1):51-55. Disponível em: https://www.researchgate.net/publication/284549911_Susceptibility_of_Alphitobius_diaperinus_Panzer_1797_Coleoptera_Tenebrionidae_to_insect_growth_regulators_IGR

Gazoni FL, Flores F, Bampi RA, Silveira F, Boufleur R, Lovato. Avaliação da resistência do cascudinho (Alphitobius diaperinus) (Panzer) (Coleoptera: Tenebrionidae) a diferentes temperaturas. Arq. Inst. Biol. 2012; 79(1):69-74. Disponível em: https://www.scielo.br/j/aib/a/QDGt4PYpcqL76Yt3XtPH5bD/?format=pdf&lang=pt

Alves LFA, Uemura-Lima DH, Oliveira DGP, Godinho RPV. Eficiência de um novo inseticida comercial para o controle do cascudinho dos aviários (Alphitobius diaperinus) (Panzer) (Coleoptera: Tenebrionidae). Arq. Inst. Biol. 2010; 77(4):693-700. Disponível em: https://doi.org/10.1590/1808-1657v77p6932010

Dias DA, Vargas AB, Almeida FS. Efeitos de dosagem mais concentrada de cipermetrina no controle de cascudinho. Rev. Acad. Ciênc. Agrár. Ambient. 2013; 11(4):437-442. Disponível em: https://doi.org/10.7213/academico.011.004.AO11

Volpato A, Lorenzetti WR, Zortea T, Giombelli LCDD, Baretta D, Santos RCV, Vaucher RA, Raffin RP, Souza ME, Stefani LM, Boligon AA, Athayde ML, Silva AS. Melaleuca alternifolia essential oil against the lesser mealworm (Alphitobius diaperinus) and its possible effect on the soil fauna. Rev. Bras. Ciênc. Avíc. 2016; 18(1):41-46. Disponível em: https://doi.org/10.1590/1516-635X1801041-046

Maria CAB, Moreira RFA. Cafeína: revisão sobre métodos de análise. Quim. Nova. 2007; 30(1):99-105. Disponível em: http://static.sites.sbq.org.br/quimicanova.sbq.org.br/pdf/Vol30No1_99_20-RV05372.pdf

Krisko A, Kveder M, Pifat G. Effect of caffeine on oxidation susceptibility of human plasma low density lipoproteins. Clin. Chim. Acta. 2005; 355(1-2):47-53. Disponível em: https://doi.org/10.1016/j.cccn.2004.12.001

Varago FC, Silva LP, Ribeiro JR, Fernandes CA, Carvalho BC, Gioso MM, Moustacas VS. Teofilina como agente capacitante do semen bovino. Arq. Bras. Med. Vet. Zootec. 2017; 69(6):1670-1614. Disponível em: https://doi.org/10.1590/1678-4162-9173

Laranja AT, Manzato AJ, Bicudo HEMC. Effects of caffeine and used coffee grounds on biological features of Aedes aegypti (Diptera: Culicidae) and their possible use in alternative control. Genet. Mol. Biol. 2003; 26(4):419-429. Disponível em: https://doi.org/10.1590/S1415-47572003000400004

Guirado MM, Bicudo HEMMC. Attractiveness of bioinsecticides caffeine and used coffee grounds in the choice of oviposition site by Aedes aegypti (Diptera: Culicidae). Int. J. Mosquito Res. 2016; 47(3):47-51. Disponível em: https://docs.bvsalud.org/biblioref/ses-sp/2016/ses-38077/ses-38077-7041.pdf

Laranja AT, Manzato AJ, Bicudo HEMC. Caffeine effect on mortality and oviposition in successive generations of Aedes aegypti. Rev. Saúde Pública. 2006; 40(6):1112-1117. Disponível em: https://doi.org/10.1590/S0034-89102006000700022

Nathanson JA. Caffeine and related methylxanthines: possible naturally occurring pesticides. Science. 1984; 226(4671):184-187. Disponível em: https://doi.org/10.1126/science.6207592

Pólo AM. Efeito da cafeína no desenvolvimento de Aedes aegypti (Diptera: Culicidae): o significado biológico das alterações do padrão de síntese de esterases. Dissertação Mestrado. UNESP, 2014. Disponível em: http://www.sbicafe.ufv.br/handle/123456789/8993

Harel M, Kryger G, Rosenberry TL, Mallender WD, Lewis T, Fletcher RJ, Guss JM, Silman I, Sussman JL. Three-dimensional structures of Drosophila melanogaster acetylcholinesterase and of its complexes. Protein Sci. 2000; 9(6):1063-1072. Disponível em: https://dx.doi.org/10.1110%2Fps.9.6.1063

Davey K. The interaction of feeding and mating in the hormonal control of egg production in Rhodnius prolixus. J. Insect Physiol. 2007; 53(3): 208-215. Disponível em: https://doi.org/10.1016/j.jinsphys.2006.10.002

Lucena ALM, Gigliolli AAS, Lapenta AS. Análise das esterases durante as fases do desenvolvimento em Sitophilus oryzae (Coleoptera: Curculionidae) e sua relação com a resistência ao inseticida malathion. SaBios: Rev. Saúde Biol. 2012; 7(3):36-44. Disponível em: https://revista2.grupointegrado.br/revista/index.php/sabios/article/view/771

Pohanka M. Alpha-7-nicotinic acetylcholine receptor is a target in pharmacology and toxicology. Int. J. Mol. Sci. 2012; 13(2):2219-2238. Disponível em: https://dx.doi.org/10.3390%2Fijms13022219

Nishi Y, Sasaki K, Miyatake T. Biogenic amines, caffeine and tonic immobility in Tribolium castaneum. J. Insect Physiol. 2010; 56(6):622-628. Disponível em: https://doi.org/10.1016/j.jinsphys.2010.01.002

Mansur JF, Figueira-Mansur J, Santos AS, Santos-Junior H, Ramos IB, Medeiros MN, Machado EA, Kaiser CR, Muthukrishnan S, Masuda H, Melo ACA, Moreira MF. The effect of lufenuron, a chitin synthesis inhibitor, on oogenesis of Rhodnius prolixus. Pestic. Biochem. Physiol. 2010; 98(1):59-67. Disponível em: http://dx.doi.org/10.1016/j.pestbp.2010.04.013

Guirado MM, Bicudo HEMC. Effect of used coffee grounds on larval mortality of Aedes aegypti L. (Díptera: culicidae): Suspension concentration and age versus efficacy. BioAssay. 2007; 2(5):1-7. Disponível em: http://www.seb.org.br/biosay/arquivos/journals/1/articles/52/public/52-254-1-PB.pdf

Phankaen Y, Manaprasertsak A, Pluempanupat W, Koul O, Kainoh Y, Bullangpoti V. Toxicity and repellent action of Coffea arabica against Tribolium castaneum (Herbst) adults under laboratory conditions. J. Stored Prod. Res. 2017; 71(2017):112-118. Disponível em: https://doi.org/10.1016/j.jspr.2017.01.006

Fredholm BB, Bättig K, Holmén J, Nehlig A, Zvartau EZ. Actions of caffeine in the brain with special reference to factors that contribute to its widespread use. Pharmacol. Rev. 1999; 51(1):83-133. Disponível em: https://pharmrev.aspetjournals.org/content/51/1/83

Zahniser N, Simosky JK, Mayfield RD, Negri CA, Hanania T, Larson GA, Kelly MA, Grandy DK, Rubinstein M, Low MJ, Fredholm BB. Functional uncoupling of adenosine A(2A) receptors and reduced response to caffeine in mice lacking dopamine D2 receptors. J. Neurosci. 2000; 20(16):5949-5957. Disponível em: https://doi.org/10.1523/jneurosci.20-16-05949.2000

Crippen TL, Sheffield CL, Esquivel SV. The acquisition and internalization of Salmonella by the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae). Vector Borne Zoonotic Dis. 2009; 9(1):65-71. Disponível em: https://doi.org/10.1089/vbz.2008.0103

Lambkin TA, Rice SJ. Baseline responses of Alphitobius diaperinus (Coleoptera: Tenebrionidae) to cyfluthrin and detection of strong resistance in field populations in eastern Australia. J. Econ. Entomol. 2006; 99(3):908-913. Disponível em: https://doi.org/10.1603/0022-0493-99.3.908

Kaufman PE, Strong C, Rutz DA. Susceptibility of lesser mealworm (Coleoptera: Tenebrionidae) adults and larvae exposed to two commercial insecticides on unpainted plywood panels. Pest Manag. Sci. 2008; 64(2):108-111. Disponível em: https://doi.org/10.1002/ps.1475

Khoshraftar Z, Shamel A, Safekordi AA, Zaefizadeh M. Chemical composition of an insecticidal hydroalcoholic extract from tea leaves against green peach aphid. Int. J. Environ. Sci. Technol. 2019; 16(11):7583-7590. Disponível em: http://dx.doi.org/10.1007/s13762-018-2177-x

Gomes GA, Monteiro CMO, Julião LS, Maturano R, Senra TOS, Zeringóta V, Calmon F, Matos RS, Daemon E, Carvalho MG. Acaricidal activity of essential oil from Lippia sidoides on unengorged larvae and nymphs of Rhipicephalus sanguineus (Acari: Ixodidae) and Amblyomma cajennense (Acari: Ixodidae). Exp. Parasitol. 2014; 137(0):41-45. Disponível em: https://doi.org/10.1016/j.exppara.2013.12.003

Boursier CM, Bosco D, Coulibaly A, Negre M. Are traditional neem extract preparations as efficient as a commercial formulation of azadirachtin A?. Crop Prot. 2011; 30(3):318-322. Disponível em: https://doi.org/10.1016/j.cropro.2010.11.022

Anjali CH, Sharma Y, Mukherjee A, Chandrasekaran N. Neem oil (Azadirachta indica) nanoemulsion--a potent larvicidal agent against Culex quinquefasciatus. Pest Manag. Sci. 2012; 68(2):158-163. Disponível em: https://doi.org/10.1002/ps.2233

Yang Z, Baldermann S, Watanabe N. Recent studies of the volatile compounds in tea. Food Res. Int. 2013; 53(2):585-599. Disponível em: https://doi.org/10.1016/j.foodres.2013.02.011

Filomeno CA, Barbosa LCA, Teixeira RR, Pinheiro AL, Farias ES, Silva EMP, Picanço MC. Corymbia spp. and Eucalyptus spp. essential oils have insecticidal activity against Plutella xylostella. Ind. Crops Prod. 2017; 109(0):374-383. Disponível em: http://dx.doi.org/10.1016/j.indcrop.2017.08.033

Ananenka A. Insecticides and invertebrate neurophysiology: testing the efficacy of caffeine as an insecticide. 2018. Thesis (PhD in Biology). Montreal: Concordia University, 2018. Disponível em: https://digitalcommons.csp.edu/cup_commons_undergrad/124

Tamburro M, Sammarco ML, Trematerra P, Colacci M, Ripabelli G. Potential role of Alphitobius diaperinus Panzer (Insecta, Coleoptera) in poultry farm as transmission vector of bacterial pathogens in broilers and humans. J. Appl. Microb. 2022; 74(6):883-892. Disponível em: https://doi.org/10.1111/lam.13679

Rumbos C, Pantazis I, Athanassiou C. Population growth of the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae), on various commodities. J. Econ. Entomol. 2020; 113:1001-1007. Disponível em: https://doi.org/10.1093/jee/toz313

Oliveira DGP, Cardoso RR, Mamprim AP, Angeli LF. Laboratory and field evaluation of a cypermethrin-based insecticide for the control of Alphitobius diaperinus Panzer (Coleoptera: Tenebrionidae) and its in-vitro effects on Baeauveria bassiana bals. vuill. (Hypocreales: Cordycipitaceae). Braz. J. Poult. Sci. 2016; 18(3):371-380. Disponível em: https://dx.doi.org/10.1590/1806-9061-2015-0115

Souza CJ, Barbosa FM, Marujo MM, Santos ET, Domingues CH, Oliveira D, Sgavioli S. Effect of cypermethrin on the control of lesser mealworm (Alphitobius diaperinus) and broiler performance. Pesq. Vet. Bras. 2021; 41:e06859. Disponível em: https://doi.org/10.1590/1678-5150-PVB-6859

Tomberlin JK, Richman D, Myers H. Susceptibility of Alphitobius diaperinus (Coleoptera: Tenebrionidae) from broiler facilities in Texas to four insecticides. J. Econ. Entomol. 2008; 101(2):480-483. Disponível em: https://dx.doi.org/10.1603/0022-0493

Hickmann F, Morais AF, Bronzatto ES, Giacomelli T, Guedes JVC, Bernardi O. Susceptibility of the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae), from broiler farms of southern Brazil to insecticides. J. Econ. Entomol. 2018; 111(2):980-985. Disponível em: https://dx.doi.org/10.1093/jee/toy059

Gurib-Fakim A. Medicinal plants: traditions on yesterday and drugs of tomorrow. Mol. Aspects Med. 2006; 27(1):1-93. Disponível em: https://dx.doi.org/10.1016/j.mam.2005.07.008

López MD, Pascual-Villalobos MJ. Mode of inhibition of acetylcholinesterase by monoterpenoids and implications for pest control. Indust. Crops. Products. 2010; 31(2):284-288. Disponível em: https://dx.doi.org/10.1016/j.indcrop.2009.11.005

Published

2022-09-30

How to Cite

ISHIZUKA, M. M.; MOGYCA LEANDRO, N. S. M. L.; DA SILVA, J. M. S.; DOS SANTOS, R. R.; DE OLIVEIRA, H. F.; CAFÉ, M. B. Activity of powdered methylxanthine applied to poultry litter on adults of Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae). Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 23, 2022. DOI: 10.1590/1809-6891v23e-71794E. Disponível em: https://revistas.ufg.br/vet/article/view/71794. Acesso em: 22 dec. 2024.