Eritrograma e estresse oxidativo em bovinos confinados alimentados com feno de Brachiaria sp. e suplementados com antioxidantes

Roberta Dias da Silva Cunha; Gustavo Lage Costa; Ulisses Reis Correia Pinto; Juliana Job Serodio Ferezin; Paulo Henrique Jorge da Cunha; Maria Clorinda Soares Fioravanti

doi:10.1590/1809-6891v22e-70611E

Authors

Roberta Dias da Silva Cunha Universidade Federal de Goiás, Goiânia, Goiás, Brasil https://orcid.org/0000-0001-8301-4990
Gustavo Lage Costa Pontifícia Universidade Católica de Goiás (PUC-GO), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-3569-9566
Ulisses Reis Correia Pinto Instituto Federal Goiano - Campus Urutaí/Ceres, Urutaí, Goiás, Brasil https://orcid.org/0000-0001-5226-9659
Juliana Job Serodio Ferezin Universidade Federal de Goiás, Goiânia, Goiás, Brasil https://orcid.org/0000-0002-7734-0439
Paulo Henrique Jorge da Cunha Universidade Federal de Goiás, Goiânia, Goiás, Brasil https://orcid.org/0000-0002-1478-3670
Maria Clorinda Soares Fioravanti Universidade Federal de Goiás, Goiânia, Goiás, Brasil https://orcid.org/0000-0002-4993-5523

DOI:

https://doi.org/10.1590/1809-6891v22e-70611E

Abstract

Brachiaria sp contains sporidesmin that can be oxidized by lipoperoxidation and cause oxidative stress. In the present study we evaluated the effects of different antioxidants on lipoperoxidation of erythrocytes from Nelore cattle fed with Brachiaria sp hay. The experimental design was entirely randomized, in which 40 whole male cattle were divided into five treatments (G1: control - no supplementation; G2: selenium and vitamin E supplementation; G3: zinc supplementation; G4: selenium supplementation and G5: vitamin E supplementation) and allocated in feedlot pens for 105 days. The samples heparinized and with ethylenediaminetetraacetic acid (EDTA) were obtained every 28 days for hematological and oxidative stress evaluation (0, 28 56, 84 and 105 days). In the erythrogram total erythrocyte count, hemoglobin, and hematocrit (Ht) were measured. For the evaluation of oxidative stress, in order to analyze the characteristics of the erythrocyte membrane, the thiobarbituric acid reactive substances (TBARS), total glutathione (GSH-T), glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) were determined. The results showed that regardless of the treatment there was no oxidative stress during the experimental confinement period and that the joint association of selenium and vitamin E in the bovine diet provided a lower incidence of deleterious alterations on erythrocytes.
Key words: antioxidant enzymes; erythrocyte; reactive oxygen species (ROS); lipoperoxidation; Nelore.

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CEPEA 2020 - CEPAE/USP. Centro de Estudos Avançados em Economia Aplicada. Desenvolvido pela Universidade de São Paulo, 2020 [Internet]. Disponível em: https://cepea.esalq.usp.br/br/releases/cepea-retrospectivas-de-2020.aspx. Acesso em: 09 out 2021.

Seixas JN, Pinto CA, Rodrigues A, Tokarnia CH, França TN, Graça F.S, d´Avila MS, Peixoto PV. Comparative study between Brachiaria spp. and Pithomyces chartarum poisoning in cattle. Rev. Bras. Med. Vet. 2016; 38(Supl.2):1-10. https://rbmv.org/BJVM/article/view/192

Sordillo LM. Nutritional strategies to optimize dairy cattle immunity. J. Dairy Sci. 2016; 99(6):4967-4982. https://doi.org/10.3168/jds.2015-10354.

Brasil. Ministério da Agricultura, Pecuária e Abastecimento. Projeções do Agronegócio: Brasil 2012/2013 a 2022/2023. Assessoria de Gestão Estratégica, 4ª ed., Brasília, 2013. 96 p.

Soares CO, Rosinha GMS. Segurança alimentar, sustentabilldade e produção de proteína de origem animal. In. Vilela, E. F.; Callegaro, G. M.; Fernandes, G. W. Coord. Biomas e agricultura: oportunidades e desafios. Rio de Janeiro: Vertente edições, 2019. p.149-162.

Clayton MJ, Davis TZ, Knoppel EL, Stegelmeier BL. Hepatotoxic Plants that Poison Livestock. Vet. Clin. North Am. Food Anim. Pract. 2020; 36(3):715-723. https://doi.org/10.1016/j.cvfa.2020.08.003.

Motta AC, Riet-Correa Rivero G, Schild AL, Riet-Correa F, Méndez MC, Ferreira JL. Fotossensibilização hepatógena em bovinos no sul do Rio Grande do Sul. Ciênc. Rural. 2000; 30(1):143-149. https://doi.org/10.1590/S0103-84782000000100023.

Macedo MF, Bezerra MB, Soto Blanco B. Fotossensibilização em animais de produção na região semi-árida do Rio Grande do Norte. Arq. Inst. Biol. 2006; 73(2):251-254. https://www.researchgate.net/publication/242143971.

Vivanco RHC, Menge FGW, Barriga PAC. Variations of the erythrocyte osmotic fragility in cattle grazing on pastures with low selenium content with or without supplement with selenium. Rev. Cient. 2006; 16(3):227-231. https://www.researchgate.net/publication/286828714.

Cruz C, Driemeier D, Pires VS, Schenkel EP. Experimentally induced cholangiopathy by dosing sheep with fractionated extracts from Brachiaria decumbens. J. Vet. Diagn. Invest. 2001; 13(2):170-172. http://doi.org/10.1177/104063870101300215.

Brum KB, Haraguchi M, Garutti MB, Nóbrega FN, Rosa B, Fioravanti MCS. Steroidal saponin concentrations in Brachiaria decumbens and B. brizantha at diferent develomental stages. Ciênc. Rural. 2009; 39(1):279-281. https://doi.org/10.1590/S0103-84782008005000034.

Miles CO, Munday SC, Holland PT, Smith BL, Embling PP, Wilkins AL. Identification of a sapogenin glucoronide in the bile of sheep affected by Panicum dichotomiflorum toxicosis. N Z Vet J. 1991; 39(4):150-152. http://doi.org/10.1080/00480169.1991.35684.

Santos JCA, Riet-Correa F, Simiões SV, Barros CSL. Patogênese, sinais clínicos e patologia das doenças causadas por plantas hepatotóxicas em ruminantes e equinos no Brasil. Pesqui. Vet. Bras. 2008; 28(1):1-14. https://doi.org/10.1590/S0100-736X2008000100001.

Sies. H. 2019. Oxidative Stress: Eustress and Distress in Redox Homeostasis. In: Fink G. (Ed.) Stress: Physiology, Biochemistry, and Pathology, Handbook of Stress Series, London: Academic Press, Vol. 3, Chapter 13, p.153-163. https://doi.org/10.1016/B978-0-12-813146-6.00013-8.

Droge W. Free radicals in the physiological control of cell function. Physiol. Rev. 2002; 82(1):47-95. https://doi.org/10.1152/physrev.00018.2001.

Brezezinska-Slebodziska E. Species differences in the susceptibility of erythrocytes exposed to free radicals in vitro. Vet. Res. Comm. 2003; 27(3):211-217. https://doi.org/10.1023/a:1023344607691.

Oliveira S, Saldanha C. An overview about erythrocyte membrane. Clin. Hemorheol. Microcirc. 2010; 44(1):63-74. https://doi.org/10.3233/CH-2010-1253.

Pandey KB, Rizvi SI. Biomarkers of oxidative stress in red blood cells. Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub. 2011; 155(2):131-136. https://doi.org/10.5507/bp.2011.027.

Florin-Christensen J, Suarez CE, Florin-Christensen M, Wainszelbaum M, Brown WC, Mcelwain TF, Palmer GH. A unique phospholipid organization in bovine erythrocyte membranes. Proc. Natl. Acad. Sci. USA. 2001; 98(14):7736-7741. https://doi.org/ 10.1073/pnas.131580998.

Caldin M, Carli E, Furlanello T, Solano-Gallego L, Tasca S, Patron C, Lubas G. A retrospective study of 60 cases of eccentrocytosis in the dog. Vet. Clin. Pathol. 2005; 34(3):224-231. https://doi.org/ 10.1111/j.1939-165x.2005.tb00045.x.

Tang X, Xia Z, Yu J. An experimental study of hemolysis induced by onion (Allium cepa) poisoning in dogs. J. Vet. Pharmacol. Ther. 2008; 31(2):143-149. https://doi.org/ 10.1111/j.1365-2885.2007.00930.x.

Barbosa KBF, Costa NMB, Alfenas RCG, Paula SO, Minim VPR, Bressan J. Oxidative stress: concept, implications and modulating factors. Rev. Nutr. 2010; 23(4):629-643, https://doi.org/10.1590/S1415-52732010000400013

Cimen MY. Free radical metabolism in human erythrocytes. Clin. Chim. Acta. 2008; 390(1-2):1-11. https://doi.org/10.1016/j.cca.2007.12.025.

Todovora I, Simeonova G, Kyuchukova D, Dinev D, Gadjeva V. Reference values of oxidative stress parameters (MDA, SOD, CAT) in dogs and cats. Comp. Clin. Pathol. 2005; 13(4):190-194. https://doi.org/10.1007/s00580-005-0547-5.

Machado LP, Watanabe MJ, Kohayagawa A, Saito ME, Da Silveira VF, Yonezawa LA. Malondialdeído eritrocitário como índice de estresse oxidativo em equinos da raça Árabe. Rev. Bras. Hematol. Hemoter. 2007; 29:237. https://doi.org/10.1590/S0103-84782010005000094.

Ciampi F, Sordillo LM, Gandy JC, Caroprese M, Sevi A, Albenzio M, Santillo A. Evaluation of natural plant extracts as antioxidants in a bovine in vitro model of oxidative stress. J. Dairy Sci. 2020; 103(10):8938-8947. https://doi.org/10.3168/jds.2020-18182.

Spears JW, Weiss WP. Role of antioxidants and trace elements in health and immunity of transition dairy cows, Vet J. 2008; 176(1):70-76. https://doi.org/10.1016/j.tvjl.2007.12.015.

Mattioli GA, Rosa DE, Turic E, Picco SJ, Raggio SJ, Minervino AHH, Fazzio LE. Effects of Parenteral Supplementation with Minerals and Vitamins on Oxidative Stress and Humoral Immune Response of Weaning Calves. Animals (Basel). 2020; 10(8):1298. https://doi.org/10.3390/ani10081298.

NRC - NATIONAL RESEARCH COUNCIL. Nutrient requirements of beef cattle. 7.ed. Washington: National Academy Press, 1996. 242p.

Jain NC. Essentials of veterinary hematology. 4. ed. Philadelphia: Lea & Febiger, 1993. 407p.

Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 1990; 186:407-21. https://doi.org/10.1016/0076-6879(90)86134-h.

Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal. Biochem. 1969; 27(3):502-22. https://doi.org/10.1016/0003-2697(69)90064-5.

Bayoumi RA, Rosalki SB. Evaluation of methods of coenzyme activation of erythrocyte enzymes for detection of deficiency of vitamins B1, B2, and B6. Clin. Chem. 1976; 22(3):327-335.

Beutler E. Superoxide dismutase. In: Beutler E (Editor), Red Cell Metabolism. A Manual of Biochemical Methods. Philadelphia: Grune & Stratton, 1984. p.83-85.

Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105:121-126. https://doi.org/10.1016/s0076-6879(84)05016-3.

Fagliari JJ, Santana AE, Lucas FA, Campos Filho E, Curi PR. Constituintes sanguíneos de bovinos lactantes, desmamados e adultos das raças Nelore (Bos indicus) e Holandesa (Bos taurus), e de bubalinos (Bubalus bubalis) da raça Murrah. Arq. Bras. Med. Vet. Zootec. 1998; 50(3):263-271. https://repositorio.unesp.br/handle/11449/38249.

Machado LP, Kohayagawa A, Saito ME, Da Silveira VF, Yonezawa LA. Lesão oxidativa eritrocitária e mecanismos antioxidantes de interesse na Medicina Veterinária. Rev. Ciênc. Agrovet. 2009; 8(1):84-94. https://www.revistas.udesc.br/index.php/agroveterinaria/article/view/5317/3523

Sharma N, Singh NK, Singh OP, Pandey V, Verma PK. Oxidative stress and antioxidant status during transition period in dairy cows. Asian-Aust. J. Anim. Sci. 2011; 24(4):479-484. https://doi.org/ 10.5713/ajas.2011.10220.

Calamari L, Petrera F, Abeni F, Bertin G. Metabolic and hematological profiles in heat stressed lactating dairy cowsfed diets supplemented with different selenium sources and doses. Livest. Sci. 2011; 142(1-3):128-137. https://doi.org/10.1016/j.livsci.2011.07.005.

Kouryi JC, Donangelo CM. Zinco, estresse oxidativo e atividade física. Rev. Nutr. 2003; 16(4):433-441. https://doi.org/10.1590/S1415-52732003000400007.

Bian X, Teng T, Zhao H, Qin J, Qiao Z, Sun Y, Liun Z, Xu Z. Zinc prevents mitochondrial superoxide generation by inducing mitophagy in the setting of hypoxia/reoxygenation in cardiac cells. Free Radic. Res. 2018; 52(1):80-91. https://doi.org/10.1080/10715762.2017.1414949.

Kloubert V, Rink L. Zinc as a micronutrient and its preventive role of oxidative damage in cells. Food Funct. 2015;6(10):3195-3204. https://doi.org/ 10.1039/c5fo00630a.

Oteiza PI. Zinc and the modulation of redox homeosta- sis. Free Radic. Biol. Med. 2012; 53(9):1748-1759. https://doi.org/10.1016/j.freeradbiomed.2012.08.568.

Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990; 186:421-431. https://doi.org/10.1016/0076-6879(90)86135-i.

Liu ZL, Yang P, Chen P, Dong WX, Wang DM. Supplementation with selenium an vitamin E improves milk fat depression an fatty acid composition in dairy cows fed fat diet. Asian-Aust. J. Anim. Sci. 2008; 21(6): 38-844. https://doi.org/10.5713/ajas.2008.70618.

Križanović D, Sušić V, Božić P, Štoković I, Ekert-Kabalin A. Changes of bovine blood lipid peroxides and some antioxidants in the course of growth. Veterinarski Arhiv. 2008; 78 (4):269-278. https://hrcak.srce.hr/26534.

Chandra G, Aggarwal A, Singh K, Kumar M, Upadhyay RC. Effect of vitamin E and zinc supplementation on energy metabolites, lipid peroxidation, and milk production in peripartum sahiwal cows. Asian-Aust. J. Anim. Sci. 2013; 26(11):1569-1576. https://doi.org/10.5713/ajas.2012.12682.

Castillo C, Hernandez J, Bravo A, Lopez-Alonso M, Pereira V, Benedito JL. Oxidative status during late pregnancy and early lactation in dairy cows. Vet J. 2005; 169(2):286-292. https://doi.org/10.1016/j.tvjl.2004.02.001.

Castillo C, Hernandez J, Valverde I, Pereira V. Plasma malonaldehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows. Res. Vet. Sci. 2006; 80(2):133-139. https://doi.org/10.1016/j.rvsc.2005.06.003.

Kumar A, Gyanendra Singh BV, Meur SK. Modulation of antioxidant status and lipid peroxidation in erythrocyte by dietary supplementation during heat stress in buffaloes. Livest. Sci. 2011; 138(1-3):299-303. https://doi.org/10.1016/j.livsci.2010.12.021.

Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. Oxford: Clarendon Press, 2007, 543p.

Ma X, Deng D, Chen W. Inhibitors and Activators of SOD, GSH-Px, and CAT. In: Murat Senturk. Enzyme inhibitors and activators. 1nd ed. Croatia: IntechOpen; 2017. Chapter 9. https://doi.org/ 10.5772/65936.

Machefer G, Groussard C, Ranou-Bekono F, Zouhal H, Faure H, Vicent S, Cillard J, Gratas-Delamarche A. Extreme running competition decrease blood antioxidant defense capacity. J. Am. Coll. Nutr. 2004; 23(4):358-364. https://doi.org/10.1080/07315724.2004.10719379.

Abd Ellah MR, Okada K, Goryo M, Oishi A, Yasuda J. Superoxide dismutase activity as a measure of hepatic oxidative stress in cattle following ethionine administration. Vet. J. 2009; 182(2):336-341. https://doi.org/10.1016/j.tvjl.2008.05.013.

Dobbelaar P, Bouwstra RJ, Goselink RMA, Jorritsma R, Van Den Borne JJGC, Jansen EHJM. Effects of vitamin e supplementation on and the association of body condition score with changes in peroxidative biomarkers and antioxidants around calving in dairy heifers. J. Dairy Sci. 2010; 93(7):3103-3113. https://doi.org/ 10.3168/jds.2009-2677.

Felton GW, Summers CB. Antioxidant systems in insects. Arch. Insect Biochem. Physiol. 1995; 29(2):187-197. https://doi.org/10.1002/arch.940290208.

Linke A, Adams V, Schulze PC, Erbs S, Gielen S, Fiehn E. Antioxidative effects of exercise training patients with chronic heart failure increase in radical scavenger enzyme activity in skeletal muscle. Circulation. 2005; 111(14):1763-1770. https://doi.org/10.1161/01.CIR.0000165503.08661.E5.