LUTEAL BIOPSY IN NELLORE COWS

Authors

  • Ian Martin FMVZ/UNESP
  • Caroline Junko Fujihara FMVZ / Unesp
  • Wolff Camargo Marques Filho FMVZ / Unesp
  • Rosiara Rosárias Dias Maziero FMVZ / Unesp
  • Carmo Emanuel Almeida Biscarde FMVZ / Unesp
  • João Carlos Pinheiro Ferreira FMVZ /Unesp

DOI:

https://doi.org/10.5216/cab.v11i3.7012

Keywords:

Animal Sanity

Abstract

The present study describes a technique to obtain consecutive luteal samples by colpotomy. The animals received an epidural anesthesia and local anesthesia (vaginal vault) and after ten minutes the vaginal vault was incised with a scalpel blade and tissue was dissected to provide access to the pelvic cavity and to retract the ovary into the vagina. Then, a luteal biopsy was performed with a Yomann biopsy nipper. Signs indicative of pain and stress during the vaginal vault incision, traction of ovary or luteal biopsy were observed only in two collections. However, these signs were observed in ten collections during dissection of the vaginal wall and peritoneum. The occurrence of ataxia was observed in 26 collections and it was usually related to a longer duration of the procedure. Ataxia could be divided in light (15/26), moderate (6/26) and severe (5/26). The occurrence of ovarian adhesions ipsilateral to the incision was evaluated only in the initial four collections. Adhesions were present in 16 collections. The protocol described above provided a safe and efficient method to acquire luteal samples. The low incidence of adhesions allows the consecutive use of females without any interference in subsequent ovulations and collections.

KEYWORDS: Colpotomy, corpus luteum, luteal biopsy, Nellore (Bos indicus).

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Author Biographies

Ian Martin, FMVZ/UNESP

Aluno de Pós- Doutorado - Departamento de Reprodução Animal e Radiologia Veterinária - Faculdade de Medicina Veterinaria e Zootecnia - Unesp - Campus de Botucatu

Caroline Junko Fujihara, FMVZ / Unesp

Aluna de Doutorado - Departamento de Reprodução Animal e Radiologia Veterinária - Faculdade de Medicina Veterinaria e Zootecnia - Unesp - Campus de Botucatu

Wolff Camargo Marques Filho, FMVZ / Unesp

Aluno de Doutorado - Departamento de Reprodução Animal e Radiologia Veterinária - Faculdade de Medicina Veterinaria e Zootecnia - Unesp - Campus de Botucatu

Rosiara Rosárias Dias Maziero, FMVZ / Unesp

Aluna de Mestrado - Departamento de Reprodução Animal e Radiologia Veterinária - Faculdade de Medicina Veterinaria e Zootecnia - Unesp - Campus de Botucatu

Carmo Emanuel Almeida Biscarde, FMVZ / Unesp

Aluno de Mestrado - Departamento de Reprodução Animal e Radiologia Veterinária - Faculdade de Medicina Veterinaria e Zootecnia - Unesp - Campus de Botucatu

João Carlos Pinheiro Ferreira, FMVZ /Unesp

Prof. Adj. - Departamento de Reprodução Animal e Radiologia Veterinária - Faculdade de Medicina Veterinaria e Zootecnia - Unesp - Campus de Botucatu

Published

2010-10-02

How to Cite

MARTIN, I.; FUJIHARA, C. J.; MARQUES FILHO, W. C.; MAZIERO, R. R. D.; BISCARDE, C. E. A.; FERREIRA, J. C. P. LUTEAL BIOPSY IN NELLORE COWS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 3, p. 724–730, 2010. DOI: 10.5216/cab.v11i3.7012. Disponível em: https://revistas.ufg.br/vet/article/view/7012. Acesso em: 22 nov. 2024.

Issue

Section

Veterinary Medicine