Alterações laboratoriais inerentes à lesão renal aguda induzida por aminoglicosídeos em ratos wistar

Ana Carolina Queiroz Lima; Ana Bárbara Freitas  Rodrigues Godinho; Caio Vitor Bueno Dias; Vinícius Novaes Rocha; Hassan Jerdy

doi:10.1590/1809-6891v22e-70110

Authors

Ana Carolina Queiroz Lima Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos, Rio de Janeiro, Brasil https://orcid.org/0000-0002-4962-3394
Ana Bárbara Freitas Rodrigues Godinho Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos, Rio de Janeiro, Brasil https://orcid.org/0000-0002-4068-879X
Caio Vitor Bueno Dias Centro Universitário Luterano de Palmas, Palmas, Tocantins, Brasil https://orcid.org/0000-0002-9365-7145
Vinícius Novaes Rocha Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Minas Gerais, Brasil https://orcid.org/0000-0002-5656-0117
Hassan Jerdy Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos, Rio de Janeiro, Brasil https://orcid.org/0000-0002-2373-2453

DOI:

https://doi.org/10.1590/1809-6891v22e-70110

Abstract

Acute kidney injury (AKI) is defined as an increase greater than 0.3 mg/dL of serum creatinine within 48 hours and is a major cause
of death in patients in intensive care units. Twenty-four Wistar rats were divided into three groups: Control (0.9% saline), Genta
(gentamicin 50 mg.kg-1 BID) and Deh+Genta (gentamicin 50 mg.kg-1 BID + water restriction) and tested in an AKI model by
aminoglycoside administration and dehydration implementation. The animals in the Deh+Genta group exhibited the lowest average
weight and feed intake after the fifth day of the experiment. In this same period, water consumption by the Genta group was lower
than the Control group, but in the following days of the experiment, polydipsia was noted for this group. The Deh+Genta group
displayed the highest mean serum urea after the fifth day. The gentamicin-treated groups exhibited higher means than the Control
group for serum creatinine, which proved to be a late renal marker for AKI. Serum GGT was higher in the Deh+Genta group,
whereas urinary GGT was higher in the groups that received gentamicin, characterizing enzymuria, although severe dehydration
can mask the results by indicating false negative values. The urinary GGT enzyme did not act as an early AKI biomarker. Decreased
glomerular filtration rates enhanced the concentration of blood components and masked urinary and tissue components.
Keywords: Gentamycin; Rodents; Biochemical change.

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