HISTOLOGICAL, HISTOCHEMICAL AND ULTRASTRUCTURAL ANALYSIS OF THE DORSAL AND VENTRAL MUSCULATURE OF THE GUARU (Poecilia vivipara BLOCHI & SCHNEIDER, 1801)

Authors

  • Ana Paula Rezende dos Santos Universidade Federal de Goiás
  • Joana Cristina Neves de Menezes Faria Universidade Federal de Goiás
  • Simone Maria Teixeira de Sabóia-Morais Universidade Federal de Goiás

DOI:

https://doi.org/10.5216/cab.v11i4.6910

Keywords:

Animal Histology

Abstract

Poecilia vivipara (guaru), a neotropical, small, euryhaline fish, has been being used as a biomonitor. Dorsal and ventral muscles of Poecilia vivipara were analyzed to standardize methodologies for evaluating the normal tissue structure, in order to use them in toxicity studies. Thus, chemical and physical fixations were carried out, aiming at the histological and histochemical procedures of staining and reaction. The methods HE and MT revealed the organization of SSMF with epiaxial and hypaxial muscle bundles and associated conjunctive. MA marked the SSMF and facilitated the observation of sarcomeres in the presence of a thin metacromatically stained band. Part of the SSMF was positive PAS and reactive amylase, indicating the distribution of glycogen within the fibers. In the presence of SBB the fibers were reactive mainly in the dorsal region, indicating the presence of lipids. Cells with mitochondrial enzyme-rich cytoplasm were found in SDH pH 10.5. The SSMF stained with AT were arranged in alternating transverse bands, which correspond to the pattern seen under the MET. The use of these methodologies allowed the indication of Poecilia vivipara muscles as a tool for environmental variations studies, because its normal structure is standard in teleosts.

KEYWORDS: Morphology, muscle fiber, neotropical fish.

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Author Biographies

Ana Paula Rezende dos Santos, Universidade Federal de Goiás

Possui graduação em Ciências Biológicas, é mestranda em biologia celular e molecular, pela Universidade Federal de Goiás, e realiza pesquisas junto ao Laboratório de Comportamento Celular (ICB/UFG)

Joana Cristina Neves de Menezes Faria, Universidade Federal de Goiás

Graduada em Ciências Biológica licenciatura, pela Universidade Federal de Goiás, Mestre em Biologia Celular e Molecular pela mesma instituição e professora substituta de Biologia Celular e Histologia do Instituto de Ciências Biológicas da UFG

Simone Maria Teixeira de Sabóia-Morais, Universidade Federal de Goiás

possui graduação em Ciências Biológicas Licenciatura em Biologia pela Universidade Federal de Pernambuco (1986), graduação em Ciências Biológicas Bacharelado em Biologia pela Universidade Federal de Pernambuco (1987), mestrado em Ciências (Biologia Celular e Tecidual) pela Universidade de São Paulo (1992) e doutorado em Ciências (Biologia Celular e Tecidual) pela Universidade de São Paulo (1997). Faz parte do quadro de docentes do Programa de Pós-Graduação em Biologia - Área de Concentração: Biologia Celular. Desenvolve atividades de ensino e pesquisa em Popularização da Ciência pelo NETESB (Núcleo de Estudos em Tecnologias para Socialização do conhecimento em Biologia). Atualmente é professor Associado II da Universidade Federal de Goiás. Tem experiência na área de Morfologia, com ênfase em Ensino de graduação e Pós-graduação, Comportamento Celular, atuando principalmente nos seguintes temas: ecotoxicologia, biologia celular, biologia do desenvolvimento, comportamento celular, morfometria e histoquímica.

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Published

2010-12-21

How to Cite

SANTOS, A. P. R. dos; FARIA, J. C. N. de M.; SABÓIA-MORAIS, S. M. T. de. HISTOLOGICAL, HISTOCHEMICAL AND ULTRASTRUCTURAL ANALYSIS OF THE DORSAL AND VENTRAL MUSCULATURE OF THE GUARU (Poecilia vivipara BLOCHI & SCHNEIDER, 1801). Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 4, p. 906–913, 2010. DOI: 10.5216/cab.v11i4.6910. Disponível em: https://revistas.ufg.br/vet/article/view/6910. Acesso em: 24 nov. 2024.

Issue

Vol. 11 No. 4 (2010)

Section

Veterinary Medicine

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