Dietary supplementation of tilapia juveniles reared in bft (bioflocs) tanks with dl-methionine
DOI:
https://doi.org/10.1590/1809-6891v22e-63874Abstract
The present study aimed at determining the effects of dietary DL-methionine supplementation on the water quality, bioflocs composition and Nile tilapia juvenile's (initial body weight = 2.76 ± 0.06 g) growth performance in BFT rearing tanks (18 fish/100-L tank). Fish were or not subjected to artificial feed restriction. The experimental treatments consisted of two control groups: 1 – no feed restriction, no methionine supplementation; 2 – feed restriction at 25%, no methionine supplementation. There were also four treated groups: 1 - feed restriction at 25%, dietary DL-methionine supplementation at 0.5%; 2 - feed restriction at 25%, dietary DL-methionine supplementation at 1.0%; 3 – feed restriction at 25%, dietary DL-methionine supplementation at 2.0%; 4 – feed restriction at 25%, DL-methionine supplementation of molasses at 1.0. Supplementation of the commercial diet with DL-methionine has not affected either the water quality of the BFT Nile tilapia rearing tanks or the proximate composition of the bioflocs. After 8 weeks, weight gain of fish reared in tanks with feed restriction and dietary DL-methionine supplementation at 1% or 2% has not differed (P>0.05) from the tanks without feed restriction. In conclusion, it is possible to restrict the daily feed allowances of Nile tilapia juveniles reared in BFT tanks at 25%, with no growth performance impairment, if a minimal dietary DL-methionine supplementation of 1.0% is given.
Keywords: Bioflocs. Amino acid. Aquaculture. Water quality.
Downloads
References
De Schryver P, Crab R, Defoirdt T, Boon N, Verstraete W. The basics of bio-flocs technology: the added value for aquaculture. Aquaculture. 2008 Jun; 277(3-4): 125-137. Disponível em: http://www.sciencedirect.com/science/article/pii/S0044848608000896.
Kumar V S, Pandey P K, Anand T, Bhuvaneswari G R, Dhinakaran A, Kumar S. Biofloc improves water, effluent quality and growth parameters of Penaeus vannamei in an intensive culture system. Journal of Environmental Management. 2018 Jun; 215(1): 206-215. Disponível em: http://www.sciencedirect.com/science/article/pii/S030147971830238X.
Azim M E, Little D C. The biofloc technology (BFT) in indoor tanks: Water quality, biofloc composition, and growth and welfare of Nile tilapia (Oreochromis niloticus). Aquaculture. 2008 Oct; 283(1-4):29–35. Disponível em: http://www.sciencedirect.com/science/article/pii/S0044848608004699.
Avnimelech Y. Biofloc technology: A pratical guide book. Baton Rouge, Louisiana, United States, The World Aquaculture Society. 2009, 182 p.
Long L, Yang J, Li Y, Guan C, Wu F. Effect of biofloc technology on growth, digestive enzyme activity, hematology, and immune response of genetically improved farmed tilapia (Oreochromis niloticus). Aquaculture. 2015 Nov; 448 (1): 135-141. Disponível em: http://www.sciencedirect.com/science/article/pii/S0044848615300089
Kuhn D D, Boardman G D, Lawrence A L, Marsh L, Flick G J. Microbial flocs generated in bioreactors is a superior replacement ingredient for fishmeal or soybean meal in shrimp feed. Aquaculture. 2009 Nov; 296(1-2): 51–57. Disponível em: http://www.sciencedirect.com/science/article/pii/S0044848609006619.
Xu W J, Pan L Q, Zhao D H, Huang J. Preliminary investigation into the contribution of bioflocs on protein nutrition of Litopenaeus vannamei fed with different dietary protein levels in zero-water exchange culture tanks. Aquaculture. 2012 Jun; 350(1): 147-153. Disponível em: http://www.sciencedirect.com/science/article/pii/S0044848612002074.
da Silva M A, de Alvarenga E R, Alves G F O, Manduca L G, Turra E M, de Brito T S, de Sales S C M, da Silva Junior A F, Borges W J M, Teixeira E A. Crude protein levels in diets for two growth stages of Nile tilapia (Oreochromis niloticus) in a biofloc system. Aquaculture research. 2018 Jun; 49(8): 2693-2703. Disponível em: https://doi.org/10.1111/are.13730.
Green B W, Rawles S D, Schrader K K, Gaylord T G, McEntire M E. Effects of dietary protein content on hybrid tilapia (Oreochromis aureus × O. niloticus) performance, common microbial off-flavor compounds, and water quality dynamics in an outdoor biofloc technology production system. Aquaculture. 2019 Mar; 503(1): 571-582. Disponível em: http://www.sciencedirect.com/science/article/pii/S0044848618320805.
Emerenciano M, Ballester E L, Cavalli R O, Wasielesky W. Effect of biofloc technology (BFT) on the early postlarval stage of pink shrimp Farfantepenaeus paulensis: growth performance, floc composition and salinity stress tolerance. Aquaculture International. 2011 Oct; 19(5): 891-901. Disponível em: https://doi.org/10.1007/s10499-010-9408-6.
Wei Y F, Liao S A, Wang A L. The effect of diferente carbon sources on the nutritional composition, microbial community and structure of bioflocs. Aquaculture. 2016 Dec; 465(1): 88-93. Disponível em: http://www.sciencedirect.com/science/article/pii/S0044848616304410.
Ballester E L C, Abreu P C, Cavalli R O, Emerenciano M, De Abreu L, Wasielesky Jr W. Effect of practical diets with different protein levels on the performance of Farfantepenaeus paulensis juveniles nursed in a zero exchange suspended microbial flocs intensive system. Aquaculture Nutrition. 2010 Mar; 16(2): 163-172. Disponível em: https://doi.org/10.1111/j.1365-2095.2009.00648.x.
Durigon E G, Lazzari R, Uczay J, de Alcântara Lopes D L, Jerônimo G T, Sgnaulin T, Emerenciano M G C. Biofloc technology (BFT): Adjusting the levels of digestible protein and digestible energy in diets of Nile tilapia juveniles raised in brackish water. Aquaculture and Fisheries. 2020 Ján; 5(1): 42-51. Disponível em: https://doi.org/10.1016/j.aaf.2019.07.001.
Bauer W, Prentice-Hernandez C, Tesser M B, Wasielesky Jr W, Poersch L H. Substitution of fishmeal with microbial floc meal and soy protein concentrate in diets for the pacific white shrimp Litopenaeus vannamei. Aquaculture. 2012 Apr; 342-343: 112-116. Disponível em: http://www.sciencedirect.com/science/article/pii/S0044848612001147.
Ekasari J, Angela D, Waluyo S H, Bachtiar T, Surawidjaja E H, Bossier P, De Schryver P. The size of biofloc determines the nutritional composition and the nitrogen recovery by aquaculture animals. Aquaculture. 2014 Apr; 426-427: 105-111. Disponível em: https://doi.org/10.1016/j.aquaculture.2014.01.023.
Li J, Liu G, Li C, Deng Y, Tadda M A, Lan L, Zhu S, Liu D. Effects of different solid carbon sources on water quality, biofloc quality and gut microbiota of Nile tilapia (Oreochromis niloticus) larvae. Aquaculture. 2018 Oct; 495:919-931. Disponível em: https://doi.org/10.1016/j.aquaculture.2018.06.078.
Avnimelech Y. Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture. 1999 Jun; 176(3-4): 227-235. Disponível em: https://doi.org/10.1016/S0044-8486(99)00085-X.
Caldini N N, Capistrano H H D A, Rocha Filho P R N, Carmo e Sá M V. Partial replacement of artificial diets by wet bioflocs biomass in Nile tilapia culture tanks. Acta Scientiarum. Animal Sciences. 2018 Oct; 40:1-8. Disponível em: https://doi.org/10.4025/actascianimsci.v40i1.42426
Clesceri L S, Greenberg A E, Eaton A D. Standard Methods for the Examination of Water and Wastewater. 20.ed. Washington, DC: American Public Health Association; 1998.
Boyd C E, Tucker C S. Water quality and pond soil analyses for aquaculture. Auburn University, Opelika, USA, 1992. 183pp.
AOAC. Association of Official Analytical Chemistry. Official Methods of Analysis, 17th ed, Washington, D.C.USA, 2000.
Harris, T.D., Graham, J.L. Predicting cyanobacterial abundance, microcystin, and geosmin in a eutrophic drinking-water reservoir using a 14-year dataset. Lake and Reservoir Management. 2017. 33. Disponível em: https://pubs.er.usgs.gov/publication/70181018
da Costa D A, de Souza C L, Saliba E D O S, Carneiro J D. By-products of sugar cane industry in ruminant nutrition. Int. J. Adv. Agric. Res. 2015 Feb; 3: 1-95. Disponível em: http://www.bluepenjournals.org/ijaar/pdf/2015/March/da_Costa_et_al.pdf.
Xu W, Morris T C, Samocha T M. Effects of C/N ratio on biofloc development, water quality, and performance of Litopenaeus vannamei juveniles in a biofloc-based, high-density, zero-exchange, outdoor tank system. Aquaculture. 2016 Feb; 453:169-175. Disponível em: ttps://doi.org/10.1016/j.aquaculture.2015.11.021.
Zhang J, Chen L, Dong H, Duan Y, Li Z, Wen G, Chen J, Zhenhua Feng Z, Xu W, Xie J. Artificial substrates in zero‐water‐exchange culture system regulate the rearing performance of Pacific white shrimp Litopenaeus vannamei (Boone, 1931) under the winter indoor condition. Aquaculture research. 2016 Ján; 47(1): 91-100. Disponível em: ttps://doi.org/10.1111/are.12473.
Hargreaves, J.A. Biofloc Production Systems for Aquaculture. Southern Regional Aquaculture Centre, No. 4503, 2013.
Ebeling J M, Timmons M B, Bisogni J J. Engineering analysis of the stoichiometry of photoautotrophic, autotrophic, and heterotrophic removal of ammonia-nitrogen in aquaculture systems. Aquaculture. 2006 Jun; 257(1-4): 346-358. Disponível em: https://doi.org/10.1016/j.aquaculture.2006.03.019.
Luo G, Zhang N, Cai S, Tan H, Liu Z. Nitrogen dynamics, bacterial community composition and biofloc quality in biofloc-based systems cultured Oreochromis niloticus with poly-β-hydroxybutyric and polycaprolactone as external carbohydrates. Aquaculture. 2017 Oct; 479: 732-741. Disponível em: https://doi.org/10.1016/j.aquaculture.2017.07.017.
Luo G, Gao Q, Wang C, Liu W, Sun D, Li L, Tan H. Growth, digestive activity, welfare, and partial cost-effectiveness of genetically improved farmed tilapia (Oreochromis niloticus) cultured in a recirculating aquaculture system and an indoor biofloc system. Aquaculture. 2014 Feb; 422-423: 1-7. Disponível em: https://doi.org/10.1016/j.aquaculture.2013.11.023.
Mansour A T, Esteban M A. Effects of carbon sources and plant protein levels in a biofloc system on growth performance, and the immune and antioxidant status of Nile tilapia (Oreochromis niloticus). Fish & shellfish immunology. 2017 May; 64: 202-209. Disponível em: https://doi.org/10.1016/j.fsi.2017.03.025.
Ray A J, Lewis B L, Browdy C L, Leffler J W. Suspended solids removal to improve shrimp (Litopenaeus vannamei) production and an evaluation of a plant-based feed in minimal-exchange, superintensive culture systems. Aquaculture. 2010 Feb; 299(1-4): 89–98. Disponível em: https://doi.org/10.1016/j.aquaculture.2009.11.021.
Gallardo-Collí A, Pérez-Rostro C I, Hernández-Vergara M P. Reuse of water from biofloc technology for intensive culture of Nile tilapia (Oreochromis niloticus): effects on productive performance, organosomatic indices and body composition. International Aquatic Research. 2019 Feb; 11(1): 43-55. Disponível em: https://link.springer.com/article/10.1007/s40071-019-0218-9.
Rajkumar M, Pandey P K, Aravind R, Vennila A, Bharti V, Purushothaman C S. Effect of different biofloc system on water quality, biofloc composition and growth performance in Litopenaeus vannamei (Boone, 1931). Aquaculture research. 2016 May; 47(11): 3432-3444. Disponível em: https://doi.org/10.1111/are.12792.
Valle B C S, Dantas Jr E M, Silva J F X, Bezerra R S, Correia E S, Peixoto S R M, Soares R B. Replacement of fishmeal by fish protein hydrolysate and biofloc in the diets of Litopenaeus vannamei postlarvae. Aquaculture Nutrition. 2015 Nov; 21(1): 105-112. Disponível em: https://doi.org/10.1111/anu.12149.
He J Y, Long W Q, Han B, Tian L X, Yang H J, Zeng S L, Liu Y J. Effect of dietary l‐methionine concentrations on growth performance, serum immune and antioxidative responses of juvenile Nile tilapia, Oreochromis niloticus. Aquaculture research. 2017 Oct; 48(2): 665-674. Disponível em: https://doi.org/10.1111/are.12913.
Yuan Y C, Gong S Y, Yang H J, Lin Y C, Yu D H, Luo Z. Effects of supplementation of crystalline or coated lysine and/or methionine on growth performance and feed utilization of the Chinese sucker, Myxocyprinus asiaticus. Aquaculture. 2011 Jun; 316(1-4): 31-36. Disponível em: https://doi.org/10.1016/j.aquaculture.2011.03.015
Guo, T Y, Zhao W, He J Y, Liao S Y, Xie J J, Xie S W, Masagounder K, Liu Y J, Tian L X, Niu J. Dietary dl-methionyl-dl-methionine supplementation increased growth performance, antioxidant ability, the content of essential amino acids and improved the diversity of intestinal microbiota in Nile tilapia (Oreochromis niloticus). British Journal of Nutrition. 2020 Aug; 123(1): 72-83. Disponível em: https://doi.org/10.1017/S0007114519002289.
Cavalcante D D H, Lima F R D S, Rebouças V T, Carmo e Sá M V. Nile tilapia culture under feeding restriction in bioflocs and bioflocs plus periphyton tanks. Acta Scientiarum. Animal Sciences. 2017 Jul/Set; 39(3): 223-228. Disponível em: https://doi.org/10.4025/actascianimsci.v39i3.33574
Figueiredo‐Silva, C., Lemme, A., Sangsue, D., & Kiriratnikom, S. Effect of DL‐methionine supplementation on the success of almost total replacement of fish meal with soybean meal in diets for hybrid tilapia (Oreochromis niloticus × Oreochromis mossambicus). Aquaculture Nutrition. 2015 Aug; 21(2): 234-241. Disponível em: https://doi.org/10.1111/anu.12150.
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 CIÊNCIA ANIMAL BRASILEIRA (Brazilian Animal Science)

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).