Estabelecimento de um protocolo de soroaglutinação rápida (SAR) para detecção de anticorpos para Salmonella Typhimurium em suínos

Rosecler Alves Pereira; Marisa Macagnan; Patricia Schwarz; Claudio Wageck Canal; Verônica Schmidt

doi:10.5216/cab.v11i3.6100

RAPID AGGLUTINATION TEST (RAT) FOR THE DETECTION OF ANTI-Salmonella Typhimurium ANTIBODY IN SWINE

Authors

Rosecler Alves Pereira Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil
Marisa Macagnan Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil
Patricia Schwarz Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil
Claudio Wageck Canal Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil
Verônica Schmidt Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil

DOI:

https://doi.org/10.5216/cab.v11i3.6100

Keywords:

Animal Sanity

Abstract

Salmonella Typhimurium is an important agent isolated from cases of human food poisoning in Brazil. Animal-origin products are the main source of infection, and pork has been implicated in Salmonella transmission to humans. Programs to salmonella monitoring in swine have been carried out, using bacteriological and serological tests like ELISA. However, these tests are time-consuming and expensive. The rapid agglutination test (RAT) is cheaper, faster and easier. Aiming to standardize RAT to detect anti-salmonella antibodies in swine serum, 60 samples of swine serum were tested. They had previously displayed positive (30) or negative (30) results in the ELISA test. The results showed that RAT had sensitivity, specificity, predictive positive value and predictive negative value equal to 96.7% when non-diluted serum was tested. Thus, this test can be applied to detect antibodies against S. Typhimurium in swine serum.

KEYWORDS: Antibody, diagnosis, pig, rapid agglutination test, Salmonella Typhimurium.

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Published

2010-10-02

How to Cite

PEREIRA, R. A.; MACAGNAN, M.; SCHWARZ, P.; CANAL, C. W.; SCHMIDT, V. RAPID AGGLUTINATION TEST (RAT) FOR THE DETECTION OF ANTI-Salmonella Typhimurium ANTIBODY IN SWINE. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 3, p. 677–682, 2010. DOI: 10.5216/cab.v11i3.6100. Disponível em: https://revistas.ufg.br/vet/article/view/6100. Acesso em: 22 apr. 2025.

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Issue

Vol. 11 No. 3 (2010)

Section

Veterinary Medicine

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