RAPID AGGLUTINATION TEST (RAT) FOR THE DETECTION OF ANTI-Salmonella Typhimurium ANTIBODY IN SWINE

Authors

  • Rosecler Alves Pereira UFRGS
  • Marisa Macagnan UFRGS
  • Patricia Schwarz UFRGS
  • Claudio Wageck Canal UFRGS
  • Verônica Schmidt UFRGS

DOI:

https://doi.org/10.5216/cab.v11i3.6100

Keywords:

Animal Sanity

Abstract

Salmonella Typhimurium is an important agent isolated from cases of human food poisoning in Brazil. Animal-origin products are the main source of infection, and pork has been implicated in Salmonella transmission to humans. Programs to salmonella monitoring in swine have been carried out, using bacteriological and serological tests like ELISA. However, these tests are time-consuming and expensive. The rapid agglutination test (RAT) is cheaper, faster and easier. Aiming to standardize RAT to detect anti-salmonella antibodies in swine serum, 60 samples of swine serum were tested. They had previously displayed positive (30) or negative (30) results in the ELISA test. The results showed that RAT had sensitivity, specificity, predictive positive value and predictive negative value equal to 96.7% when non-diluted serum was tested. Thus, this test can be applied to detect antibodies against S. Typhimurium in swine serum.

KEYWORDS: Antibody, diagnosis, pig, rapid agglutination test, Salmonella Typhimurium.

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Author Biographies

Rosecler Alves Pereira, UFRGS

Médica Veterinária, Doutora em Ciências Veterinárias pelo Programa de Pós-Graduação em Ciências Veterinárias-UFRGS

Marisa Macagnan, UFRGS

Médica Veterinária, Doutora pelo Programa de Pós-Graduação em Ciências Veterinárias

Patricia Schwarz, UFRGS

Médica Veterinária, Doutora pelo Programa de Pós-Graduação em Ciências Veterinárias

Claudio Wageck Canal, UFRGS

Departamento de Patologia Clínica Veterinária. Áreas de atuação: virologia e sanidade animal

Verônica Schmidt, UFRGS

Departamento de Medicina Veterinária Preventiva. Áreas de atuação: produção e sanidade animal; saneamento aplicado.

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Published

2010-10-02

How to Cite

PEREIRA, R. A.; MACAGNAN, M.; SCHWARZ, P.; CANAL, C. W.; SCHMIDT, V. RAPID AGGLUTINATION TEST (RAT) FOR THE DETECTION OF ANTI-Salmonella Typhimurium ANTIBODY IN SWINE. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 3, p. 677–682, 2010. DOI: 10.5216/cab.v11i3.6100. Disponível em: https://revistas.ufg.br/vet/article/view/6100. Acesso em: 21 nov. 2024.

Issue

Vol. 11 No. 3 (2010)

Section

Veterinary Medicine

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