Occurrence of Escherichia coli in captive psittaciformes: antimicrobial susceptibility and virulence genes

Karine Louise Calaça; Renato Clini Cervi; Silvânia Andrade Reis; Iolanda Aparecida Nunes; Valéria de Sá Jayme; Maria Auxiliadora Andrade

doi:10.1590/1809-6891v21e-60433

Authors

Karine Louise Calaça Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0003-2923-3935
Renato Clini Cervi Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0003-2663-7221
Silvânia Andrade Reis Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0001-6563-976X
Iolanda Aparecida Nunes Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0001-8357-6969
Valéria de Sá Jayme Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0003-3755-9812
Maria Auxiliadora Andrade Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-8373-1671

DOI:

https://doi.org/10.1590/1809-6891v21e-60433

Abstract

Captive Psittaciformes may harbor Gram-negative bacteria in their digestive tract, mainly due to poor hygienic conditions and confinement. The present study was carried out with the objective of isolating and identifying Escherichia coli in samples collected from Psittaciformes cages in 50 commercial establishments in the metropolitan region of Goiania, with subsequent antimicrobial susceptibility testing and detection of virulence genes. A total of 141 samples of excreta and swab samples from feeders and water bowls were collected, totaling 423 samples. Escherichia coli was isolated from 9.7% (41/423) samples: 12% (17/141) in excreta, 8.5% (12/141) in feed, and 8.5% (12 /141) in waterers. To determine the susceptibility profile of E. coli isolates, resistance to ciprofloxacin 4.9% (2/41), gentamicin 17.0% (7/41), doxycycline 34.1% (14/41), florfenicol 34.1% (14/41), trimethoprim 39.0% (16/41), tetracycline 41.5% (17/41), enrofloxacin 43.9% (18/41), amoxicillin 48.8% (20/41), neomycin 61.0% (25/41), and sulfonamide 90.2% (37/41) was determined. In 20 isolates, resistance was determined at 4 or more antimicrobials, seven of excreta (7/17), five of feed (5/12), and eight of waterers (8/12). One of the isolates from the waterers showed resistance to all antimicrobials. The iss gene was detected in three isolates, the tsh gene in three, the papC gene in two, traT and eae genes were not detected. In this study, it can be concluded that Psittaciformes commercialized as pet are carry E. coli isolates resistant to most commonly used antimicrobials, mainly sulfonamides and neomycin, besides having virulence and serum resistance genes, which highlights the possibility of the to cause disease in humans.
Key words: APEC, multidrug resistance, virulence genes, wild birds, public health

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