Imunomarcação de metaloproteinases 9 (MMP-9) e 2 (MMP-2) e seus inibidores (TIMP-1) e (TIMP-2) no tecido mamário canino normal e neoplásico

Hugo Henrique Ferreira; Denise Caroline Toledo; Adriana Marques Faria; Diego Pereira Araújo; Andrigo Barboza di Nardi; Regiani Nascimento Gagno Porto; Veridiana Maria Brianezi Dignani de Moura

doi:10.1590/1809-6891v21e-58640

Authors

Hugo Henrique Ferreira Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-4431-9617
Denise Caroline Toledo Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0001-9807-8495
Adriana Marques Faria Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-8129-2476
Diego Pereira Araújo Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0003-0986-6890
Andrigo Barboza di Nardi Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0001-6463-2144
Regiani Nascimento Gagno Porto Universidade Federal de Goiás, Goiânia, GO, Brasil https://orcid.org/0000-0003-4640-3764
Veridiana Maria Brianezi Dignani de Moura Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil https://orcid.org/0000-0001-7128-7035

DOI:

https://doi.org/10.1590/1809-6891v21e-58640

Abstract

The aim of this study was to perform the immunostaining of MMP-9 and MMP-2 and its inhibitors, TIMP-1 and TIMP-2, on normal and neoplastic canine mammary tissue in order to evaluate the behavior of these proteins in extracellular matrix (ECM) remodeling in different neoplastic mammary types. Thus, 48 samples of canine mammary tissue were analyzed, 14 of which complex carcinomas, 13 tubulopapillary carcinomas, six single adenomas and 15 normal mammary tissue. There were differences in MMP-9, TIMP-1 and TIMP-2 according to mammary histomorphology, and MMP-9 presented increased immunoexpression in epithelial and stromal cells in tubulopapillary and complex carcinomas. TIMP-1 exhibited reduced immunostaining in the stromal cells of the complex carcinomas and TIMP-2 enhanced immunostaining in the epithelial cells of tubulopapillary carcinomas. There was a positive correlation between MMP-9 and TIMP-1 in epithelial and stromal cells regarding immunostaining intensity and number of labeled cells in the normal breast. There was a positive correlation between MMP-9 and TIMP-2 in the epithelial cells of tubulopapillary carcinomas. It is concluded that balanced activity between MMP-9, MMP-2, TIMP-1 and TIMP-2 maintains normal canine mammary tissue homeostasis while increased immunoexpression of MMP-9 and TIMP-2 and reduced TIMP- 1 in carcinomas suggest a favorable condition for tumor evolution.
Keywords: dog, mammary gland, neoplasm, matrix metalloproteinases.

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