INFLUENCE OF REFRIGERATION ON THE PRESERVATION OF THE TOTAL NUMBER OF HEMOCYTES OF SEA SHRIMPS Litopenaeus vannamei CULTIVATED USING SODIUM CITRATE

Authors

  • Andrea C. Gomes Barretto UFRPE
  • Lilian M.N. de Barros Góes
  • Dulcilene Lacerda do Nascimento
  • Paulo de Paula Mendes UFRPE
  • Eneida Wilcox Rêgo
  • Emiko Shinozaki Mendes

DOI:

https://doi.org/10.5216/cab.v13i4.5647

Keywords:

Aquaculture

Abstract

For carrying out blood cell count, the use anti-coagulants is necessary, and in the specific case of the hemolymph  in shrimp, there is still no standard solution to avoid its coagulation. Sodium citrate is frequently used as anti-coagulant, because it has been previously proven to be efficient in the preservation of the hemocytes of the sea shrimp Litopenaeus vannamei. In 140 samples of hemolymph with sodium citrate (10%) in the ratio of 1:1, collected at 2 stages of cultivation, in dry and wet season, were examined for HTC immediately after collection and then again after storage in the refrigerator. All data were analyzed using modeling mathematics formulas (P < 0.05). After storage in the refrigerator, the number of cells decreased considerably. Therefore, the anticoagulant sodium citrate only preserved the hemolymph of the shrimp
KEYWORDS: anticoagulant; haemolynph; shrimp.

Downloads

Download data is not yet available.

Author Biography

Andrea C. Gomes Barretto, UFRPE

Departamento de Medicina Veterinária

Laboratório de sanidade dos animais aquáticos

Downloads

Published

2012-12-14

How to Cite

BARRETTO, A. C. G.; GÓES, L. M. de B.; NASCIMENTO, D. L. do; MENDES, P. de P.; RÊGO, E. W.; MENDES, E. S. INFLUENCE OF REFRIGERATION ON THE PRESERVATION OF THE TOTAL NUMBER OF HEMOCYTES OF SEA SHRIMPS Litopenaeus vannamei CULTIVATED USING SODIUM CITRATE. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 13, n. 4, p. 520–524, 2012. DOI: 10.5216/cab.v13i4.5647. Disponível em: https://revistas.ufg.br/vet/article/view/5647. Acesso em: 22 nov. 2024.

Issue

Vol. 13 No. 4 (2012)

Section

Veterinary Medicine

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).