INFLUENCE OF HEPES ON IN VITRO MATURATION OF CANINE OOCYTES IN DIFFERENT STAGES OF THE ESTROUS CYCLE

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Abstract

Unlike other domestic animals, in vitro maturation (IVM) of canine oocytes still has limited success. The aim of this study was to evaluate the effect of HEPES on the in vitro maturation of dog oocytes in anestrus and diestrus. Ovaries were collected, aseptically isolated and transported refrigerated at a temperature of 4°C. Cumulus-oocyte complexe (COCs) from the two phases of estrous cycle were subjected to two treatments: medium TCM-199 with addition of 25 mM HEPES (TG) and medium without supplementation (CG). After 72 h of maturation, COCs were denuded, fixed and stained with Hoechst 33342 to assess nuclear maturation. The oocytes obtained from the anestrus and diestrus phases of the TG showed a higher frequency of oocytes in  metaphase II (M-II) (p <0.01) stage.Comparing the different reproductive status, it was observed that the oocytes obtained from the diestrus phase presented higher rates of GVBD (germinal vesicle breakdown) and M-II. Our results showed that HEPES preserves the viability and oocyte morphology essential for the acquisition of meiotic competence, increasing the M-II rates and that the oocytes obtained from the diestrus phase are better able to complete oocyte maturation.

Keywords: culture medium, biotechniques, estrous cycle, meiotic competence, bitches.

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Author Biographies

LEDA MARIA COSTA PEREIRA, Faculdade de Medicina Veterinária e Zootecnia- Universidade Estadual Julio de Mesquita Filho- UNESP, campus Botucatu

Departamento de Reprodução Animal e Radiologia Veterinária

Área Reprodução Animal 

Maria Denise Lopes, Faculdade de Medicina Veterinária e Zootecnia- Universidade Estadual Julio de Mesquita Filho- UNESP, campus Botucatu

Departamento de Reprodução Animal e Radiologia Veterinária

Área Reprodução Animal 

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Published

2019-10-24

How to Cite

PEREIRA, L. M. C.; BERSANO, P. R. O.; LOPES, M. D. INFLUENCE OF HEPES ON IN VITRO MATURATION OF CANINE OOCYTES IN DIFFERENT STAGES OF THE ESTROUS CYCLE. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 20, p. 1–9, 2019. Disponível em: https://revistas.ufg.br/vet/article/view/53732. Acesso em: 24 nov. 2024.

Issue

Vol. 20 (2019): Continuous publication

Section

MEDICINA VETERINÁRIA

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