MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE

Welington Erasmo de Lima e Silva; Evandro Santos Amanso; Rodolfo de Moraes Peixoto; João José de Simoni Gouveia; Gisele Veneroni Gouveia; Mateus Matiuzzi da Costa

doi:10.1590/1089-6891v20e-53193

MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE

Authors

Welington Erasmo de Lima e Silva Faculdade de Ciências Agrárias de Araripina (FACIAGRA), Araripina, Pernambuco, Brasil https://orcid.org/0000-0002-9159-3168
Evandro Santos Amanso Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, Brazil https://orcid.org/0000-0001-7665-8528
Rodolfo de Moraes Peixoto Instituto Federal do Sertão Pernambucano (IFSertãoPE), Petrolina, Pernambucano, Brasil https://orcid.org/0000-0002-5757-5935
João José de Simoni Gouveia Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, Brazil https://orcid.org/0000-0002-0438-094X
Gisele Veneroni Gouveia Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, Brazil https://orcid.org/0000-0003-1074-5711
Mateus Matiuzzi da Costa Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, Brazil https://orcid.org/0000-0002-9884-2112

DOI:

https://doi.org/10.1590/1089-6891v20e-53193

Abstract

This study aimed to evaluate the in vitro efficacy of commercial disinfectants on Staphylococcus spp., isolated from mastitis cases in goats, and to associate the observed resistance with the presence of the icaD gene. Broth microdilution was employed to evaluate the in vitro antimicrobial activity of the disinfectants, whereas the Congo Red technique was used for the evaluation of biofilm production and amplification of the icaD gene. All evaluated samples were sensitive to disinfectants, with the following ranges of activity: quaternary ammonium (0.13 – 21.33 µg/ml), chlorhexidine (4.00 – 313.00 µg/ml) and iodine (190.00 – 12500.00 µg/ml); however, the sodium hypochlorite-based disinfectant showed no bactericidal activity in the concentration range from 15.0 to 0.03 ?g/ml. The icaD gene presented a frequency of 14.7% in the isolate samples. Fisher's exact test showed a significant effect of the relation between the minimum bactericidal concentration value of the quaternary ammonium-based disinfectant and the presence/absence of the icaD gene (P <0.01). Commercial disinfectants with quaternary ammonium, chlorhexidine and iodine active ingredients presented in vitro activity even at concentrations lower than those recommended by the manufacturers. Therefore, the periodic evaluation of the sensitivity profile of the disinfectants must be performed.
Keywords: antimicrobial activity; biofilm; dairy goat; milking; chemical substances
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Published

2019-07-26

How to Cite

SILVA, W. E. de L. e; AMANSO, E. S.; PEIXOTO, R. de M.; GOUVEIA, J. J. de S.; GOUVEIA, G. V.; COSTA, M. M. da. MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 20, p. 1–8, 2019. DOI: 10.1590/1089-6891v20e-53193. Disponível em: https://revistas.ufg.br/vet/article/view/53193. Acesso em: 22 dec. 2024.

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Issue

Vol. 20 (2019): Continuous publication

Section

MEDICINA VETERINÁRIA

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