Suplementação de selênio na dieta e qualidade espermática do sêmen de touros brangus

Pedro Paulo Tsuneda; Bruno Hiroshi Tsuneda; Luciana Keiko Hatamoto Zervoudakis; Joanis Tilemahos Zervoudakis; Walter Augusto dos Santos Marinho; Moacir Ferreira Duarte Júnior; Eleonora Barbosa Araújo; Tathiana Ferguson Motheo; Luis Eduardo Senra e Silva

doi:10.1590/1089-6891v20e-48586

DIETARY SELENIUM SUPPLEMENTATION AND SPERM QUALITY IN BRANGUS BULLS SEMEN

Authors

Pedro Paulo Tsuneda Universidade Federal de Mato Grosso (UFMT), Cuiabá, Mato Grosso, Brasil https://orcid.org/0000-0001-8508-7931
Bruno Hiroshi Tsuneda Universidade Federal de Mato Grosso (UFMT), Cuiabá, Mato Grosso, Brasil https://orcid.org/0000-0002-7957-2452
Luciana Keiko Hatamoto Zervoudakis Universidade Federal de Mato Grosso (UFMT), Cuiabá, Mato Grosso, Brasil https://orcid.org/0000-0001-6030-096X
Joanis Tilemahos Zervoudakis Universidade Federal de Mato Grosso (UFMT), Cuiabá, Mato Grosso, Brasil https://orcid.org/0000-0002-1864-3237
Walter Augusto dos Santos Marinho Universidade Federal de Mato Grosso (UFMT), Cuiabá, Mato Grosso, Brasil https://orcid.org/0000-0003-1626-8629
Moacir Ferreira Duarte Júnior Universidade Federal de Mato Grosso (UFMT), Cuiabá, Mato Grosso, Brasil https://orcid.org/0000-0001-7383-1102
Eleonora Barbosa Araújo EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, Distrito Federal, Brasil https://orcid.org/0000-0002-3813-5272
Tathiana Ferguson Motheo Universidade Federal de Mato Grosso (UFMT), Cuiabá, Mato Grosso, Brasil https://orcid.org/0000-0002-2202-0331
Luis Eduardo Senra e Silva Universidade Federal de Mato Grosso (UFMT), Cuiabá, Mato Grosso, Brasil https://orcid.org/0000-0002-5703-0249

DOI:

https://doi.org/10.1590/1089-6891v20e-48586

Abstract

Abstract
The aim of the present study was to evaluate sperm quality of bulls supplemented with selenium (Se) in the diet. Sixteen Brangus bulls were randomly divided in two groups: control (GC) and Se (GSe - 0.1mg Se/kg dietary DM). The experiment lasted 75 days and the animals were supplemented for 60 days. Four semen collections (0, 30, 60 and 75 days) per animal, were performed during the experimental period. Sperm motility and vigor, plasma membrane integrity and functionality (hyposmotic swelling test - HOST) and sperm viability and acrosome reaction (triple staining -TRI) were assessed. After immediate analysis, samples were diluted in Tris-egg yolk extender with 5% glycerol, packed (40x106 spermatozoa / straw), chilled, frozen and stored in liquid nitrogen until analysis. After thawing, sperm motility and vigor, HOST and TRI were performed. No significance was noticed on sperm vigor, HOST and TRI of fresh and post-thawed semen after dietary selenium supplementation. However, sperm motility decreased in GSe compared to GC in fresh (P = 0.0035) and post- thawed (P = 0.0067) semen samples after 60 days of supplementation. Therefore, dietary selenium supplementation was ineffective to improve semen parameters of fresh and post-thawed semen of Brangus bulls.
Keywords: antioxidant, cryopreservation, spermatozoa.

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Author Biography

Eleonora Barbosa Araújo, EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, Distrito Federal, Brasil

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Published

2019-05-24

How to Cite

TSUNEDA, Pedro Paulo; TSUNEDA, Bruno Hiroshi; ZERVOUDAKIS, Luciana Keiko Hatamoto; ZERVOUDAKIS, Joanis Tilemahos; MARINHO, Walter Augusto dos Santos; JÚNIOR, Moacir Ferreira Duarte; ARAÚJO, Eleonora Barbosa; MOTHEO, Tathiana Ferguson; SILVA, Luis Eduardo Senra e. DIETARY SELENIUM SUPPLEMENTATION AND SPERM QUALITY IN BRANGUS BULLS SEMEN. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 20, p. 1–9, 2019. DOI: 10.1590/1089-6891v20e-48586. Disponível em: https://revistas.ufg.br/vet/article/view/48586. Acesso em: 5 dec. 2025.

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Issue

Vol. 20 (2019): Continuous publication

Section

ANIMAL SCIENCE

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