Ação dos crioprotetores glicose, trealose e quitosana na manutenção da viabilidade de células de Escherichia coli, Saccharomyces cerevisiae após liofilização

Andressa Barella de Freitas; Creciana Maria Endres; Diane Martini; Andréia Paula Dal Castel

doi:10.1590/1809-6891v21e-47464

Authors

Andressa Barella de Freitas Senai https://orcid.org/0000-0003-4713-974X
Creciana Maria Endres SENAI https://orcid.org/0000-0002-5662-2197
Diane Martini SENAI https://orcid.org/0000-0002-2564-6428
Andréia Paula Dal Castel SENAI https://orcid.org/0000-0003-1221-8843

DOI:

https://doi.org/10.1590/1809-6891v21e-47464

Abstract

Abstract
Lyophilization is considered one of the safest techniques for acquiring stability of culture strains. For this process, cryoprotectants, which are substances that protect cell structures during the period of freezing, thawing and dehydration, may be used. The objective of the present work was to evaluate the performance of glucose, trehalose, and chitosan as cryoprotectants for Escherichia coli e Saccharomyces cerevisiae. For statistical treatment, ANOVA and Tukey's test were used with the software Bioestat, version 5.3. For Escherichia coli Trealose presented better performance after lyophilization, but none of the treatments proved to be effective in prolonging viability up to 60 days of storage. For Saccharomyces cerevisiae all treatments were satisfactory throughout the 60 days evaluated.
Key words: Conservation. Stability of strains. Lyophilization. Microorganisms.

Downloads

Download data is not yet available.

References

Morais JAS, Queiroz MFS, Keli A, Vega A, Fiorentini G, Canesin RC, Reis RA, Berchielli TT. Effect of supplementation frequency on intake, behavior and performance in beef steers grazing Marandu grass. Anim Feed Sci Technol 2014; 189: 63-71.

Bonadimann R, Moraes EHBK, Moraes KAK, Pina DS, Oliveira AS, Meneses DM, Stinguel H, Almeida CM. Frequency of concentrate supplementation for grazing beef cattle. Semina: Ciênc Agrár 2017 ; 38: 21412150.

Silva-Marques RP, Zervoudakis JT, De Paula NF, Hatamoto-Zervoudakis LK, Silva PIJLR, Matos NBN. Effects of protein-energetic supplementation frequency on growth performance and nutritional characteristics of grazing beef cattle. Trop Anim Health Prod 2018; 50: p 495-501.

Atkinson RL, Toone CD, Robinson TJ, Harmon DL, Ludden PA. Effects of ruminal protein degradability and frequency of supplementation on nitrogen retention, apparent digestibility, and nutrient flux across visceral tissues in lambs fed low-quality forage. J Anim Sci 2010; 88: 727-736.

Farmer CG, Cochran RC, Nagaraja TG, Titgemeyer EC, Johnson DE, Wickersham TA. Ruminal and host adaptations to changes in frequency of protein supplementation. J Anim Sci 2004; 82 : 895-903.

NRC- National Research Council. Nutrient requirements of beef cattle. 7a Ed. Washington DC: National Academic Press ; 1996. 242p. English.

Cappelle ER, Valadares Filho SC, Coelho Da Silva JF, Cecon PR. Estimativas do valor energético a partir de características químicas e bromatológicas dos alimentos. Rev Bras Zootec 2001; 30: 1837-1856.

Valadares Filho SC, Magalhães KA, Rocha Junior VR, et al. Tabelas brasileiras de composição de alimentos para bovinos. 2.ed. Viçosa, MG: UFV; 2006. 329p.

Sniffen CJ, O'connor JD, Van Soest PJ, Fox DG, Russell JB. A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. J Anim Sci 1992; 70: 3562-3577.

Ribeiro SS, Vasconcelos JT, Morais MG, Ítavo CBCF, Franco GL. Effects of ruminal infusion of slow-release polymer-coated urea or conventional urea on apparent nutrient digestibility, in situ degradability, and rumen parameters in cattle fed low-quality hay. Anim Feed Sci Technol 2011; 164: 53-61.

Huntington JA, Givens DI. The in situ technique for studying the rumen degradation of feeds: a review of the procedure. Nutr Res Rev Series B 1995; 65: 63-93.

AOAC. Official methods of analysis, 17th ed. Gaithersburg, MD : Association of Official Analytical Chemists; 2000. English.

Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polyssacarides in relation to animal nutrition. J Anim Sci 1991; 74: 3583-3597.

NRC- National Research Council. Nutrient requirements of dairy cattle. 7a Ed. Washington DC: National Academy Press; 2001. 381p. English.

Hall MB. Calculation of non-structural carbohydrate content of feeds that contain non-protein nitrogen. Gainesville: University of Florida ; 2000. p.A-25 (Bulletin, 339). English.

Fenner H. Method for determining total volatile bases in rumen fluid by steam distillation, J Dairy Sci 1965; 48: 249-251.

Mcguire DL, Bohnert DW, Schauer CS, Falck SJ, Cooke RF. Daily and alternate day supplementation of urea or soybean meal to ruminants consuming low-quality cool-season forage: I- Effects on efficiency of nitrogen use and nutrient digestion. Livest Sci 2013; 155: 205-213.

Drewnoski ME, Poore MH, Benson GA.Effect of frequency of supplementation of a soyhulls and corn gluten feed blend on hay intake and performance of growing steers. Anim Feed Sci Technol 2011; 164: 38-44.

Moore JA, Brant MH, Kunkle WE, Hopkins DI. Effects of supplementation on voluntary forage intake, diet digestibility and animal performance. J Anim Sci 1999; 77: 122-135.

Dixon RM, Stockdale CR. Associative effects between forages and grains: consequences for feed utilization. Aust J Agric Res 1999; 50: 757- 774.

Bohnert DW, Schauer RC, Bauer ML, Delcurto T. Influence of rumen protein degradability and supplementation frequency on steers consuming low quality forage: site of digestion and microbial efficiency. J Anim Sci 2002; 80:2967-2977.

Schauer CS, Bohnert DW, Ganskopp DC, Richards CJ, Falck SJ. Influence of protein supplementation frequency on cows consuming low-quality forage: Performance, grazing behavior and variation in supplement intake. J Anim Sci 2005; 83: 1715-1725.

Franco GL, Andrade PA, Bruno Filho JR, Diogo JMS. Parâmetros ruminais e desaparecimento da FDN da forragem em bovinos suplementados em pastagem na estação das águas. Rev Bras Zootec 2002; 31: 2340-2349.

Ortignes I, Fontenot JP, Ferry JG. Digesta flows in sheep fed poor-quality hay supplemented with urea and carbohydrates. J Anim Sci 1988; 66: 975-985.

Galyean ML, Owens FN. Effects of diet composition and level of feed intake on site and extent of digestion in ruminants. In: Tsuda T, Sasaki Y, Kawashima R, editors. Physiological aspects of digestion and metabolism in ruminants. New York : Academic Press ; 1991. p.483-514.

Hess BW, Park KK, Krysl MB, Judkins MB, Mccracken BA, Hanks DR. Supplemental protein for beef cattle grazing dormant intermediate wheatgrass pasture: effects on nutrient quality, forage intake, digesta kinetics, grazing behavior, ruminal fermentation, and digestion. J Anim Sci 1994; 72:2213-2223.

Hiltner P, Dehority BA. Effects of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria. Appl Environ Microbiol 1983; 46: 642-648.

Bohnert DW, Delcurto T, Clark AA, Merrill ML, Falck SJ, Harmon DL. Protein supplementation of ruminants consuming low-quality cool- or warm-season forage: differences in intake and digestibility. J Anim Sci 2011; 89: 3707-3717.

Cappellozza BI, Bohnert DW, Schauer CS, Falck SJ, Vanzant ES, Harmon DL, Cooke RF. Daily and alternate day supplementation of urea or soybean meal to ruminants consuming low-quality cool-season forage: II. Effects on ruminal fermentation. Livest Sci 2013; 155: 214-222.

Satter MK, Slyter JJ. Ruminal fermentation pattern bacterial population and ruminal degradation of feed ingredients as influenced by ruminal ammonia concentration. J Anim Sci 1974; 38: 310-320.

Reynolds CK, Kristensen NB. Nitrogen recycling through the gut and the nitrogen economy of ruminants: An asynchronous symbiosis. J Anim Sci 2008; 86: E293-E305.

Wickersham TA, Titgemeyer EC, Cochran RC, Wickersham EE, Moore ES. Effect of frequency and amount of rumen-degradable intake protein supplementation on urea kinetics and microbial use of recycled urea in steers consuming low-quality forage. J Anim Sci 2008; 86: 3089-3099.