Action of cryoprotectors glucose, trealose and quitosan in maintaining viability of Escherichia coli, Saccharomyces cerevisiae cells after lyophilization

Authors

Abstract

Abstract
Lyophilization is considered one of the safest techniques for acquiring stability of culture strains. For this process, cryoprotectants, which are substances that protect cell structures during the period of freezing, thawing and dehydration, may be used. The objective of the present work was to evaluate the performance of glucose, trehalose, and chitosan as cryoprotectants for Escherichia coli e Saccharomyces cerevisiae. For statistical treatment, ANOVA and Tukey's test were used with the software Bioestat, version 5.3. For Escherichia coli Trealose presented better performance after lyophilization, but none of the treatments proved to be effective in prolonging viability up to 60 days of storage. For Saccharomyces cerevisiae all treatments were satisfactory throughout the 60 days evaluated.
Key words: Conservation. Stability of strains. Lyophilization. Microorganisms.

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Author Biographies

Andressa Barella de Freitas, Senai

Tecnóloga em alimentos, especialista em inovação e tecnologia, departamento de Microbiologia de alimentos.

Creciana Maria Endres, SENAI

Mestre em ciência e tecnologia de alimentos, docente da faculdade SENAI.

Diane Martini, SENAI

Orientadora. Departamento de microbiologia.

Andréia Paula Dal Castel, SENAI

Laboratório de Microbiologia.

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Published

2020-02-05

How to Cite

FREITAS, A. B. de; ENDRES, C. M.; MARTINI, D.; DAL CASTEL, A. P. Action of cryoprotectors glucose, trealose and quitosan in maintaining viability of Escherichia coli, Saccharomyces cerevisiae cells after lyophilization. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 21, 2020. Disponível em: https://revistas.ufg.br/vet/article/view/47464. Acesso em: 21 nov. 2024.

Issue

Vol. 21 (2020): Continuous publication

Section

CIÊNCIAS BIOLÓGICAS

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