DIARRHEAGENIC Escherichia coli IN RAW MILK, WATER, AND CATTLE FECES IN NON-TECHNIFIED DAIRY FARMS

Authors

  • Laryssa Freitas Ribeiro UNESP – Univ. Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Via de acesso Paulo Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil. http://orcid.org/0000-0003-1943-7897
  • Mayhara Martins Cordeiro Barbosa Instituto Federal de Educação, Ciência e Tecnologia do Ceará (IFCE) – Avenida José de Freitas Queiroz, nº 5000, Bairro Cedro, 63902-580 Quixadá, CE, Brazil http://orcid.org/0000-0002-1970-8266
  • Fernanda de Rezende Pinto Faculdade de Veterinária; Universidade Federal de Pelotas (UFPEL) – Campus Universitário Capão do Leão, s/n, Prédio nº 01, 96160-000 Pelotas, RS, Brazil http://orcid.org/0000-0002-0794-1984
  • Leticia Fernanda Lavezzo UNESP – Univ. Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Via de acesso Paulo Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil. http://orcid.org/0000-0002-1461-6362
  • Gabriel Augusto Marques Rossi UNESP – Univ. Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Via de acesso Paulo Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil. http://orcid.org/0000-0001-7967-7628
  • Henrique Meiroz de Souza Almeida UNESP – Univ. Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Via de acesso Paulo Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil. http://orcid.org/0000-0002-7631-4271
  • Luiz Augusto do Amaral UNESP – Univ. Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Via de acesso Paulo Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil. http://orcid.org/0000-0002-5297-4961

Abstract

This study focused on detecting diarrheagenic Escherichia coli, enteropathogenic E. coli (EPEC), Shiga-toxin-producing E. coli (STEC), enterohemorrhagic E. coli (EHEC or STEC:EPEC), enterotoxigenic E. coli (ETEC), and enteroaggregative E. coli (EAEC) in raw milk, water, and cattle feces sampled from non-technified dairy farms located in the northeastern São Paulo State, Brazil. Thirty-six water samples were collected at different points, namely, water wells (8 samples), water intended for human consumption (8 samples), water from milking parlor (8 samples), and water intended for animal consumption (7 samples), headwaters (1 sample), rivers (3 samples), and reservoirs (1 sample). Three raw milk samples were taken directly from bulk tanks in each farm, totalizing 24 samples. Feces samples were collected using rectal swabs from 160 bovines (20 animals per farm). E. coli was detected in 128 feces samples (80%), 16 raw milk samples (66.67%), and 20 water samples (55.56%). STEC (26 samples, 16.25%), EPEC (10 samples, 6.25%), STEC: EPEC (5 samples, 3.13%), and STEC: ETEC (1 sample, 0.63%) were the most prevalent strains detected in samples from cattle feces. EPEC, STEC, and STEC: EPEC strains were detected in 4.17% (1 sample), 16.67% (4 samples), and 4.17% (1 sample) of raw milk samples, respectively. STEC strains were detected in water used in the milking parlor, while no EAEC strain was detected. As a conclusion, cattle feces are important contamination sources of pathogenic E. coli in non-technified dairy farms and, consequently, cross-contamination among feces, water, and/or raw milk can occur. The use of quality water and hygienic practices during milking are recommended to avoid contamination since pathogens can be transmitted to humans via raw milk or raw milk cheese ingestion.
Keywords: EAEC, ETEC, EPEC, STEC, public health

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Author Biography

Laryssa Freitas Ribeiro, UNESP – Univ. Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Via de acesso Paulo Castellane, s/n, 14884-900 Jaboticabal, SP, Brazil.

Graduado em Medicina Veterinária pela Faculdade de Ciência Agrárias e Veterinárias de Jaboticabal (UNESP/Jaboticabal) em 2010, sendo bolsista de Iniciação Científica pela FAPESP. Mestre em Medicina Veterinária (área Medicina Veterinária Preventiva; Higiene e Inspeção Sanitária de Alimentos) com título obtido em 2014 sendo bolsista CAPES. Doutorado em andamento na mesma área e mesma instituição, com bolsa de doutorado financiada pela FAPESP. Experiência na área de: Medicina Veterinária Preventiva; Tecnologia, Microbiologia e Inspeção Sanitária de Produtos de Origem Animal; Cisticercose. Até o momento possui 19 artigos completos publicados ou aceitos para publicação em períódicos e ais de 50 resumos apresentados em congressos e simpósios.

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Published

2019-09-23

How to Cite

RIBEIRO, L. F.; BARBOSA, M. M. C.; PINTO, F. de R.; LAVEZZO, L. F.; ROSSI, G. A. M.; ALMEIDA, H. M. de S.; DO AMARAL, L. A. DIARRHEAGENIC Escherichia coli IN RAW MILK, WATER, AND CATTLE FECES IN NON-TECHNIFIED DAIRY FARMS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 20, p. 1–9, 2019. Disponível em: https://revistas.ufg.br/vet/article/view/47449. Acesso em: 27 jul. 2024.

Issue

Vol. 20 (2019): Continuous publication

Section

MEDICINA VETERINÁRIA

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