EFECT OF MINIMUM CONTAMINATION MEDIUM (MMC) AND MODIFIED EGG YOLK LACTOSE (LGM) EXTENDERS ON CANINE SEMEN VIABILITY AFTER COOLING AT 4 ºC FOR 24 HOURS IN EQUITAINER®
DOI:
https://doi.org/10.5216/cab.v12i1.4692Keywords:
Animal reproductionAbstract
The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC. No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics.KEYWORDS: Dogs, sperm, cooling.
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Published
2011-03-31
How to Cite
BARCELLOS, B. P.; ROCHA, M. A.; JAMAS, L.; BRANDÃO, F. Z.; NOGUEIRA, L. G.; PINHO, T. G. de. EFECT OF MINIMUM CONTAMINATION MEDIUM (MMC) AND MODIFIED EGG YOLK LACTOSE (LGM) EXTENDERS ON CANINE SEMEN VIABILITY AFTER COOLING AT 4 ºC FOR 24 HOURS IN EQUITAINER®. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 12, n. 1, p. 126–133, 2011. DOI: 10.5216/cab.v12i1.4692. Disponível em: https://revistas.ufg.br/vet/article/view/4692. Acesso em: 22 nov. 2024.
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Veterinary Medicine
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