POLYCLONAL ANTIBODIES FOR THE IMMUNODIAGNOSTIC OF BOVINE VIRUSES

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Abstract

The objective of the study was to produce polyclonal antibodies to bovine viruses and to test the reactivity in immunoassays such as immunofluorescence, immunoperoxidase and slot blot. Bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine herpesvirus type 1, 2 and 5 (BoHV-1, BoHV-2 and BoHV-5) blue tongue virus (BTV) and vaccinia virus (VACV) were amplified in cell culture and used to immunize rabbits. Animals were immunized at regular intervals and the hyper immune serum produced was used as primary antibody in the immunoassays. The working dilution for each antiserum was determined by limiting serial dilution and varied from 1: 800 to 1: 51,200. The highest dilutions were observed for immunoperoxidase, when compared with immunofluorescence and slot blot. Dilutions lower than 1:800 were associated with the presence of nonspecific reactions. Anti-BoHV-1, -BoHV-5, -BVDV and -BRSV antisera reacted against heterologous isolates in immunofluorescence and immunoperoxidase assays. It is concluded that the produced polyclonal antibodies have high concentrations of virus-specific antibodies and are an alternative for use in immunoassays.
Keywords: antiserum, viral detection, immunoassay, diagnostic.

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Author Biographies

Tatiane Goulart de Lima, Programa de Pós-Graduação em Ciência Animal, Universidade Federal do Pampa

Acadêmica do PPGCA, Campus Uruguaiana, Universidade Federal do Pampa, Uruguaiana, RS.

Luana Marchi Quadros, Programa de Pós-Graduação em Ciência Animal, Universidade Federal do Pampa

Acadêmica do PPGCA, Campus Uruguaiana, Universidade Federal do Pampa, Uruguaiana, RS.

Mário Celso Sperotto Brum, Laboratório de VirologiaCurso de Medicina VeterináriaUniversidade Federal do PampaCampus Uruguaiana - UNIPAMPAUruguaiana, RS - CEP 97.508.000mariobrum@unipampa.edu.br

Docente Imunologia Veterinária e Doenças Víricas

Mestre em Virologia Animal - UFSM

Doutor em Virologia Animal - UFSM

 

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Published

2019-09-23

How to Cite

DE LIMA, T. G.; QUADROS, L. M.; MALLMANN, L. A.; BRUM, M. C. S. POLYCLONAL ANTIBODIES FOR THE IMMUNODIAGNOSTIC OF BOVINE VIRUSES. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 20, p. 1–9, 2019. Disponível em: https://revistas.ufg.br/vet/article/view/46625. Acesso em: 22 nov. 2024.

Issue

Vol. 20 (2019): Continuous publication

Section

MEDICINA VETERINÁRIA

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