ANTIMICROBIAL ACTIVITY OF THE HONEY OF JATAÍ BEES (Tetragonisca angustula) IN CULTIVATION OF MICROORGANISMS OF THE HEARING CONDUIT OF DOMESTIC CANINES (Canis familiaris)

Abstract

The present work aimed to investigate, in vitro, the efficacy of the honey of Tetragonisca angustula, against the microorganisms found in the auditory canal inflammations of dogs. The  samples were collected through a sterile swab of secretion of the auditory canal of dogs with clinical otitis. The material was fixed in glass slide by performing the smear, Gram stained, and submitted to microscopic identification. The samples were inoculated in Tryptone Soya Broth culture media and incubated at 37°C for 24 hours. After verifying that there was growth, the samples were cultured in Petri’s plates containing Chapman, Levine and Mueller-Hinton, and incubated at 37°C for 24 hours. Filter paper discs were adapted, sterilized in autoclave and embedded in honey for the antibiogram. The disks impregnated with honey were placed in Petri’s plates containing the contamined material and again incubated at 37°C for 24 hours. This experiment verified the formation of inhibition halos in the antibiograma with the honey of the Tetragonisca angustula, showing to be effective in the control of Staphylococcus sp, Bacillus sp and yeasts.

KEYWORDS: Honey, otitis, treatment, Tetragonisca angustula.