OMPARISON BETWEEN TWO MEDIUM FOR EMBRYO TRANSFER IN MARES FROM MANGALARGA MARCHADOR BREED

Authors

  • José Renato Costa Caiado UENF
  • Mauricio Fraga van Tilburg UENF
  • Francisco Aloísio Fonseca UENF
  • José Frederico Straggiotti Silva UENF
  • Bruno Fagundes UNIG
  • Marcus Antonio Pessanha Barreto UENF

DOI:

https://doi.org/10.5216/cab.v10i3.4514

Keywords:

Animal reproduction

Abstract

Different buffers are used in the solution for washing and embryo maintenance during the procedures of equine embryo transfer (EET). The buffers carbonate, phosphate, and zwitterionic are the most used. During the procedures of transference, it is common to keep the embryos into the maintenance solution during a time longer than previewed. This experiment was realized with the objective of comparing the costs and benefits of using two solutions with different buffers for embryo transfer in mares of the breeding Mangalarga Marchador. The solutions are DPBS (phosphate) and Embriocare® (zwitterionic). The embryos were divided randomly in two treatment , zwitterionic and phosphate, and kept in the solution for 0, 30, 60, and 120 minutes. The results indicate that both solutions are efficient (P > 0.05) in the processes of scanning, washing, maintenance at ambient temperature for different time periods (0 to 120 minutes), and inovulation of equine embryos under tropical conditions. The pregnancy rates using the solution were 65% and 69.7%. The greatest pregnancy rate using DPBS was obtained with embryos kept in the solution for 60 minutes (P < 0.05). Using zwitterionic buffer, the greatest rate of pregnancy was obtained with embryos kept during 30 minutes in the solution (P < 0.05). Since Embriocare® is more expensive, DPBS presented the best cost-benefit ratio.

KEY WORDS: Embryo transfer, equine, Mangalarga Marchador, phosphate and zwitterionic buffer.

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Author Biographies

José Renato Costa Caiado, UENF

Doutor em Produção Animal, LSA-CCTA-UENF

Mauricio Fraga van Tilburg, UENF

Doutor em Produção Aminal na área de Biotecnologia da Reprodução pela Universidade Estadual do Norte Fluminense Darcy Ribeiro. LRMGA-CCTA-UENF

Francisco Aloísio Fonseca, UENF

PHD em Animal Science pela North Carolina State University. Professor associado LZNA-CCTA -UENF

José Frederico Straggiotti Silva, UENF

Doutorado em  Medicina Veterinária em Tierärztlichen Hochschule Hannover. Professor associado LRMGA-CCTA-UENF.

Bruno Fagundes, UNIG

Doutor em Produção Aminal na área de Biotecnologia da Reprodução pela Universidade Estadual do Norte Fluminense Darcy Ribeiro. Professor da UNIG.

Marcus Antonio Pessanha Barreto, UENF

Doutor em Produção Aminal pela Universidade Estadual do Norte Fluminense Darcy Ribeiro. LRMGA-CCTA_UENF

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Published

2009-09-30

How to Cite

CAIADO, J. R. C.; VAN TILBURG, M. F.; FONSECA, F. A.; SILVA, J. F. S.; FAGUNDES, B.; BARRETO, M. A. P. OMPARISON BETWEEN TWO MEDIUM FOR EMBRYO TRANSFER IN MARES FROM MANGALARGA MARCHADOR BREED. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 3, p. 938–946, 2009. DOI: 10.5216/cab.v10i3.4514. Disponível em: https://revistas.ufg.br/vet/article/view/4514. Acesso em: 8 jul. 2024.

Issue

Vol. 10 No. 3 (2009)

Section

Veterinary Medicine

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