OMPARISON BETWEEN TWO MEDIUM FOR EMBRYO TRANSFER IN MARES FROM MANGALARGA MARCHADOR BREED

Authors

  • José Renato Costa Caiado Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • Mauricio Fraga Van Tilburg Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • Francisco Aloísio Fonseca Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • José Frederico Straggiotti Silva Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • Bruno Fagundes Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • Marcus Antonio Pessanha Barreto Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil

Keywords:

Animal reproduction

Abstract

Different buffers are used in the solution for washing and embryo maintenance during the procedures of equine embryo transfer (EET). The buffers carbonate, phosphate, and zwitterionic are the most used. During the procedures of transference, it is common to keep the embryos into the maintenance solution during a time longer than previewed. This experiment was realized with the objective of comparing the costs and benefits of using two solutions with different buffers for embryo transfer in mares of the breeding Mangalarga Marchador. The solutions are DPBS (phosphate) and Embriocare® (zwitterionic). The embryos were divided randomly in two treatment , zwitterionic and phosphate, and kept in the solution for 0, 30, 60, and 120 minutes. The results indicate that both solutions are efficient (P > 0.05) in the processes of scanning, washing, maintenance at ambient temperature for different time periods (0 to 120 minutes), and inovulation of equine embryos under tropical conditions. The pregnancy rates using the solution were 65% and 69.7%. The greatest pregnancy rate using DPBS was obtained with embryos kept in the solution for 60 minutes (P < 0.05). Using zwitterionic buffer, the greatest rate of pregnancy was obtained with embryos kept during 30 minutes in the solution (P < 0.05). Since Embriocare® is more expensive, DPBS presented the best cost-benefit ratio.

KEY WORDS: Embryo transfer, equine, Mangalarga Marchador, phosphate and zwitterionic buffer.

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Published

2009-09-30

How to Cite

CAIADO, José Renato Costa; TILBURG, Mauricio Fraga Van; FONSECA, Francisco Aloísio; SILVA, José Frederico Straggiotti; FAGUNDES, Bruno; BARRETO, Marcus Antonio Pessanha. OMPARISON BETWEEN TWO MEDIUM FOR EMBRYO TRANSFER IN MARES FROM MANGALARGA MARCHADOR BREED. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 3, p. 938–946, 2009. Disponível em: https://revistas.ufg.br/vet/article/view/4514. Acesso em: 6 dec. 2025.

Issue

Vol. 10 No. 3 (2009)

Section

Veterinary Medicine

License

Copyright (c) 2009 Brazilian Animal Science/ Ciência Animal Brasileira

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