PHYSICAL, BIOCHEMICAL AND CYTOLOGICAL CHARACTERISTICS OF SYNOVIAL FLUID WITH INDUCED SYNOVITIS MODEL IN PONIES

Authors

  • Ricardo Pozzobon Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brasil
  • Karin Erica Brass Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brasil
  • Rafael Almeida Fighera Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brasil
  • Flávio Dessessards De La Corte Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brasil

Keywords:

Animal Sanity

Abstract

The aim of this study was to describe the changes of the synovial fluid caused by experimental synovitis induced by an intra-articular injection of 0.5 mL of Freund’s complete adjuvant in six adult ponies. The physical-chemical and cytological parameters were evaluated before the synovitis induction (T0), 5 days after induction (T1) and, after that, at 12 (T2), 24 (T3), 48 (T4), 72 (T5), 96 (T6) and 120 hours (T7). The contra lateral joint served as healthy control. The protein, viscosity and mucin precipitation quality did not differ (P>0.05) among the joints in T0. At T1, the synovial fluid of the joints with synovitis showed an increased protein concentration (6.5 g/dl), low viscosity and low mucin precipitate quality, which remained stable until T7. Viscosity and mucin precipitate quality did not change in the synovial fluid of healthy joints until the end of the experiment. Due to repeated arthrocentesis, protein increased (p <0.05) at T6 and T7. Joints with synovitis had an increased total nucleated cell count (>700 cells/mm3, p <0.05) at T1, that decreased at T3, remaining on an inflammatory level during the observation period. The differential cell count showed neutrophil predominance and presence of reactive mesothelial cells. The intra-articular injection of 0.5 mL of Freund’s complete adjuvant produced a neutrophilic synovitis of moderate to severe intensity, similar to the natural synovitis, reinforcing the effectiveness of this model in the study of the pathophysiology and treatment of joint diseases.
KEY WORDS: Equine orthopedics, synovitis, synovial fluid.

Downloads

Download data is not yet available.

Downloads

Published

2009-12-20

How to Cite

POZZOBON, Ricardo; BRASS, Karin Erica; FIGHERA, Rafael Almeida; CORTE, Flávio Dessessards De La. PHYSICAL, BIOCHEMICAL AND CYTOLOGICAL CHARACTERISTICS OF SYNOVIAL FLUID WITH INDUCED SYNOVITIS MODEL IN PONIES. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 4, p. 1303–1309, 2009. Disponível em: https://revistas.ufg.br/vet/article/view/4491. Acesso em: 11 feb. 2026.

Issue

Vol. 10 No. 4 (2009)

Section

Veterinary Medicine

License

Copyright (c) 2009 Brazilian Animal Science/ Ciência Animal Brasileira

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).